Abstract
Preservation of the brain is important to reducing recurrent seizures and other neurological sequelae after status epilepticus (SE). Medical ozone (MedO3) shows antioxidant, anti-inflammatory and anti-apoptotic properties in the human body. In this study, it was aimed to understand the neuroprotective potential of MedO3 in the acute period after SE. Mature rats of Wistar Albino were used for the study. Group design O3 + SE: SE induced after MedO3, O3: MedO3 alone was given, SE: SE only induced, SE + O3; MedO3 given after SE, C: control and S: sham control group. SE was induced with lithium-pilocarpine experimental model and evaluated on the Racine scale. Peripheral blood samples and brain tissue samples were taken before decapitation. Histopathological evaluation of the hippocampus of the rats given medical O3 before and after SE were studied. The highest peripheral blood oxidative stress index (OSI) was found in SE group. The OSI level in O3 + SE and SE + O3 was significantly higher than SE/C/S. Gene expressions of TNF-α and IL-1β mRNA were significantly higher in SE compared to other groups. Histopathologically; eosinophilia, cellular shrinkage and interstitial oedema were detected in the most severe SE and to a lesser extent in O3 + SE/ SE + O3. MedO3 reduced SE-induced oxidative damage, neuroinflammation and neuronal injury and contributed positively to neuroprotection.
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Abbreviations
- SE:
-
status epilepticus
- MedO3 :
-
medical ozone
- OSI:
-
oxidant stress index
- TNF-α:
-
tumour necrosis factor- alpha
- IL-1β:
-
interleukine-1beta
- Li-Pi:
-
lithium-pilocarpine
- (q)PCR:
-
(quantitative) real-timepolymerase chain reaction
- TAC:
-
total antioxidant capacity
- TOC:
-
total oxidant capacity
- H&E:
-
hematoxylin-eosin
- Group 1:
-
O3 + SE
- Group 2:
-
O3
- Group 3:
-
SE
- Group 4:
-
SE+ O3
- Group 5-control group:
-
C
- Group 6-sham control group:
-
(S)
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The financial funding of this study was supported by Çanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit (Project ID: 2697). All researchers thank the Scientific Research Projects Coordination Unit.
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Ethical approval was obtained for the study from Çanakkale Onsekiz Mart University Animal Experiments Local Ethics Committee for the study on 03.08.2018 with the file number 2018/1800109498. All the procedures performed in this study were carried in accordance with the 1964 Helsinki Declaration standards.
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Cokyaman, T., Oztopuz, O., Coskun, O. et al. The effect of medical ozone on oxidative stress and neuroinflammation in the early stage after experimental status epilepticus. Biologia 76, 3875–3882 (2021). https://doi.org/10.1007/s11756-021-00911-w
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DOI: https://doi.org/10.1007/s11756-021-00911-w