Abstract
In the present study, we investigated the effects of exogenous silicon (Si) treatment on seed germination, embryo viability, seedling growth, antioxidant molecules and osmotic regulation in fenugreek under salt stress. The seeds were germinated in Petri dishes at 25 ± 1 °C for 8 days under 0 mM (control) versus 200 mM NaCl (salt stress) with or without 3 mM Si treatment. The obtained results showed that salt stress significantly reduced final germination percentage, germination speed, velocity index, germination energy, peak value, germination value and vitality index, but increased the mean germination time of fenugreek seeds. Salinity also significantly impaired seedling length and fresh weight. However, Si supplementation contracted the negative impacts of salinity and significantly increased all of the studied germination traits except of mean germination time. Additionally, Si alleviated the negative effects of salt on embryo viability and improved its ability to mobilize sugar and protein reserves. Furthermore, 200 mM NaCl increased Na+ content and induced the oxidative stress reflected by high malonyldialdehyde content, reactive oxygen species accumulation and electrolyte leakage percentage. However, salt-mediated oxidative stress was alleviated by Si treatment through a significant decrease of Na+ content and an increase of endogenous Si content, which correlated with a significant induction of enzymatic and non-enzymatic molecules with antioxidant function. Altogether, these findings suggested that, the exogenous Si treatment could be a potential method to enhance salt tolerance of fenugreek during seed germination.
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References
Abdul Jaleel C, Lakshmanan GMA, Gomathinayagam M, Panneerselvam R (2008) Triadimefon induced salt stress tolerance in Withania somnifera and its relationship to antioxidant defense system. S Afr J Bot 74(1):126–132. https://doi.org/10.1016/J.SAJB.2007.10.003
Aghaei K, Komatsu S (2013) Crop and medicinal plants proteomics in response to salt stress. Front in Plant Sci 4:1–9. https://doi.org/10.3389/fpls.2013.00008
Almutairi MZ (2016) Effect of nano-silicon application on the expression of salt tolerance genes in germinating Tomato (Solanum lycopersicum L.) seedlings under salt stress. Plant Omics 9(1):106–114. https://doi.org/10.3316/informit.888088806058398
Alrumaihi FA, Khan MA, Allemailem KS, Alsahli MA, Almatroudi A, Younus H, Alsuhaiban SA, Algahtani M, Khan A (2021) Methanolic Fenugreek seed extract induces p53-dependent mitotic catastrophe in breast cancer cells, leading to apoptosis. J Inflamm Res 14:1511–1535. https://doi.org/10.2147/JIR.S300025
Al-Saeedi AH, Ragab El-Ramady H, Alshaal T, El-Garawany MM, Elhawat N, Almohsen M (2017) Engineered silica nanoparticles alleviate the detrimental effects of Na+ stress on germination and growth of Common bean (Phaseolus vulgaris). Environ Sci Pollut Res 1–12. https://doi.org/10.1007/s11356-017-9847-y
Amraee L, Rahmani F, Abdollahi Mandoulakani B (2020) Exogenous application of 24-epibrassinosteroid mitigates NaCl toxicity in Flax by modifying free amino acids profile and antioxidant defence system. Funct Plant Biol 47(6):575. https://doi.org/10.1071/FP19191
Basch E, Ulbricht C, Kuo G, Szapary P (2003) Therapeutic applications of Fenugreek. Altern Med Rev 8(1):20–27
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 391:205–207. https://doi.org/10.1007/BF00018060
Ben Taârit M, Msaada K, Hosni K, Marzouk B (2012) Fatty acids, phenolic changes and antioxidant activity of Clary sage (Salvia sclarea L.) rosette leaves grown under saline conditions. Ind Crops Prod 38(1):58–63. https://doi.org/10.1016/J.INDCROP.2012.01.002
Benayad Z, Gómez-Cordovés C, Es-Safi NE (2014) Identification and quantification of flavonoid glycosides from Fenugreek (Trigonella foenum-graecum) germinated seeds by LC–DAD–ESI/MS analysis. J Food Compost Anal 35(1):21–29. https://doi.org/10.1016/J.JFCA.2014.04.002
Benmahioul B, Daguin F, Kaid-Harche M (2009) Effects of salt stress on germination and in vitro growth of Pistachio (Pistacia vera L.). C R Biol 332(8):752–758. https://doi.org/10.1016/j.crvi.2009.03.008
Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions. Anal Biochem 161(2):559–566. https://doi.org/10.1016/0003-2697(87)90489-1
Bianco C, Defez R (2009) Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain. J Exp Bot 60(11):3097–3107. https://doi.org/10.1093/JXB/ERP140
Biju S, Fuentes S, Gupta D (2017) Silicon improves seed germination and alleviates drought stress in Lentil crops by regulating osmolytes, hydrolytic enzymes and antioxidant defense system. Plant Physiol Biochem 119:250–264. https://doi.org/10.1016/J.PLAPHY.2017.09.001
Bonilla I, El-Hamdaoui A, Bolaños L (2004) Boron and calcium increase Pisum sativum seed germination and seedling development under salt stress. Plant Soil 267(1):97–107. https://doi.org/10.1007/S11104-005-4689-7
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Brennan T, Frenkel C (1977) Involvement of hydrogen peroxide in the regulation of senescence in Pear1. Plant Physiol 59:411–416
Calone R, Sanoubar R, Noli E, Barbanti L (2020) Assessing Salicornia europaea tolerance to salinity at seed germination stage. Agric 10(2):29. https://doi.org/10.3390/AGRICULTURE10020029
Chang CC, Yang MH, Wen HM, Chern J.C (2002) Estimation of total flavonoid content in Propolis by two complementary colometric methods. J Food Drug Anal 10(3):178–182. https://doi.org/10.38212/2224-6614.2748
Cha-Um S, Kirdmanee C (2010) Effect of glycinebetaine on proline, water use, and photosynthetic efficiencies, and growth of Rice seedlings under salt stress. Turk J Agric for 34(6):517–527. https://doi.org/10.3906/tar-0906-34
Czabator FJ (1962) Germination value: An index combining speed and completeness of pine seed germination. For Sci 8(4):386–396. https://doi.org/10.1093/FORESTSCIENCE/8.4.386
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (2002) Colorimetric method for determination of sugars and related substances. Microb Cell Fact 28(3):350–356. https://doi.org/10.1021/AC60111A017
El Moukhtari A, Cabassa-Hourton C, Farissi M, Savouré A (2020) How does proline treatment promote salt stress tolerance during crop plant development? Front Plant Sci 11:1–16. https://doi.org/10.3389/FPLS.2020.01127
El Moukhtari A, Carol P, Mouradi M, Savouré A, Farissi M (2021) Silicon improves physiological, biochemical, and morphological adaptations of alfalfa (Medicago sativa L.) during salinity stress. Symbiosis 1:1–20. https://doi.org/10.1007/S13199-021-00814-Z
Farissi M, Bouizgaren A, Faghire M, Bargaz A, Ghoulam C (2011) Agro-physiological responses of Moroccan alfalfa (Medicago sativa L.) populations to salt stress during germination and early seedling stages. Seed Sci Technol 39(2):389–401
Ghoulam C, Foursy A, Fares K (2002) Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five Sugar beet cultivars. Environ Exp Bot 47(1):39–50
Gou T, Chen X, Han R, Liu J, Zhu Y, Gong H (2020) Silicon can improve seed germination and ameliorate oxidative damage of bud seedlings in Cucumber under salt stress. Acta Physiol Plant 42(1):1–11. https://doi.org/10.1007/s11738-019-3007-6
Grieve CM, Grattan SR (1983) Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70(2):303–307. https://doi.org/10.1007/BF02374789
Haddad PS, Depot M, Settaf A, Chabli A, Cherrah Y (2003) Comparative study on the medicinal plants most recommended by traditional practitioners in Morocco and Canada. J Herbs Spices Med Plants 10(3):25–45. https://doi.org/10.1300/J044V10N03_04
Haghighi M, Afifipour Z, Mozafarian M (2012) The Effect of n-Si on Tomato seed germination under salinity levels. J Biol Environ Sci 6(16):87–90
Hassanvand F, Rezaei Nejad A, Fanourakis D (2019) Morphological and physiological components mediating the silicon-induced enhancement of Geranium essential oil yield under saline conditions. Ind Crops Prod 134:19–25. https://doi.org/10.1016/j.indcrop.2019.03.049
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hojjat SS, Kamyab M (2017) The effect of silver nanoparticle on Fenugreek seed germination under salinity levels. Russ Agric Sci 43(1):61–65. https://doi.org/10.3103/s1068367417010189
Hori K, Wada A, Shibuta T (1997) Changes in phenoloxidase activities of the galls on leaves of Ulmus davidana formed by Tetraneura fusiformis (homoptera: eriosomatidae). Appl Entomol Zool 32(2):365–371. https://doi.org/10.1303/aez.32.365
Hosein M, Keshavarzi B (2012) The effect of different NaCl concentration on germination and early seedling growth of Artemisia annua L. Int J Agric 2(3):135–140. http://www.ecisi.com
Hozzein WN, Saleh AM, Habeeb TH, Wadaan MAM, AbdElgawad H (2020) CO2 treatment improves the hypocholesterolemic and antioxidant properties of Fenugreek seeds. Food Chem 308:1–8. https://doi.org/10.1016/J.FOODCHEM.2019.125661
Hua-long L, Han-jing S, Jing-guo W, Yang L, De-tang Z, Hong-wei Z (2014) Effect of seed soaking with exogenous proline on seed germination of Rice under salt stress. J Northeast Agric Univ 21(3):1–6. https://doi.org/10.1016/S1006-8104(14)60062-3
İbrahimova U, Kumari P, Yadav S, Rastogi A, Antala M, Suleymanova Z, Zivcak M, Tahjib-Ul-Arif M, Hussain S, Abdelhamid M et al (2021) Progress in understanding salt stress response in plants using biotechnological tools. J Biotechnol 329:180–191. https://doi.org/10.1016/J.JBIOTEC.2021.02.007
Ivani R, Sanaei Nejad SH, Ghahraman B, Astaraei AR, Feizi H (2018) Role of bulk and Nanosized SiO2 to overcome salt stress during Fenugreek germination (Trigonella foenum- graceum L.). Plant Signal Behavr 13(7):1–6. https://doi.org/10.1080/15592324.2015.1044190
Jaleel CA, Gopi R, Kishorekumar A, Manivannan P, Sankar B, Panneerselvam R (2007) Interactive effects of triadimefon and salt stress on antioxidative status and ajmalicine accumulation in Catharanthus roseus. Acta Physiol Plant 30(3):287–292. https://doi.org/10.1007/S11738-007-0119-1
James JJ, Devi VM (2021) Efficacy of some traditional Indian spices for treating diabetes. Int J Res Sci Eng 9(3):46–53
Khan MA, Ungar IA (1984) The effect of salinity and temperature on the germination of polymorphic seeds and growth of Atriplex triangularis willd. Am J Bot 71(4):481–489. https://doi.org/10.2307/2443323
Khorshidian N, Yousefi Asli M, Arab M, Adeli Mirzaie A, Mortazavian AM (2016) Fenugreek: Potential applications as a functional food and nutraceutical. Nutr Food Sci Res 3(1):5–16. https://doi.org/10.18869/ACADPUB.NFSR.3.1.5
Khoshsokhan F, Babalar M, Chaghazardi HR, Fatahi Moghadam MR (2012) Effect of salinity and drought stress on germination in thyme effect of salinity and drought stress on germination indices of two Thymus species. Cercet Agron Mold 1(149):27–35. https://doi.org/10.2478/v10298-012-0003-z
Kumar D, Yusuf M, Singh P, Sardar M, Sarin N (2014) Histochemical detection of superoxide and H2O2 accumulation in Brassica juncea seedlings. Bio-Protocol 4(8):1–4. https://doi.org/10.21769/BIOPROTOC.1108
Laghmouchi Y, Belmehdi O, Bouyahya A, Skali Senhaji N, Abrini J (2017) Effect of temperature, salt stress and pH on seed germination of medicinal plant Origanum compactum. Biocatal Agric Biotechnol 10:156–160. https://doi.org/10.1016/j.bcab.2017.03.002
Li J, Zhao C, Zhang M, Yuan F, Chen M (2019) Exogenous melatonin improves seed germination in Limonium bicolor under salt stress. Plant Signal Behav 14(11):1–10. https://doi.org/10.1080/15592324.2019.1659705
Liu N, Jørgensen U, Lærke PE (2013) Quality determination of biomass for combustion: A new high-throughput microwave digestion method prior to elemental analysis by Inductively Coupled Plasma-Optical Emission Spectroscopy. Energy Fuels 27(12):7485–7488. https://doi.org/10.1021/EF4016747
Liu J, Li L, Yuan F, Chen M (2019) Exogenous salicylic acid improves the germination of Limonium bicolor seeds under salt stress. Plant Signal Behav 14(10):1–8. https://doi.org/10.1080/15592324.2019.1644595
Luo X, Dai Y, Zheng C, Yang Y, Chen W, Wang Q, Chandrasekaran U, Du J, Liu W, Shu K (2021) The ABI4-RbohD/VTC2 regulatory module promotes reactive oxygen species (ROS) accumulation to decrease seed germination under salinity stress. New Phytol 229(2):950–962. https://doi.org/10.1111/NPH.16921
Mahmoudi H, Ben Salah I, Zaouali W, Hamrouni L, Gruber M, Ouerghi Z, Hosni K (2019) Priming-induced changes in germination, morpho-physiological and leaf biochemical responses of Fenugreek (Trigonella foenum-graecum) under salt stress. Plant Biosyst 154(5)/601–614. https://doi.org/10.1080/11263504.2019.1651785
Moradi Dezfuli P, Sharif-zadeh F, Janmohammadi M (2008) Influence of priming techniques on seed germination behavior of Maize inbred lines (Zea mays L.). J Agric Biol Sci 3(3):22–25. www.arpnjournals.com
Peng Z, He S, Sun J, Pan Z, Gong W, Lu Y, Du X (2016) Na+ compartmentalization related to salinity stress tolerance in Upland cotton (Gossypium hirsutum) seedlings. Sci Rep 6(1):1–14. https://doi.org/10.1038/srep34548
Perveen S, Shahbaz M, Ashraf M (2011) Modulation in activities of antioxidant enzymes in salt stressed and non-stressed Wheat (Triticum aestivum L.) plants raised from seed trearted with triacontanol. Pak J Bot 43(5):2463–2468
Robatjazi R, Roshandel P, Hooshmand D (2020) Benefits of Silicon nutrition on growth, physiological and phytochemical attributes of Basil upon salinity stress. Int J Hortic Sci Technol 7(1):37–50. https://doi.org/10.22059/ijhst.2020.288551.318
Salahuddin M, Nawaz F, Shahbaz M, Naeem M, Zulfiqar B, Shabbir RN, Hussain RA (2017) Effect of exogenous nitric oxide (NO) supply on germination and seedling growth of Mungbean (cv. Nm-54) under salinity stress. Legume Res 40(5):846–852. https://doi.org/10.18805/lr.v0i0.8399
Sebei K, Debez A, Herchi W, Boukhchina S, Kallel H (2007) Germination kinetics and seed reserve mobilization in two Flax (Linum usitatissimum L.) cultivars under moderate salt stress. J Plant Biol 50(4):447–454. https://doi.org/10.1007/BF03030681
Sekmen AH, Turkan I, Tanyolac ZO, Ozfidan C, Dinc A (2012) Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark. Environ Exp Bot 7:63–76. https://doi.org/10.1016/J.ENVEXPBOT.2011.10.012
Shi Y, Zhang Y, Yao H, Wu J, Sun H, Gong H (2014) Silicon improves seed germination and alleviates oxidative stress of bud seedlings in Tomato under water deficit stress. Plant Physiol Biochem 78:27–36. https://doi.org/10.1016/J.PLAPHY.2014.02.009
Sidari M, Santonoceto C, Anastasi U, Preiti G, Muscolo A (2008) Variations in four genotypes of Lentil under NaCl-salinity stress. Am J Agric Biol Sci 3(1):410–416. https://doi.org/10.3844/AJABSSP.2008.410.416
Singh B, Kaur R, Singh K (2008) Characterization of Rhizobium strain isolated from the roots of Trigonella foenumgraecum (Fenugreek). Afr J Biotechnol 7(20):3671–3676. http://www.academicjournals.org/AJB
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16(3):144–158
Sun Y, Xu J, Miao X, Lin X, Liu W, Ren H (2021) Effects of exogenous silicon on Maize seed germination and seedling growth. Sci Rep 11(1):1–13. https://doi.org/10.1038/s41598-020-79723-y
Tang X, Mu X, Shao H, Wang H, Brestic M (2015) Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology. Crit Rev Biotechnol 35(4):425–437. https://doi.org/10.3109/07388551.2014.889080
Thakur M, Sharma AD (2005) Salt-stress-induced proline accumulation in germinating embryos: Evidence suggesting a role of proline in seed germination. J Arid Environ 62(3):517–523. https://doi.org/10.1016/J.JARIDENV.2005.01.005
Verma P, Majee M (2013) Seed germination and viability test in Tetrazolium (TZ) Assay. Bio-Protocol 3(17):1–4. https://doi.org/10.21769/BIOPROTOC.884
Wang XD, Chao OY, Fan ZR, Gao S, Tang L (2010) Effects of exogenous silicon on seed germination and antioxidant enzyme activities of Momordica charantia under salt stress. J Anim Plant Sci 6:700–708. http://www.biosciences.elewa.org/JAPS;ISSN2071-7024
Yan GC, Nikolic M, Ye MJ, Xiao ZX, Liang YC (2018) Silicon acquisition and accumulation in plant and its significance for agriculture. J Integr Agric 17(10):2138–2150. https://doi.org/10.1016/S2095-3119(18)62037-4
Zhang XH, Zhou D, Cui JJ, Ma HL, Lang DY, Wu XL, Wang ZS, Qiu HY, Li M (2015) Effect of silicon on seed germination and the physiological characteristics of Glycyrrhiza uralensis under different levels of salinity. J Hortic Sci Biotechnol 90(4):439–443. https://doi.org/10.1080/14620316.2015.11513207
Zhang W, Xie Z, Wang L, Li M, Lang D, Zhang X (2017) Silicon alleviates salt and drought stress of Glycyrrhiza uralensis seedling by altering antioxidant metabolism and osmotic adjustment. J Plant Res 130(3):611–624. https://doi.org/10.1007/S10265-017-0927-3
Zhang Z, Fan J, Wu J, Zhang L, Wang J, Zhang B, Wang-Pruski G (2020) Alleviating effect of silicon on Melon seed germination under autotoxicity stress. Ecotoxicol Environ Saf 188:1–9. https://doi.org/10.1016/J.ECOENV.2019.109901
Zrig A, Ferreira JFS, Hamouda F, Tounekti T, Selim S, Al Jaouni S, Khemira H, Abdelgawad H (2019) The impact of foliar fertilizers on growth and biochemical responses of Thymus vulgaris to salinity stress. Arid Land Res Manag 33(3):297–320. https://doi.org/10.1080/15324982.2018.1551817
Acknowledgements
Authors would like to knowledge the Research and Analysis Center of the Sultan Moulay Slimane University for the mineral analyses, using inductively coupled plasma optical emission spectroscopy (ICP-OES).
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This work was supported by the National Agency of Medicinal and Aromatic Plants (ANPMA-Morocco), National Center of Scientific Research (CNRST-Morocco) and Sultan Moulay Slimane University (USMS-Morocco), convention number 348/20.
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Lamsaadi, N., El Moukhtari, A., Oubenali, A. et al. Exogenous silicon improves salt tolerance of Fenugreek (Trigonella foenum-graecum L.) during seed germination and early seedling stages. Biologia 77, 2023–2036 (2022). https://doi.org/10.1007/s11756-022-01035-5
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DOI: https://doi.org/10.1007/s11756-022-01035-5