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Effect of bivalirudin on neointimal hyperplasia and endothelial proliferation in rabbit

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Abstract

Background

The aim of this study was to investigate the effects of bivalirudin on endothelial cell proliferation and neointimal hyperplasia in a rabbit carotid artery model.

Methods

“New Zealand rabbits (n = 12)” weighing 2–3 kg were randomly divided into two groups. Arteriotomy was performed to the rabbit carotid artery and closed with continuous suture technique. Group B (n = 6) as a control group received 150 U/kg heparin sodium; however, group A (n = 6) was given 0.75 mg/kg bivalirudin i.v. bolus and infusion 1.75 mg/kg/hour (B01AE06-Bivalirudin 250 mg) during perioperation period. At the end of the 28th day, the carotid artery segment was excised and evaluated histologically.

Results

All histological and immune staining analyzes were performed by two blind researchers in the treatment of rabbits. In the control group rabbit carotid artery sections, tunica intima was observed to thicken. In the bivalirudin group, intimal hyperplasia was less observed compared to the control group. No significant difference was observed between groups in tunica media thickness. Lumen diameter and lumen area were found to be wider in the experimental group. P value was found to be less than 0.05.

Conclusion

Our study demonstrates that bivalirudin significantly affects and prevents neointimal hyperplasia and endothelial cell proliferation.

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Funding

The authors received no financial support for the research or authorship of this article.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Faculty of Medicine, Dokuz Eylul University, İzmir, Turkey

    Tugra Gencpinar & Serdar Bayrak

  2. Department of Cardiovascular Surgery, Behcet Uz Children’s Training and Research Hospital, İzmir, Turkey

    Cagatay Bilen

  3. Medical Laboratory Technigues Programme, Izmir Kavram Vocational School, İzmir, Turkey

    Pinar Akokay

  4. Department of Radiology, Faculty of Medicine, Dokuz Eylul University, İzmir, Turkey

    Mustafa Baris

  5. Department of Cardiovascular Surgery, Bozyaka Training and Research Hospital, İzmir, Turkey

    Baris Kemahli

Authors
  1. Tugra Gencpinar
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  2. Serdar Bayrak
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  3. Cagatay Bilen
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  4. Baris Kemahli
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  5. Pinar Akokay
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  6. Mustafa Baris
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  7. Cenk Erdal
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Contributions

Concept—PA and TG; design—TG and BK; supervision—SB, BK, PA, and MB; resource—CB; materials—TG; data collection and/or processing—TG and CE; analysis—CE and CB; literature search—TG, MB, and CB; writing—TG and BK; critical review—CB; other—PA. All authors approved the final version of the manuscript.

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Correspondence to Tugra Gencpinar.

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Gencpinar, T., Bayrak, S., Bilen, C. et al. Effect of bivalirudin on neointimal hyperplasia and endothelial proliferation in rabbit. Gen Thorac Cardiovasc Surg 69, 425–433 (2021). https://doi.org/10.1007/s11748-020-01454-8

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  • DOI: https://doi.org/10.1007/s11748-020-01454-8

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