Abstract
The sugars will eventually be exported transporters (SWEETs) are a unique class of membrane-bound sugar transporters that not only take part in hexose and sucrose transport across the biological membranes but also are involved in diverse physiological functions in plants. Although the role of SWEETs in biotic stress response is well documented, their involvement in plant’s response to abiotic and heavy metal (loid) stress is poorly understood. Thus, to bridge this gap, a comprehensive in silico study of the OsSWEET family was conducted in rice. A total of 21 OsSWEET members showing elaborate gene duplications were identified. Interestingly, the promoter cis-regulatory element and miRNA target analyses indicated the possible regulation of OsSWEETs during abiotic and heavy metal (loid) stresses. In-silico gene expression analysis further validated the involvement of OsSWEET transporters in abiotic and heavy metal (loid) stress responses in rice plants. Moreover, the protein-protein interaction analysis indicated their possible interactions with some well-known rice heavy metal transporter proteins. Taken together, this is the first comprehensive analysis of the involvement of OsSWEETs in abiotic, especially heavy metal (loid) stress responses in rice plants that necessitate further experimental studies to understand the mode of action and regulatory mechanisms of these transporters in heavy metal (loid) stress.
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MKM acknowledges Indian Institute of Technology Kharagpur for infrastructural support. SB acknowledges the research grant from Science and Engineering Research Board (SERB), Govt. of India (SRG/2019/001247) and Amity University Kolkata towards the development of infrastructural support.
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Experimental designs and analyses of results were carried out by SR, AS, BD, ND, MKM and SB. SB. The manuscript writing was carried out by SR, AS, BD, ND and SB. SB conceived the original research plan and together with MKM supervised the research work.
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Fig. S1
Homology modeling based tertiary structure models of OsSWEET transporters. (PNG 3330 kb)
Fig. S2
Prediction and distribution of all miRNA target sites on OsSWEET genes. The miRNAs which can target the OsSWEET genes were identified using the psRNATarget database, and the availability of the target sites of each miRNA on each gene is represented using different colours and patterns. (PNG 357 kb)
Fig. S3
Expression of OsSWEET genes under biotic stress. The expression of SWEET genes in rice under biotic stresses including Meloidogyne incognita, Magnaporthe orygae and Striga hermonthica was analyzed and represented using the fold change in expression. Green indicates the upregulation, while the downregulation is indicated using red colour. (PNG 244 kb)
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Roy, S., Sen, A., Das, B. et al. Genome-wide in silico analysis indicates the involvement of OsSWEET transporters in abiotic and heavy metal (loid) stress responses in rice. Biologia 77, 1737–1755 (2022). https://doi.org/10.1007/s11756-022-01022-w
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DOI: https://doi.org/10.1007/s11756-022-01022-w