Abstract
Lipaphis erysimi (Lipaphis erysimi erysimi and Lipaphis erysimi pseudobrassicae) group, often known as the mustard or turnip aphid, is one of the most devastating specialist aphid of Rapeseed-mustard, causing yield losses of 9 to 96%. This study was carried out with a goal of precisely identifying the dominant specialist aphid of Rapeseed-mustard and to address the issues such as genetic differentiation from different Rapeseed-mustard growing regions covering all the six zones of India. The dendrogram, Principal component analysis (PCA) and phylogenetic relationships revealed that populations of Jorhat (North Eastern India) were highly diverged. Phylogenetic relationship produced by Tamura 3-parameter with Gamma distribution (T92 + G) model using mtCOI demonstrated the presence of L. e. pseudobrassicae in all of the surveyed locations in India including Chintapalli (Andhra Pradesh) a Southern India, where it was reported for the first time. Neutrality tests viz. Tajima and Fisher test supported neutral theory of evolution. Furthermore, our findings in mapping the distribution of L. e. pseudobrassicae in various locations will pave the way for future research and the development of area-wide management tactics for L. e. pseudobrassicae in India.
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Abbreviations
- BIC:
-
Bayesian information criterion
- BLAST:
-
Basic local alignment search tool
- CTAB:
-
Cetyltrimethyl ammonium bromide
- EDTA:
-
Ethylene diamine tetraacetic acid
- EMR:
-
Effective multiplex ratio
- MEGA X:
-
Molecular evolutionary genetic analysis version X
- MI:
-
Marker index
- NCBI:
-
National center for biotechnology information
- PCA:
-
Principal component analysis
- PCR:
-
Polymerase chain reaction
- PIC:
-
Polymorphism information content
- PPB:
-
Percentage of polymorphic bands
- RAPD:
-
Random amplified polymorphic DNA
- RARS:
-
Regional agricultural research station
- RP:
-
Resolving power
- SNPs:
-
Single nucleotide polymorphisms
- SSR:
-
Simple sequence repeats
- T92 + G:
-
Tamura 3-parameter with Gamma distribution
- TAE:
-
Tris acetate EDTA
- UPGMA:
-
Unweighted pair-group method with arithmetic averages
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Acknowledgements
The authors are grateful to Department of Entomology and Department of Genetics and Plant Breeding of Dr. Rajendra Prasad Central Agricultural University for their unwavering support throughout the research. This research is part of Somala Karthik PhD thesis at the RPCAU in Bihar.
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• Somala Karthik: Planned and executed the research
• Gummudala Yashaswini: Writing assistance, proof reading of the article
• Udayan Mukherjee: Proof reading of the article
• Beerelli Deepak Reddy: Sample collection and proof reading of the article
• Gurram Mallikarjun: Sample collection and proof reading of the article
• Alagesan Keerthana: Sample collection and DNA extraction
• Morthala Shankara Sai Reddy: Supervised and guided the researcher
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Karthik, S., Yashaswini, G., Mukherjee, U. et al. Phylogenetic divergence of Lipaphis erysimi pseudobrassicae (Aphididae: Homoptera): a dominant specialist aphid on Rapeseed-mustard, India. Biologia 77, 3603–3614 (2022). https://doi.org/10.1007/s11756-022-01213-5
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DOI: https://doi.org/10.1007/s11756-022-01213-5