Abstract
Resolution of fatty acid methyl esters (FAME) by thin-layer chromatography often is complicated by co-migration of certain acyl-isomers in heterogeneous mixtures. However, a novel reversed-phase thin-layer chromatography method which employs 10% (wt/vol) silver nitrate in a mobile phase containing acetonitrile/1,4-dioxane/acetic acid (80:20:1, vol/vol/vol) allows one-dimensional resolution of a wide range of acyl-methyl esters. This innovation enables improved separation of saturated FAME ranging from C12 to C22, and geometric isomers of C14 to C22 unsaturated FAME by thin-layer chromatography.
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Marquardt, T.C., Wilson, R.F. An improved reversed-phase thin-layer chromatography method for separation of fatty acid methyl esters. J Amer Oil Chem Soc 75, 1889–1892 (1998). https://doi.org/10.1007/s11746-998-0346-7
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DOI: https://doi.org/10.1007/s11746-998-0346-7