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Stability of vitamin A in oil-in-water-in-oil-type multiple emulsions

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Journal of the American Oil Chemists' Society

Abstract

The stabilityof vitamin A was studied in thee different emulsions: oil-in-water (O/W), water-in-oil (W/O), and oil-in-water-in-oil (O/W/O). The stability of retinol (vitamin A alcohol) in the O/W/O emulsion was the highest among the thee types of emulsions; remaining percentages at 50°C after 4 wk in the O/W/O, W/O, and O/W emulsions were 56.9, 45.7, and 32.3, respectively. With increasing peroxide value of O/W and W/O emulsifiers, the remaining percentage of vitamin A palmitate and retinol in the emulsions decreased significantly, indicating that peroxides in the formulae accelerate the decomposition of vitamin A. Organophilic clay mineral (an oil gelling agent and a W/O emulsifier) also affected the stability of retinol; synthesized saponite was better than naturally occurring bentonite for retinol stability. The stability of retinol in the O/W/O emulsion increased with increasing inner oil phase ratio (φi), whereas in O/W it was unaffected by φi. Encapsulation percent of retinol in the O/W/O emulsion, the ratio of retinol in the inner oil phase to the total amount in the emulsion, increased with increasing φi. The remaining percent of retinol in the O/W/O emulsion was in excellent agreement with encapsulation percent, suggesting that retinol in the inner oil phase is more stable than that in the outer oil phase. Addition of antioxidants (tert-butylhydroxytoluene, sodium ascorbate, and EDTA) to the O/W/O emulsion improved the stability of retinol up to 77.1% at 50°C after 4 wk. We conclude that the O/W/O emulsion is a useful formula to stabilize vitamin A.

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Authors and Affiliations

  1. Shiseido Basic Research Laboratories, 1050 Nippa Kohoku, 223-8553, Yokohama, Japan

    Katsunori Yoshida, Tomoko Sekine, Fumiaki Matsuzaki, Toshio Yanaki & Michihiro Yamaguchi

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  1. Katsunori Yoshida
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  2. Tomoko Sekine
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  3. Fumiaki Matsuzaki
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  4. Toshio Yanaki
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  5. Michihiro Yamaguchi
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Correspondence to Toshio Yanaki.

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Yoshida, K., Sekine, T., Matsuzaki, F. et al. Stability of vitamin A in oil-in-water-in-oil-type multiple emulsions. J Amer Oil Chem Soc 76, 1–6 (1999). https://doi.org/10.1007/s11746-999-0212-2

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  • DOI: https://doi.org/10.1007/s11746-999-0212-2

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