Abstract
An efficient genotype-dependent high frequency plant regeneration system was developed for finger millet genotype GN-4 (Gujarat Nagli-4). For callus induction, five different concentrations of 2,4-D (0.5, 1.0, 2.0, 3.0 and 4.0 mg L−1) were used to culture mature seeds and observed notable percentage of callus induction 94.00%; fresh and dry weight (0.903 g and 0.093 g) on Murashige and Skoog (MS) medium containing 2.0 mg L−1 2,4-D. Maximum callus proliferation (92.80%) with good quality of callus were observed on MS basal medium having 2.0 mg L−1 2,4-D + 0.5 mg L−1 BAP + 0.5 mg L−1 kinetin. On transferring callus for shoot generation, most effective regeneration of shoots (88.30%) with higher number of shoots per explant (8.00) and maximum shoot length (6.90 cm) were observed on MS + 1.0 mg L−1 BAP + 1.0 mg L−1 kinetin. For rooting, regenerated shoots were cultured to full-MS and half-MS media enriched with different levels of IBA and NAA and recorded remarkable early rooting response (71.67%) on half-MS medium + 0.1 mg L−1 IBA. These healthy rooted plants were transferred to pots containing sterilized potting mixture consisting vermicompost: soil: coco peat (1:1:1) for hardening and successfully acclimatized in the field conditions. Genetic stability of regenerated plantlets was confirmed by randomly amplified polymorphic DNA (RAPD) with monomorphic bands during different stages of plant tissue culture system and proved no variation. Furthermore, minerals (P, Mg, Ca and Fe) study based on inductively coupled plasma mass spectrometry (ICP-MS) determined higher content of P, Ca and Fe in seeds of regenerated plantlets as compared to mother plant.
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Abbreviations
- ANOVA:
-
Analysis of Variance
- BAP:
-
6-Benzyl Amino Purine
- GN-4:
-
Gujarat Nagli-4
- IBA:
-
Indole-3-butyric acid
- ICP-MS:
-
Inductively coupled plasma-mass spectrometry
- MS:
-
Murashige and Skoog
- NAA:
-
α-Naphthaleneacetic acid
- RAPD:
-
Randomly amplified polymorphic DNA
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
References
Amadou I, Gounga ME, Le GW (2013) Millets: Nutritional composition, some health benefits and processing-A review. Emirates J Food Agric :501-508. https://doi.org/10.9755/ejfa.v25i7.12045
Anonymous (2018) Improved varieties of Small Millets released by HMRS, NAU, Waghai for Gujarat state. https://nau.in/assets/uploads/7cd3e-hmrsw_1542905486_109technology-developed.pdf. Accessed 28.01.2019
Ashwini AM, Ramakrishnaiah H, Manohar SH, Majumdar M (2015) An efficient multiple shoot induction and genetic fidelity assessment of Exacum bicolor Roxb., an endemic and endangered medicinal plant. In Vitro Cell Dev Biol Plant 51(6):659–668. https://doi.org/10.1007/s11627-015-9726-5
Babu TK, Thakur RP, Upadhyaya HD, Reddy PN, Sharma R, Girish AG, Sarma NDRK (2013) Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm. Eur J Plant Pathol 135(2):299–311. https://doi.org/10.1007/s10658-012-0086-2
Babu GA, Vinoth A, Ravindhran R (2018) Direct shoot regeneration and genetic fidelity analysis in finger millet using ISSR markers. Plant Cell Tissue Organ Cult 132(1):157–164. https://doi.org/10.1007/s11240-017-1319-z
Badigannavar A, Ganapathi TR (2018) Genetic variability for mineral nutrients in indigenous germplasm lines of finger millet (Eleusine coracana Gaertn.). J Cereal Sci 84:1–6. https://doi.org/10.1016/j.jcs.2018.09.014
Chandra D, Chandra S, Sharma AK (2016) Review of Finger millet (Eleusine coracana (L.) Gaertn): a power house of health benefiting nutrients. Food Sci Hum Wellness 5(3):149–155. https://doi.org/10.1016/j.fshw.2016.05.004
Chandra AK, Chandora R, Sood S, Malhotra N (2021) Global production, demand, and supply. In Millets and Pseudo Cereals (pp. 7–18). Woodhead Publishing. https://doi.org/10.1016/B978-0-12-820089-6.00002-1
Chaudhari PP, Patel DA, Saravaiya SN, Patel MC (2012) GN-5: A new high yielding white grain finger millet variety. Int J Plant Sci 7(1):51–53. http://researchjournal.co.in/online/IJPS/IJPS%207(1)/7_A-51-53.pdf. Accessed 12.11.2018
David BM, Afolayan M, Odukomaiya D, Ikokoh P, Orishadipe A (2014) Proximate composition, mineral and phytochemical constituents of Eleusine coracana (Finger millet). Int J Adv Chem 2(2):171–174. https://doi.org/10.14419/ijac.v2i2.3496
Devi PB, Vijayabharathi R, Sathyabama S, Malleshi NG, Priyadarisini VB (2014) Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: a review. J Food Sci Technol 51(6):1021–1040. https://doi.org/10.1007/s13197-011-0584-9
Dosad S, Chawla HS (2015) In vitro plant regeneration from mature seeds of finger millet (Eleusine coracana) through somatic embryogenesis. Indian J Plant Physiol 20(4):360–367. https://doi.org/10.1007/s40502-015-0191-2
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Food and Agriculture Organization of the United Nations (FAOSTAT) (2018) http://faostat.fao.org/ accessed 21.12.2018.
Hansen TH, Laursen KH, Persson DP, Pedas P, Husted S, Schjoerring JK (2009) Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis. Plant Methods 5(1):1–11. https://doi.org/10.1186/1746-4811-5-12
Hulse JH, Laing EM, Pearson OE (1980) Sorghum and the millets: their composition and nutritive value. Academic press, New York
Ikeuchi M, Sugimoto K, Iwase A (2013) Plant callus: mechanisms of induction and repression. Plant Cell 25(9):3159–3173. https://doi.org/10.1105/tpc.113.116053
Jajda HM, Patel KG, Patel SR, Solanki VH, Patel KN, Singh S (2013) Comparative efficacy of two standard methods for determination of iron and zinc in fruits, pulses and cereals. J Food Sci Technol 52(2):1096–1102. https://doi.org/10.1007/s13197-013-1088-6
Joshi G, Shukla A, Shukla A (2011) Synergistic response of auxin and ethylene on physiology of Jatropha curcas L. Braz J Plant Physiol 23:66–77. https://doi.org/10.1590/S1677-04202011000100009
Joshi BK, Joshi D, Ghimire SK (2020) Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers. Nepal J Biotechnol 8(1):1–11. https://doi.org/10.3126/njb.v8i1.30204
Kansara RV, Jha S, Jha SK, Mahatma MK (2013) An efficient protocol for in vitro mass propagation of fusarium wilt resistant castor (Ricinus communis L.) parental line SKP-84 through apical meristem. Bioscan 8(2):403
Kashyap A, Penak SM, Saha A, Singh BR (2018) In vitro plant development of Eleusine coracana via indirect organogenesis and somatic embryogenesis using mature seeds as explants. Curr Sci 115(1):91–98. https://doi.org/10.18520/cs/v115/i1/91-98
Malik SI, Rashid H, Yasmin TAYYABA, Minhas NM (2004) Effect of 2,4-dichlorophenoxyacetic acid on callus induction from mature wheat (Triticum aestivum L.) seeds. Int J Agric Biol 6(1):156–159
Mallikharjun DR, Hiremath SC, Rao SR, Kumar A, Sheelavanthmath SS (2005) Genome interrelationship in the genus Eleusine (Poaceae) as revealed through heteroploid crosses. Caryologia 58(4):300–307. https://doi.org/10.1080/00087114.2005.10589467
Mundada PS, Umdale SD, Nikam TD, Ahire ML (2019) Genetic diversity using RAPD markers, mineral composition and their correlation in selected local landraces of finger millet [Eleusine coracana (L.) Gaertn.]. Vegetos 32(1):1–10. https://doi.org/10.1007/s42535-019-00001-y
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Owheruo JO, Ifesan BO, Kolawole AO (2019) Physicochemical properties of malted finger millet (Eleusine coracana) and pearl millet (Pennisetum glaucum). Food Sci Nutr 7(2):476–482. https://doi.org/10.1002/fsn3.816
Pande A, Dosad S, Chawla HS, Arora S (2014) In-vitro organogenesis and plant regeneration from seed-derived callus cultures of finger millet (Eleusine coracana). Braz J Bot 38(1):19–23. https://doi.org/10.1007/s40415-014-0102-1
Pandey S, Patel P, Prasad A, Sawant SV, Misra P (2020) Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal a commercially important medicinal plant. In Vitro Cell Dev Biol Plant 56(4):538–547. https://doi.org/10.1007/s11627-020-10073-0
Panse VG, Sukhatme PV (1985) Statistical analysis for agricultural workers. ICAR, New Delhi
Prabhu KS, Das AB, Dikshit N (2018) Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers. Zeitschrift für Naturforschung C 73(5–6):165–176. https://doi.org/10.1515/znc-2017-0182
Sakamma S, Umesh KB, Girish MR, Ravi SC, Satishkumar M, Bellundagi V (2018) Finger millet (Eleusine coracana L. Gaertn.) production system: status, potential, constraints and implications for improving small farmer’s welfare. J Agric Sci 10:162–179. https://doi.org/10.5539/jas.v10n1p162
Satish L, Ceasar SA, Shilpha J, Rency AS, Rathinapriya P, Ramesh M (2015) Direct plant regeneration from in vitro-derived shoot apical meristems of finger millet (Eleusine coracana (L.) Gaertn.). In Vitro Cell Dev Biol Plant 51(2):192–200. https://doi.org/10.1007/s11627-015-9672-2
Sharma A, Kumar N, Mishra IG (2018) Role of molecular marker in the genetic improvement of the medicinal and aromatic plants. In Biotechnological Approaches for Medicinal and Aromatic Plants. Springer, 557–567. https://doi.org/10.1007/978-981-13-0535-1_25
Sompornpailin K, Khunchuay C (2016) Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (’Vetiveria zizanioides’ L. Nash). Aust J Crop Sci 10(5):726–73. https://doi.org/10.21475/ajcs.2016.10.05.p7439
Williams GKJ, Kubellik RA, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535. https://doi.org/10.1093/nar/18.22.6531
Acknowledgements
Authors are thankful to Hill Millet Research Station, Waghai Dist. Dang, NAU, Navsari, Gujarat, India for providing germplasm to carry out the experiment. We are also thankful to Food Quality Testing Laboratory, Navsari Agricultural University, Navsari, Gujarat, India for providing facilities of analytical instrument.
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RVK, VHS and RMP performed the research work. RVK, VHS, RMP and VS were contributed to all data collection during experiments. RVK, KDG and VM analyzed data and prepared primary draft of manuscript. All authors reviewed and approved the final version of the manuscript.
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Kansara, R.V., Solanki, V.H., Srivashtav, V.S. et al. In vitro regeneration through indirect organogenesis, genetic uniformity analysis and mineral content determination of finger millet (Eleusine coracana L.). Biologia 78, 67–77 (2023). https://doi.org/10.1007/s11756-022-01179-4
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DOI: https://doi.org/10.1007/s11756-022-01179-4