Abstract
PR-10 is an ubiquitous part of plant proteomes that are reported as homologues proteins to the Bet v 1, that was found previously to be a major birch-pollen allergen. In many plant species, PR-10 proteins are involved in allergic cross-reactions of sensitive patients. Despite their clinically relevance, PR-10 proteins are still not reported and characterized in every plant species yet. Here, a marker-based technique is reported to describe genomic variability of the length polymorphism based on the sequences of homologous genes to Bet v 1 group. BBAP (Bet v 1 homologs based amplified profile) method was applied to analyse the polymorphic variability of thirty fruit species and to analyse the generated fingerprint differences among them. A total number of generated loci was 682 and polymorphism reach 100%. The most different BBAP fingerprints were generated for Rubus nigra and the most similar BBAP profiles were obtained for Annona cherimola and Ananas comosus, Prunus dulcis and Malus domestica, and for Citrus sinensis and Citrus limon. The new type of DNA based molecular marker technique was aproved to be universal and an effective for mapping a variability of coding regions of plant genomes.
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Abbreviations
- BBAP:
-
Bet v 1 homologs Based Amplified Profile
- BLAST:
-
Basic Local Alignment Search Tool
- DNA:
-
Deoxyribonucleic Acid
- ITS:
-
Internal Transcribed Spacer
- NCBI:
-
National Centre of Bioinformatic Information
- PR proteins:
-
Pathogen-related proteins
- UPGMA:
-
Unweighted Pair Group Method with Arithmetic Mean
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This publication was supported by the Operational program Integrated Infrastructure within the project: Demand-driven research for the sustainable and innovative food, Drive4SIFood 313011 V336, cofinanced by the European Regional Development Fund (50%) and by the APVV SK-BY-RD-19-0014 grant: The formulation of novel compositions and properties study of the polysaccharides based edible films and coatings with antimicrobial and antioxidant plant additives (50%).
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JŽ and LU carried out all the analysis, data management and wrote this manuscript.
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Žiarovská, J., Urbanová, L. Utilization of Bet v 1 homologs based amplified profile (BBAP) variability in allergenic plants fingerprinting. Biologia 77, 517–523 (2022). https://doi.org/10.1007/s11756-021-00943-2
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DOI: https://doi.org/10.1007/s11756-021-00943-2