Abstract
Rotylenchus brevicaudatus was identified morphologically from soil samples collected around the rhizosphere of wild grass in the North West Province of Botswana. This species is characterized by a rounded lip region bearing four incisures, with slight depression. The basal annuli are divided into six to seven blocks in the ventral view. Tail is rounded with 8–14 μm length, stylet is 21–23 μm in length, spicules 25–26 μm, and gubernaculum 9–11 μm long. The nblast analysis based on the D2-D3 segment of 28S rDNA revealed 96% identity of the Botswanan populations of R. brevicaudatus to the Australian R. brevicaudatus (JX015419, JX015420). The phylogenetic analysis put the Botswanan and Australian population of R. brevicaudatus with a 1.00 posterior probability support. This is the first record of R. brevicaudatus in Botswana. In addition, SEM photographs of R. brevicaudatus are provided for the first time.
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Acknowledgements
The authors thank the research support plans “PAIUJA 2019/2020: EI_RNM02_2019” and “POAIUJA 2021/2022: EI_RNM02_2021” of the University of Jaén, Spain. The SEM pictures were obtained with the assistance of technical staff (Amparo Martínez-Morales) and equipment of “Centro de Instrumentación Científico-Técnica (CICT)” from the University of Jaén. In addition, the authors acknowledge Aquaculture Research Unit for the microscopical facilities.
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This study was financially supported by the University of Jaén (Spain) for the SEM photographs. The rest of the project was supported by ES.
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ES conducted the study, identified the species and analysed the data, wrote and revised the manuscript. JA revised the manuscript and took the LM and SEM of the species. ES and JA contributed to the final draft of the manuscript.
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Shokoohi, E., Abolafia, J. Observation on the Rotylenchus brevicaudatus Colbran, 1962 from Botswana, with the first SEM of the species. Biologia 77, 3501–3509 (2022). https://doi.org/10.1007/s11756-022-01240-2
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DOI: https://doi.org/10.1007/s11756-022-01240-2