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Impact of chronic cyanosis and reoxygenation on the microheterogeneity of the myocardial blood flow: digital radiographic study in neonatal rats

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Abstract

Purpose

This study sought to show the heterogeneity of myocardial blood flow in the chronically hypoxic infantile myocardium and its response to reoxygenation using a novel type of digital radiography.

Methods

Newborn rats were housed in a hypoxic chamber or in a normal chamber (controls). After 4 or 8 weeks, the control rats were ventilated with normoxic conditions, and the rats housed under hypoxia were ventilated with either hypoxic (cyanotic group) or normoxic conditions (reoxygenation group). Desmethylimipramine labeled with tritium (HDMI) was injected into the left ventricle, and both ventricular free walls were sectioned and sliced from the subepicardium to the subendocardium at 10 mm thickness. The within-layer distribution of HDMI density was measured by digital radiography, and its spatial heterogeneity (i.e., flow heterogeneity) was quantified by the coefficient of variation (CV) of flows.

Results

There were no differences in the CV between the groups in either ventricle at 4 weeks of age and no differences in the right ventricle at 8 weeks of age. There was a trend toward a higher left ventricular CV in the cyanotic group than in the control group at 8 weeks of age (0.637 ± 0.099 vs. 0.510 ± 0.060, P = 0.06). At 8 weeks of age, the CV was lower in both ventricles in the reoxygenation group than in those of the control and cyanotic groups.

Conclusion

The chronically hypoxic infantile myocardium exhibits regional flow heterogeneity similar to that observed in the normal myocardium in both ventricles and exhibits reduced flow heterogeneity in response to reoxygenation.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan

    Tomoko Tomii, Yasuhiro Fujii & Shunji Sano

  2. Division of Cardiovascular Surgery, The Hospital for Sick Children, The University of Toronto, 555 University Avenue, Toronto, ON, M5G 1X8, Canada

    Osami Honjo

  3. The Labatt Family Heart Centre, The Hospital for Sick Children, The University of Toronto, Toronto, Canada

    Osami Honjo

  4. Department of Mechanical Science and Bioengineering, Osaka University Graduate School of Engineering Science, Toyonaka, Japan

    Takeshi Matsumoto

  5. Department of Medical Engineering, Faculty of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan

    Hiroyuki Tachibana

  6. Circulatory Center, Showa University Northern Yokohama Hospital, Yokohama, Japan

    Kozo Ishino

  7. Department of Medical Engineering and Systems Cardiology, Kawasaki Medical School, Kurashiki, Japan

    Yasuo Ogasawara

Authors
  1. Tomoko Tomii
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  2. Osami Honjo
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  3. Takeshi Matsumoto
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  4. Hiroyuki Tachibana
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  5. Yasuhiro Fujii
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  6. Kozo Ishino
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  7. Yasuo Ogasawara
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  8. Shunji Sano
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Corresponding author

Correspondence to Osami Honjo.

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Tomii, T., Honjo, O., Matsumoto, T. et al. Impact of chronic cyanosis and reoxygenation on the microheterogeneity of the myocardial blood flow: digital radiographic study in neonatal rats. Gen Thorac Cardiovasc Surg 59, 672–680 (2011). https://doi.org/10.1007/s11748-010-0685-9

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  • DOI: https://doi.org/10.1007/s11748-010-0685-9

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