Abstract
Synthetic polymers, such as polyethylene (PE), are produced daily in large quantities and, due to their unique properties, like durability and low cost, consumed in various capacities worldwide, subsequently accumulating in terrestrial and marine environments. In recent years, several studies have focused on the toxicological effects of these plastic particles, and bioremediation approaches for the removal of these wastes. Easy access to nano and micro-sized PE particles in these approaches is crucial and urgent. In this research, we report a simple, fast, and cost-effective method for producing nano polyethylene particles (NPE) that are stable and capable of dispersion in water without chemical modifications. PE particles were characterized by dynamic light scattering (DLS), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier Transform Infrared (FTIR) analysis. As a result, a wide size range of particles (mainly 500 and 810 nm) with a polydispersity index (PDI) of 0.88 was made, mainly amorphous in structure and as the initial polymer lacking functional groups. The non-toxic nature of these nanoparticles for bacteria was tested by MTT method. The application of this method to the production of NPE is prominent in the toxicity effects of these particles in living organisms, the efficient selection of polyethylene-degrading microorganisms from the environment, and for industrial use to facilitate the degradation process of polyethylene and convert waste into high-value products.
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Abbreviations
- PE:
-
polyethylene
- NPE:
-
nano polyethylene
- LLDPE:
-
linear low density polyethylene
- SEM:
-
scanning electron microscope
- FTIR:
-
Fourier-transform infrared spectroscopy
- DLS:
-
Dynamic Light Scattering
- XRD:
-
X-ray diffraction
- PDI:
-
polydispersity index
- PET:
-
polyethylene terephthalate
- BH:
-
Bushnell Hass
- OD:
-
optical density
- LB:
-
Luria Bertani
- CFU:
-
colony forming unit
- CTAB:
-
Cetyltrimethyl ammonium bromide
References
Arkatkar A, Juwarkar AA, Bhaduri S, Uppara PV, Doble M (2010) Growth of Pseudomonas and Bacillus biofilms on pretreated polypropylene surface. Int Biodeterior Biodegrad 64:530–536. https://doi.org/10.1016/j.ibiod.2010.06.002
Balakrishnan G, Déniel M, Nicolai T, Lagarde F (2019) Towards more realistic reference microplastics and nanoplastics: preparation of polyethylene micro/nanoparticles with a biosurfactant. Environ Sci Nano 6:315–324. https://doi.org/10.1039/c8en01005f
Baudot C, Tan CM, Kong JC (2010) FTIR spectroscopy as a tool for nano-material characterization. Infrared Phys Technol 53:434–438. https://doi.org/10.1016/j.infrared.2010.09.002
Bellasi A, Binda G, Pozzi A, Galafassi S, Volta P, Bettinetti R (2020) Microplastic contamination in freshwater environments: A review, focusing on interactions with sediments and benthic organisms. Environ - MDPI 7. https://doi.org/10.3390/environments7040030
Carvalho PM, Felício MR, Santos NC, Gonçalves S, Domingues MM (2018) Application of light scattering techniques to nanoparticle characterization and development. Front Chem 6:1–17. https://doi.org/10.3389/fchem.2018.00237
Chatterjee J, Haik Y, Chen CJ (2002a) Polyethylene magnetic nanoparticle: A new magnetic material for biomedical applications. J Magn Magn Mater 246:382–391. https://doi.org/10.1016/S0304-8853(02)00110-5
Chatterjee J, Haik Y, Chen CJ (2002b) Synthesis of polyethylene magnetic nanoparticles. J Dispers Sci Technol 23:563–568. https://doi.org/10.1081/DIS-120014024
Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A, Khorasani S, Mozafari MR (2018) Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics 10. https://doi.org/10.3390/pharmaceutics10020057
Danso D, Schmeisser C, Chow J, Zimmermann W, Wei R, Leggewie C, Li X, Hazen T, Streit WR (2018) New insights into the function and global distribution of polyethylene terephthalate (PET)-degrading bacteria and enzymes in marine and terrestrial metagenomes. Appl Environ Microbiol 84. https://doi.org/10.1128/AEM.02773-17
de Vasconcellos SP, Angolini CFF, García INS, Martins Dellagnezze B, da Silva CC, Marsaioli AJ, Neto EV, de Oliveira VM (2010) Screening for hydrocarbon biodegraders in a metagenomic clone library derived from Brazilian petroleum reservoirs. Org. Geochem. 41: 675–681. https://doi.org/10.1016/j.orggeochem.2010.03.014
Ghatge S, Yang Y, Ahn JH, Hur HG (2020) Biodegradation of polyethylene: a brief review. Appl Biol Chem 63. https://doi.org/10.1186/s13765-020-00511-3
Grela E, Kozłowska J, Grabowiecka A (2018) Current methodology of MTT assay in bacteria – A review. Acta Histochem 120:303–311. https://doi.org/10.1016/j.acthis.2018.03.007
Harshvardhan K, Jha B (2013) Biodegradation of low-density polyethylene by marine bacteria from pelagic waters, Arabian Sea, India. Mar Pollut Bull 77:100–106. https://doi.org/10.1016/j.marpolbul.2013.10.025
Kolb L, Monteil V, Thomann R, Mecking S (2005) Aqueous dispersions of extraordinarily small polyethylene nanoparticles. Angew Chemie - Int Ed 44:429–432. https://doi.org/10.1002/anie.200460455
Krishnaswamy K, Orsat V (2017) Sustainable Delivery Systems Through Green Nanotechnology. In: Nano- and Microscale Drug Delivery Systems: Design and Fabrication, pp. 17–32. https://doi.org/10.1016/B978-0-323-52727-9.00002-9
Krueger MC, Harms H, Schlosser D (2015) Prospects for microbiological solutions to environmental pollution with plastics. Appl Microbiol Biotechnol 99:8857–8874. https://doi.org/10.1007/s00253-015-6879-4
Montazer Z, Najafi MBH, Levin DB (2020) Challenges with verifying microbial degradation of polyethylene. Polym (Basel) 12. https://doi.org/10.3390/polym12010123
Mourdikoudis S, Pallares RM, Thanh NTK (2018) Characterization techniques for nanoparticles: Comparison and complementarity upon studying nanoparticle properties. Nanoscale 10:12871–12934. https://doi.org/10.1039/c8nr02278j
Narancic T, O’Connor KE (2017) Microbial biotechnology addressing the plastic waste disaster. Microb Biotechnol 10:1232–1235. https://doi.org/10.1111/1751-7915.12775
Nasir A, Kausar A, Younus A (2015) A Review on Preparation, Properties and Applications of Polymeric Nanoparticle-Based Materials. Polym - Plast Technol Eng 54:325–341. https://doi.org/10.1080/03602559.2014.958780
Prakash T, Taylor TD (2012) Functional assignment of metagenomic data: Challenges and applications. Brief Bioinform 13:711–727. https://doi.org/10.1093/bib/bbs033
Ragaert K, Delva L, Van Geem K (2017) Mechanical and chemical recycling of solid plastic waste. Waste Manag 69:24–58. https://doi.org/10.1016/j.wasman.2017.07.044
Raghavan D, Torma AE (1992) DSC and FTIR characterization of biodegradation of polyethylene. Polym Eng Sci 32:438–442. https://doi.org/10.1002/pen.760320609
Rillig MC (2012) Microplastic in terrestrial ecosystems and the soil? Environ Sci Technol 46:6453–6454. https://doi.org/10.1021/es302011r
Shah AA, Hasan F, Hameed A, Ahmed S (2008) Biological degradation of plastics: A comprehensive review. Biotechnol Adv 26:246–265. https://doi.org/10.1016/j.biotechadv.2007.12.005
Shankar S, Singh S, Mishra A, Sharma M, Shikha (2019) Microbial Degradation of Polyethylene: Recent Progress and Challenges. In: Microbial Metabolism of Xenobiotic Compounds. Microorganisms for Sustainability, Springer Singapore, pp. 245–262. https://doi.org/10.1007/978-981-13-7462-3_12
Shen M, Zhang Y, Zhu Y, Song B, Zeng G, Hu D, Wen X, Ren X (2019) Recent advances in toxicological research of nanoplastics in the environment: A review. Environ Pollut 252:511–521. https://doi.org/10.1016/j.envpol.2019.05.102
Sivan A (2011) New perspectives in plastic biodegradation. Curr Opin Biotechnol 22:422–426. https://doi.org/10.1016/j.copbio.2011.01.013
Soleimani Z, Gharavi S, Soudi M, Moosavi-Nejad Z (2021) A survey of intact low-density polyethylene film biodegradation by terrestrial Actinobacterial species. Int Microbiol 24:65–73. https://doi.org/10.1007/s10123-020-00142-0
Suriya Prabha A, Dorothy R, Jancirani S, Rajendran S, Singh G, Senthil Kumaran S (2020) Recent advances in the study of toxicity of polymer-based nanomaterials. In: Nanotoxicity, INC, pp. 143–165. https://doi.org/10.1016/b978-0-12-819943-5.00007-5
Titus D, James Jebaseelan Samuel E, Roopan SM (2019) Nanoparticle characterization techniques. In: Green Synthesis, Characterization and Applications of Nanoparticles. pp. 303–319. https://doi.org/10.1016/b978-0-08-102579-6.00012-5
Vladár AE, Hodoroaba VD (2020) Characterization of nanoparticles by scanning electron microscopy. In: Characterization of Nanoparticles, pp. 7–27. https://doi.org/10.1016/B978-0-12-814182-3.00002-X
Wei R, Oeser T, Barth M, Weigl N, Lübs A, Schulz-Siegmund M, Hacker MC, Zimmermann W (2014) Turbidimetric analysis of the enzymatic hydrolysis of polyethylene terephthalate nanoparticles. J Mol Catal B Enzym 103:72–78. https://doi.org/10.1016/j.molcatb.2013.08.010
Wierckx N, Prieto MA, Pomposiello P, de Lorenzo V, O’Connor K, Blank LM (2015) Plastic waste as a novel substrate for industrial biotechnology. Microb Biotechnol 8:900–903. https://doi.org/10.1111/1751-7915.12312
Wong SL, Ngadi N, Abdullah TAT (2014) Study on Dissolution of Low Density Polyethylene (LDPE). Appl Mech Mater 695:170–173. https://doi.org/10.4028/www.scientific.net/amm.695.170
Zielinska A, Carreiró F, Oliveira AM, Neves A, Pires B, Nagasamy Venkatesh D, Durazzo A, Lucarini M, Eder P, Silva AM, Santini A, Souto EB (2020) Polymeric Nanoparticles: Production, Characterization, Toxicology and Ecotoxicology. Molecules 25:3731. https://doi.org/10.3390/molecules25163731
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Zokaei, F.H., Gharavi, S., Asgarani, E. et al. A simple method for the production of nano polyethylene particles for rapid assay of polyethylene biodegradation. Biologia 78, 179–186 (2023). https://doi.org/10.1007/s11756-022-01225-1
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DOI: https://doi.org/10.1007/s11756-022-01225-1