Abstract
Among all the cereals, wheat is an essential grain and food crop that provides a significant portion of the daily nutritional requirements for humans. To find the drought tolerant and drought sensitive genotypes, we employed fifty-three wheat genotypes, of which twenty-four genotypes were chosen based on physiological and biochemical criteria such as RWC, proline, and chlorophyll content. With the help of semi-quantitative RT-PCR and quantitative RT-PCR protocol, expression of dehydrin gene (WCOR410) and C-repeat binding factor genes such as CBF3, CBF4, CBF9, CBF5, CBF15, and 3H9 genes were studied in drought tolerant (C 306 and HD 2888) and sensitive genotypes (RAJ1555 and GW273) at the pre-anthesis stage. C306 and HD2888 genotypes identifies as a drought tolerant whereas RAJ1555 and GW273 genotypes shows less tolerance for drought stress condition. The majority of CBF genes were found to be up-regulated in some genotypes and down-regulated in others when exposed to water stress. In our study, we observed that genotype C306 had the highest amount of up-regulation when exposed to dehydration stress. These findings also show that the molecular response of plants to dehydration changes over time as a result of a combination of internal and external stressors. In the future, the identified tolerant variety could be used in wheat breeding for drought tolerant traits.
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Abbreviations
- ABA:
-
Abscisic acid
- ABRE:
-
ABA-responsive elements
- ANOVA:
-
Analysis of variance
- AP2:
-
Apetala2
- bHLH:
-
Basic helix–loop–helix
- bZIP:
-
Basic leucine zipper
- CBF:
-
C-repeat binding factor
- Ct:
-
Comparative threshold cycle
- DDF:
-
Dwarf and delayed flowering
- DREB:
-
Dehydration-responsive element binding
- EREBPs:
-
Ethylene-responsive element binding proteins
- MYB:
-
Myeloblastosis
- NLS:
-
Nuclear localization signal
- PCR:
-
Polymerase chain reaction
- RBD:
-
Random block design
- ROS:
-
Reactive oxidation species
- RWC:
-
Relative water content
- SPAD:
-
Soil plant analytical development
- SWC:
-
Soil water content
- TFs:
-
Transcription factors
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Acknowledgements
The authors thank Dr. Ramesh Singh, Associate Professor, Department of plant pathology, SVP University of agriculture & technology Meerut, India, for all the necessary help to carry out this research. Thanks to Director research SVPUAT for providing all the necessary facilities to conduct the research smoothly.
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KS and SPS did the field and wet experiments and data analysis. KS write the manuscript. MKY supervised the work.
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Singh, K., Singh, S.P. & Yadav, M.K. Physio-biochemical assessment and CBF genes expression analysis in wheat under dehydration condition. Biologia 77, 1851–1860 (2022). https://doi.org/10.1007/s11756-022-01028-4
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DOI: https://doi.org/10.1007/s11756-022-01028-4