Abstract
The present study is aimed at isolating a heavy metal and non-metallic pollutants tolerant microorganism from wastewater samples which has the highest capability to remove toxicants from contaminated water bodies. From the wastewater samples of Tolly Canal, 35 bacterial, 10 yeast and 5 filamentous fungal strains were isolated and tested for their toxicant tolerance capacities. The obtained isolates were subjected to gradually increasing concentration of toxic metal salts Pb(CH3COO)2.3H2O, NiSO4.6H2O, CuSO4.5H2O, CdCl2.H2O, NaF, NaAsO2. All the isolated strains reached the minimal inhibitory concentration much earlier than a yeast strain named as JIC. The strain was proved to be the most toxicant tolerant and also showed efficient toxicant removal capacity by employing 10 ppm of each of the pollutants. It showed removal capacities of Cd (II) (43.60%), Pb (II) (38.20%), Cu (II) (22.60%), As (III) (31.20%), F−(27.60%) and Ni (II) (24.80%). The isolated strain has been undergone some biochemical and phylogenetic analyses for identification and subsequently found to be a strain of Candida tropicalis. The Internal Transcribed Spacer (ITS) 1–4 RNA gene sequence obtained was deposited to National Center for Biotechnology Information (NCBI) and finally the accession number has been obtained as Candida tropicalis isolate JIC MZ798901.
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Abbreviations
- EDAX:
-
Energy-dispersive X-ray
- ITS:
-
Internal Transcribed Spacer
- FT-IR:
-
Fourier Transform Infrared
- SEM:
-
Scanning Electron Microscope
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Acknowledgements
The authors would like to thank CRNN, University of Calcutta, Bose Institute, Indian Association for the Cultivation Of Science, Jadavpur, Kolkata, S.N. Bose National Centre for Basic Sciences and CSIR-Indian Institute Of Chemical Biology (IICB) for providing necessary technical supports for analysis of samples and to Dr. Smaranika Pattanaik (DSc.), Assistant Professor, Department of Biotechnology and Bioinformatics, Medical Microbiotechnology Laboratory, Sambalpur University, Odisha, India, our research collaborator for her kind assistance in testing the virulence of the isolated Candida tropicalis isolate JIC MZ798901.
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The work was financially supported by the Council of Scientific & Industrial Research, Human Resource Development Group (Extra Mural Research Division), CSIR Complex, Library Avenue, Pusa, New Delhi 110012, India, Award Letter Number:08/463(0001)/2019-EMR-1.
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The experiment was conceptualized and designed by Dr. Subhadeep Ganguly. The experiment was conducted by Kaustav Bhattacharyya, Debrup Sen and Basab B. Dey. The molecular phylogenetic analysis and tree construction were done by Dr. Arijit De. FTIR-ATR data have been explained by Dr. Neelanjan Bhattacharjee. The concept was theoretically enriched by Dr. Asit Baran Biswas.
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Bhattacharyya, K., Sen, D., Dey, B.B. et al. Isolation and characterization of heavy metals and non-metallic pollutant-tolerant microorganism from wastewater of Tollygunge Canal (Kolkata) West Bengal, India. Biologia 77, 2359–2369 (2022). https://doi.org/10.1007/s11756-022-01086-8
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DOI: https://doi.org/10.1007/s11756-022-01086-8