Abstract
Pepper commercial hybrids, produced by an economical and efficient cytoplasmic male sterility (CMS)/restorer-of-fertility (Rf) system, have been widely used in production because of their strong heterosis. However, the restorer resources of pepper are rare in nature, which makes it necessary to transfer the Rf gene from restorer lines to maintainer lines to produce hybrid F1 seeds. Here, we backcrossed a core maintainer line ‘247’ (receptor) with a three-line hybrid pepper ‘17–120’ (donor) to transfer the Rf gene into the maintainer line. Two molecular markers, CRF and SCD06-17, linked to the Rf gene were used to screen individual plants with the Rf gene (foreground selection) and 31 SSR markers were used in BC1F1 and BC2F1 generations to screen individuals with the highest similarity to the genetic background of the recurrent parent (background selection). Restorer line ‘247R’ was acquired in four generations of breeding and did not differ significantly with the recurrent parent between the main agronomic traits. The scheme used in this study accelerated the fertility conversion process between maintainer lines and restorer lines, which lays a foundation for the production of hybrid seeds by maintainer lines.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- CAPS:
-
cleaved amplified polymorphic sequence
- CMS:
-
cytoplasmic male sterility
- CTAB:
-
cetyltrimethylammonium bromide
- GMS:
-
genic male sterility
- MAB:
-
marker-assisted backcrossing
- MAS:
-
marker-assisted selection
- PCR:
-
Polymerase chain reaction
- PPR:
-
pentatricopeptide repeat
- RAPD:
-
randomly amplified polymorphic DNA
- Rf:
-
restorer-of-fertility
- SCAR:
-
sequence characterized amplified region
- SSR:
-
simple sequence repeats
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Acknowledgements
This research was financially supported by the scientific and technological project of Henan Province (182102110100, 1921011045). The authors thank Dr. Craig A.L. (Texas A&M University) for his critical reading of the manuscript.
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Ren FS and Jiao YS conceived the study and designed the research. Yang HF, Jiao YS, Zhang RP, and Dong YQ conducted the experiments and the data analysis. Ren FS and Dong YQ wrote the manuscript. Guo ZW, Chen HF, Liu HJ, and Sun Q participated in the measurement of pollen fertility and agronomic and morphological characters of the recurrent parent XK15-247 and its progenies. Yang HF and Tan XF provided suggestions on the data analysis and revised the manuscript. All the authors read and approved the final version of the manuscript.
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Hai-Feng Yang contributed equally to this work.
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Ren, FS., Yang, HF., Jiao, YS. et al. Fertility conversion between cytoplasmic maintainer lines and restorer lines through molecular marker-assisted selection in pepper (Capsicum annuum L.). Biologia 77, 2351–2358 (2022). https://doi.org/10.1007/s11756-022-01080-0
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DOI: https://doi.org/10.1007/s11756-022-01080-0