Abstract
This study is to investigate the role and mechanism of Jianpi Jiedu decoction in psoriasis vulgaris. Seventy healthy male ICR mice were divided into control group, Acitretin capsule group, Jianpi decoction group, Jiedu decoction group, Jianpi Jiedu decoction group, spleen deficiency model group and psoriasis vulgaris with spleen deficiency group. The expression levels of IFN-γ, IL-6 and IL-17 in the drug intervention groups were significantly different from those in the control, the spleen deficiency model and the psoriasis vulgaris with spleen deficiency groups (P < 0.05). The psoriasis vulgaris with spleen deficiency group had obvious psoriasis vulgaris-like skin lesions, which were attenuated in the Jianpi Jiedu decoction group. The mRNA levels of STAT3 and STAT5 in Jianpi Jiedu decoction group were significantly lower than those in Jianpi decoction and Jiedu decoction groups (P < 0.05). At the protein level, STAT3 and STAT5 had no statistical significance among groups (P > 0.05). The protein expression levels of p-STAT3 and p-STAT5 in Jianpi Jiedu decoction group were significantly lower than those in Jianpi decoction and Jiedu decoction groups (P < 0.05). Conclusively, Jianpi Jiedu decoction may exert treatment effects on psoriasis vulgaris in mice with spleen deficiency via regulating JAKS/STATS pathway.
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Abbreviations
- JAK / STAT:
-
Janus Kinase / signal transducer and activator of transcription
- ICR:
-
Institute of Cancer Research
- IL-17:
-
Interleukin 17
- IL-6:
-
Interleukin 6
- IFN-γ:
-
Interferon-γ
- STAT3:
-
Signal transducer and activator of transcription 3
- STAT5:
-
Signal transducer and activator of transcription 5
- ELISA:
-
Enzyme-linked immunosorbent assay
- HE:
-
Hematoxylin-eosin
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Author contributions
Study Design: Chaoxia Liu, Hongxia Liu; Data Collection: Bin Li; Statistical Analysis: Shangping Yao; Data Interpretation: Liang Feng.
Funding
This work was supported by the Natural Science Foundation Project of Xinjiang Uygur Autonomous Region (2017D01C158) and State Administration of Traditional Chinese Medicine, 2016 National Famous Chinese Medicine Expert Hongxia Liu Heritage Studio Construction Project [Guo TCM Renjiaofa (2016) No. 42].
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All animal experiments were conducted according to the ethical guidelines of Xinjiang Uygur Traditional Chinese Medicine Hospital and were approved by the Ethics Committee of Xinjiang Uygur Traditional Chinese Medicine Hospital.
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Liu, C., Li, B., Yao, S. et al. The mechanism of Jianpi Jiedu decoction in treating psoriasis vulgaris in mice with spleen deficiency. Biologia 77, 1843–1850 (2022). https://doi.org/10.1007/s11756-021-00970-z
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DOI: https://doi.org/10.1007/s11756-021-00970-z