Abstract
South Atlantic hydrographic basin comprises several small coastal drainages isolated from each other and the basins of the Brazilian crystalline shield. Tramandaí River and Patos Lagoon sub-basins occur at the southern end of this system characterized by a high level of ichthyofaunistic endemism. In the present study, we investigated the karyotype of six species of whiptails armoured catfishes from these two hydrographic basins. The results revealed interspecific diploid number variations: 2n = 64 in Rineloricaria malabarbai and R. cadeae, 2n = 68 in R. microlepdogaster, and R. aequalicuspis and 2n = 70 in R. quadrensis and R. longicauda. Species with te same diploid number are easily discriminated by karyotype formulas and the number of 5S rDNA sites. Our study provides a set of efficient chromosome markers to confirm the taxonomic status of six Rineloricaria species from Patos Lagoon and Tramandai River basins. We believe that successive vicariant process promote geographical isolation enhancing the fixation of chromosomal rearrangements, leading to the observed karyotypic variabilities. This is a plausible hypothesis if we consider that the current configuration of Rio Grande do Sul coastal drainages was shaped by a complex interation of geomofological processes and Pleistocenic/Holocenic sea-level fluctuations.
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Data availability
All specimens analyzed in this study was deposited on the Ichthyological colletion of Museu de Zoologia da Universidade de Londrina (MZUEL). The vouchers numbers are available online in specieslink platform.
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Acknowledgments
The authors are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES - Finance code 001) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for their financial support; at Dr. Luiz R. Malabarba, who provided all the necessary structure for the collection and maintenance of the specimens, and Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) for permitting the collection of biological material.
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NBV Designed the study, performed the experiments, and drafted the manuscript. FHT and LPS performed some FISH experiments and drafted the manuscript. MSR identified the species and revised the manuscript. LGC and RR revised the manuscript. All authors read and approved the final version of the manuscript.
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Venturelli, N.B., Takagui, F.H., Pompeo, L.R.S. et al. Cytogenetic markers to understand chromosome diversification and conflicting taxonomic issues in Rineloricaria (Loricariidae: Loricariinae) from Rio Grande do Sul coastal drainages. Biologia 76, 2561–2572 (2021). https://doi.org/10.1007/s11756-021-00748-3
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DOI: https://doi.org/10.1007/s11756-021-00748-3