Abstract
Byrsonima verbascifolia Rich. Ex. A. Juss and Byrsonima intermedia (A) Juss are are important species due to the nutritional and pharmacological properties of their secondary metabolites. However, little is known about the action and attributes of these metabolites in the ecological interactions of these plants, as well as their possible cytogenotoxic effects. In this context, the objective of this study was to investigate the phytotoxicity of B. verbascifolia and B. intermedia leaf extracts in a bioassay with Lactuca sativa L. Aqueous extracts (cold and hot) and ethanolic leaf extract were obtained at a concentration of 5% (w/v) and, subsequently, solutions at concentrations 20, 40, 60, 80 and 100% were prepared. The following were evaluated: germination percentage, germination speed index, number of normal seedlings, root elongation, shoot length, fresh and dry biomass, mitotic index and chromosomal abnormalities. Data were submitted to analysis of variance and the means were compared using the Scott-Knott test at 5% significance. The results show that B. verbascifolia and B. intermedia leaf extracts have phytotoxic and cytotoxic potential. These effects differ as a function of the form of extraction in which the ethanolic extract showed greater toxicity regardless of the species. For most of the analyzed parameters, there was no statistical difference between species but, for number of normal seedlings, B. verbascifolia was more toxic and, for root elongation, B. intermedia presented greater toxicity. Among chromosomal abnormalities, C-metaphase and stickiness were the most frequent, indicating an aneugenic effect of Byrsonima spp. leaf extracts.
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References
Alves RJR, da Silva MAD, Alves RM, Dvoskin DM, de Moura DP, da Silva LM (2020) Influência alelopática do extrato aquoso de folíolos frescos de Mimosa tenuiflora (Willd.) Poir. sobre a germinação de Bidens pilosa L. Res Soc Dev 9(9). https://doi.org/10.33448/rsd-v9i9.8061
Amâncio BCS, Govêa KP, Trindade LDOR, Cunha Neto AR, de Souza TC, Barbosa S (2020) Sandwich method applied to the screening of allelopathic action in Byrsonima spp (Malpighiaceae). Biol Brat 75(2):175–182. https://doi.org/10.2478/s11756-019-00369-x
Araújo ECG, Silva TC, Lima TV (2018) Efeitos alelopáticos de Sesbania virgata (cav.) Pers na germinaçao de sementes de alface. Rev Eng Agric 26(02):101–109. https://doi.org/10.13083/reveng.v26i2.862
Araújo SS, Fernandes TCC, Cardona YT, de Almeida PM, Marin-Morales MA, Dos Santos AV, Brasileiro-Vidal AC (2015) Cytotoxic and genotoxic effects of ethanolic extract of Euphorbia hyssopifolia L. on HepG2 cells. J Ethnopharmacol (170):16–19. https://doi.org/10.1016/j.jep.2015.04.044
Belwal T, Ezzat SM, Rastrelli L, Bhatt ID, Daglia M, Baldi A, Anandharamakrishnan C (2018) A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies. TrAC Trends Analyt Chem 100:82–102. https://doi.org/10.1016/j.trac.2017.12.018
Borges LP, Amorim VA (2020) Metabólitos secundários de plantas secondary plant metabolites. Rev Agrotecnol 11: 54–67. https://core.ac.uk/display/288224916?utm_source=pdf&utm_medium=banner&utm_campaign=pdf-decoration-v1. Accessed 19 June 2021
Castro AHF, Braga KDQ, Sousa FMD, Coimbra MC, Chagas RCR (2016) Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC.(Malpighiaceae). Rev Ciênc Agron 47:143–151. https://doi.org/10.5935/1806-6690.20160017
Chiapusio G, Sanchez AM, Reigosa MJ, Gonzalez L, Pellissier F (1997) Do germination indices adequately reflect allelochemical effects on the germination process? J Chem Ecol 23(11):2445–2453. https://doi.org/10.1023/B:JOEC.0000006658.27633.15
Ferreira DF (2014) Sisvar: a guide for its bootstrap procedures in multiple comparisons. Ciênc Agrotec 38(2):109–112. https://doi.org/10.1590/S1413-70542014000200001
Ferreira MC, Souza JRP, Faria TJ (2007) Potencial alelopático de extratos vegetais na germinação e no crescimento inicial de picão-preto e alface. Ciênc Agrotec 31:1054–1060. https://doi.org/10.1590/S1413-70542007000400017
Fraige K, Dametto AC, Zeraik ML, de Freitas L, Saraiva AC, Medeiros AI, Castro-Gamboa I, Cavalheiro AJ, Silva DHS, Lopes NP, Bolzani VS (2018) Dereplication by HPLC-DAD‐ESI‐MS/MS and Screening for Biological Activities of Byrsonima Species (Malpighiaceae). Phytochem Anal 29(2):196–204. https://doi.org/10.1002/pca.2734
Freitas JV, Batitucci MDCP, Andrade MA, da Luz AC, Pereira UJ (2015) Prospecção fitoquímica e avaliação da citotoxicidade e genotoxicidade de Helenium cf. amarum (Raf.) H. Rock. Rev Cub de Plantas Medicinales 19(4). Available in: http://www.revplantasmedicinales.sld.cu/index.php/pla/article/view/83/92. Accessed 18 June 2019
Giotto AC, Oliveira SCC, Joelma GPS (2007) Efeito alelopático de Eugenia dysenterica Mart. ex DC. Berg. (Myrtaceae) na germinação e no crescimento de Lactuca sativa L. (Asteraceae). Rev Bras Biociênc 5:600–602. http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/526/449. Accessed 18 June 2019
Haq RA, Hussain M, Cheema ZA, Mushtaq MN, Farooq M (2010) Mulberry leaf water extract inhibits bermudagrass and promotes wheat growth. Weed Biol Manag 10:234–240. https://doi.org/10.1111/j.1445-6664.2010.00389.x
Leme DM, Marrin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its applications. Mutat Res 682:71–78. https://doi.org/10.1016/j.mrrev.2009.06.002
Maguire JD (1962) Speeds of germination-aid selection and evaluation for seedling emergence and vigor. Crop Sci 2:176–177. https://www.crops.org/publications/cs/abstracts/2/2/CS0020020176. Accessed 18 June 2021
Moraes RM, dos Santos Filho PR, Carvalho M, de Lima Nogueira M, Barbosa S (2015) Effects of copper on physiological and cytological aspects in Lactuca sativa L. Rev Bras Biocienc 13(2). http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/3307. Accessed 18 Jun 2019
Neto MVL, Malheiros RSP, Santana FS, Machado LL, Mapeli AM (2014) Avaliação alelopática de extratos etanólicos de Copaifera sabulicola sobre o desenvolvimento inicial de Lactuca sativa, Lycopersicum esculentum e Zea mays. Biotemas 27(3):23–32. https://doi.org/10.5007/2175-7925.2014v27n3p23
Odeigah PGC, Nurudeen O, Amund OO (1997) Genotoxicity of oil field wastewater in Nigeria. Hereditas 126:161–167. https://doi.org/10.1111/j.1601-5223.1997.00161.x
Paula C, Canteli V, Silva C, Miguel O, Miguel M (2015) Study of phytotoxic potential of extracts of Bauhinia ungulata L. on cell division and enzyme activity in lettuce seedlings. Rev Bras Plantas Med 17(4):577–584. https://doi.org/10.1590/1983-084X/13_079
Pinheiro PF, Costa AV, Alves TDA, Galter IN, Pinheiro CA, Pereira AF, Fontes MMP (2015) Phytotoxicity and cytotoxicity of essential oil from Leaves of Plectranthus amboinicus, carvacrol, and thymol in plant bioassays. J Agric Food Chem 63(41):8981–8990. https://doi.org/10.1021/acs.jafc.5b03049
Pinto GFS, Kolb RM (2015) Seasonality affects phytotoxic potential of five native species of Neotropical savanna. Botany 94(999):1–9. https://doi.org/10.1139/cjb-2015-0124
Ramos LTT, de Araújo Rodrigues P, Soares RA, Guedes MIF (2020) Avaliação da atividade antinflamatória do extrato etanólico de Byrsonima sericea em edema de pata induzido por carragenina em camundongos. Braz J of Dev 6(7):48734–48742. https://doi.org/10.34117/bjdv6n7-499
Ribeiro LO, Barbosa S, Balieiro FP, Beijo LA, Santos BR, Gouvea CMCP, Paiva LV (2012) Fitotoxicidade de extratos foliares de barbatimão [Stryphnodendron adstringens (Mart.) Coville] em bioensaio com alface. Rev Bras Biociênc 10(2):220–225. http://www.ufrgs.br/seerbio/ojs/index.php/rbb/article/view/2139. Accessed 18 June 2019
Ribeiro LR, Santos MF, Silva QM, Palmieri MJ, Andrade-Vieira L, Davide LC (2013) Cytogenotoxic effects of ethanolic extracts of Annona crassiflora (Annonaceae). Biologia 68(3):433–438. https://doi.org/10.2478/s11756-013-0185-3
Saldanha AA, Carmos LF, Nascimento SB, Matos NA, Veloso CC, Castro AHF, De Vos RCH, Klein A, Siqueira JM, Carollo CA, Nascimento TV, Toffoli-Kadri MC, Soares AC (2016) Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia. J Nat Med 70:760–768. https://doi.org/10.1007/s11418-016-1011-3
Silva SF, Costa HSL, Viana JS, Medeiros Filho S (2018) Phytotoxicity of exotic species on the physiological potential of crambe seeds (Crambe abyssinica Hochs). Rev Agroambiente 12:89–95. https://doi.org/10.18227/1982-8470ragro.v12i1.4329
Simões MS, Mandail RH, Barbosa S, Nogueira ML (2013) Padronizaçãoo de bioensaios para detecção de compostos alelopaticos e toxicantes ambientais utilizando alface. Biotemas 26(3):29–36. https://doi.org/10.5007/2175-7925.2013v26n3p29
Souza Filho APS, Guilhon GMSP, Santos LS (2010) Metodologias empregadas em estudos de avaliação da atividade alelopática em condições de laboratório-Revisão crítica. Planta Daninha 28(3):689–697. https://doi.org/10.1590/S0100-83582010000300026
Acknowledgements
The authors would like to thank Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES) for the postdoctoral fellowship PNPD to Dra. Luciene de Oliveira Ribeiro Trindade and fellowship PET to Professor Sandro Barbosa, National Council for Scientific and Technological Development (CNPq) and Foundation for Research Support of the State of Minas Gerais (FAPEMIG) for the financial support.
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Amâncio, B.C.S., Govêa, K.P., Neto, A.R.C. et al. Allelopathic activity of different Byrsonima spp. leaf extracts on Lactuca sativa L. bioassay. Biologia 76, 3201–3209 (2021). https://doi.org/10.1007/s11756-021-00872-0
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DOI: https://doi.org/10.1007/s11756-021-00872-0