Abstract
Moisture stress during crop growth has been identified as a major impediment to long-term agricultural productivity. Crop losses vary depending on the severity and duration of the drought, as well as the stage of growth. The objective of the present investigation was to identify superior genotypes of horse gram with moisture stress tolerance having high yield and quality, especially suitable for the south-central laterite region of Kerala (India) as well as determine the nature and extent of diversity amongst the genotypes under investigation. In this study, we investigated the response of thirty genotypes of horse gram to their moisture stress tolerance at the seedling stage using PEG 6000 osmoticum in the laboratory and by withholding irrigation at the reproductive stage in the field. The genotypes were evaluated for six different morpho-physiological traits as well as various biometrical, physiological, biochemical and seed quality characters. Results of the analysis of variance revealed that the genotypes varied significantly for all traits. To know the magnitude of genetic diversity existing in genotypes, the Mahanalobis D2 statistic was also performed. The results from both experiments indicated that the genotypes IC22785 and IC139464 have the potential to be used in breeding programs for drought tolerance, as these genotypes recorded superior performance under high moisture stress conditions. The promising genotypes belonged to cluster III in the divergence analysis. Superior segregants may be obtained by crossing clusters I and III, as the highest inter-cluster distance was between them.
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All authors contributed to the study’s conception and design. Material preparation, data collection and analysis were performed by Mr. Visakh Ravindran Lalithambika and Dr. Mangalath Radhamony Bindu, the investigation was supervised and validated by Dr. Mangalath Radhamony Bindu, Dr. Roy Stephen and Dr. Poornima Yadav. The first draft of the manuscript was written by Mr. Visakh Ravindran Lalithambika and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Lalithambika, V.R., Bindu, M.R., Stephen, R. et al. Evaluation of horse gram (Macrotyloma uniflorum) for moisture stress tolerance at seedling and reproductive stage. Biologia 78, 707–725 (2023). https://doi.org/10.1007/s11756-022-01258-6
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DOI: https://doi.org/10.1007/s11756-022-01258-6