Abstract
A mixture of unsaturated fatty acids (FA), mainly linolenic acid, and bovine serum albumin (BSA) was incubated under several different atmospheres to study the effect of these atmospheres on the stability of FA to oxidation. Four experiments were carried out simultaneously, which consisted of the incubation of the FA/BSA mixture under air, nitrogen, air in the presence of 200 ppm of butylated hydroxytoluene, and air for 6 h and then under nitrogen. The four experiments were tested for lipid oxidation and color changes by measuring absorbances at 234 and 270 nm, formation of thiobarbituric acid-reactive substances, and color differences and yellowness index. The samples that were oxidized with air before storage under nitrogen were the most stable against lipid peroxidation and exhibited the smallest color changes. These results suggest that a slight and controlled lipid oxidation improved the oxidative stability of FA/BSA mixtures.
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References
Lipid Oxidation in Food, edited by A.J. St. Angelo, American Chemical Society, Washington, D.C., 1992.
Berger, K.G., and R.J. Hamilton, Lipids and Oxygen: Is Rancidity Avoidable in Practice? in Developments in Oils and Fats, edited by R.J. Hamilton, Blackie, London, 1995, pp. 192–203.
Smouse, T.H., Significance of Lipid Oxidation to Food Processor, in Food Lipids and Health, edited by R.E. McDonald and D.B. Min, Marcel Dekker, New York, 1996, pp. 269–286.
Hamilton, R.J., The Chemistry of Rancidity in Foods, in Rancidity in Foods, 3rd edn., edited by J.C. Allen and R.J. Hamilton, Blackie, London, 1994, pp. 1–21.
Smouse, T.H., Factors Affecting Oil Quality and Stability, in Methods to Assess Quality and Stability of Oils and Fat-Containing Foods, edited by K. Warner and N.A.M. Eskin, AOCS Press, Champaign, 1994, pp. 17–36.
Jadhav, S.J., S.S. Nimbalkar, A.D. Kulkarni, and D.L. Madhavi, Lipid Oxidation in Biological and Food Systems, in Food Antioxidants. Technological, Toxicological and Health Perspectives, edited by D.L. Madhavi, S.S. Deshpande, and D.K. Salunkhe, Marcel Dekker, New York, 1996, pp. 5–63.
Gunstone, F.D., Chemical Properties, in The Lipid Handbook, edited by F.D. Gunstone, J.L. Harwood, and F.B. Padley, Chapman and Hall, London, 1986, pp. 449–484.
Nawar, W.W., Lipids, in Food Chemistry, 3rd edn., edited by O.R. Fennema, Marcel Dekker, New York, 1996, pp. 225–319.
Ucciani, E., and A. Debal, Chemical Properties of Fats, in Oils and Fats Manual. A Comprehensive Treatise, edited by A. Karleskind, Lavoisier Publishing, Paris, 1996, pp. 325–443.
Zamora, R., and F.J. Hidalgo, Antioxidant Activity of the Compounds Produced in the Nonenzymatic Browning Reaction of (E)-4,5-Epoxy-(E)-2-Heptenal and Lysine, in Progress in Food Fermentation, edited by C. Benedito de Barber, C. Collar, M.A. Martínez-Anaya, and J. Morell, IATA, CSIC, Valencia, Spain, 1993, Vol. 2, pp. 540–545.
Alaiz, M., R. Zamora, and F.J. Hidalgo, Natural Antioxidants Produced in Oxidized Lipid/Amino Acid Browning Reactions, J. Am. Oil Chem. Soc. 72:1571–1575 (1995).
Alaiz, M., F.J. Hidalgo, and R. Zamora, Antioxidative Activity of Nonenzymatically Browned Proteins Produced in Oxidized Lipid/Protein Reactions, J. Agric. Food Chem. 45:1365–1369 (1997).
Alaiz, M., R. Zamora, and F.J. Hidalgo, Contribution of the Formation of Oxidized Lipid/Amino Acid Reaction Products to the Protective Role of Amino Acids in Oils and Fats, Ibid.:1890–1895 (1996).
Zamora, R., M. Alaiz, and F.J. Hidalgo, Feed-Back Inhibition of Oxidative Stress by Oxidized Lipid/Amino Acid Reaction Products, Biochemistry 36:15765–15771 (1997).
Zamora, R., and F.J. Hidalgo, Linoleic Acid Oxidation in the Presence of Amino Compounds Produces Pyrroles by Carbonyl Amine Reactions, Biochim. Biophys. Acta 1258:319–327 (1995).
Hunter, R.S., The Measurement of Appearance, Hunter Associates Laboratory, Fairfax, VA, 1973.
Commission Internationale de l’Eclairage, Recommendations on Uniform Color Spaces, Color-Differences Equations, Psychometric Color Terms, CIE Publication 15 (Supplement 2), Bureau Central de la Commission Internationale de l’Eclairage, Paris, 1978.
Francis, F.J., and F.H. Clydesdale, Food Colorimetry: Theory and Applications, AVI Publishing, Westport, CT, 1975.
Kosugi, H., T. Kojima, and K. Kikugawa, Thiobarbituric Acid-Reactive Substances from Peroxidized Lipids, Lipids 24:873–881 (1989).
Snedecor, G.W., and W.G. Cochran, Statistical Methods, 7th edn., Iowa State University Press, Ames, 1980.
Frankel, E.N., M.L. Hu, and A.L. Tappel, Rapid Headspace Gas Chromatography of Hexanal as a Measure of Lipid Peroxidation in Biological Samples, Lipids 24:976–981 (1989).
Venolia, A.W., and A.L. Tappel, Brown-Colored Oxypolymers of Unsaturated Fats, J. Am. Oil Chem. Soc. 35:135–138 (1958).
Tappel, A.L., The Mechanism of Vitamin E Action. III. In Vitro Copolymerization of Oxidized Fats with Protein, Arch. Biochem. Biophys. 54:266–280 (1955).
Hidalgo, F.J., and R. Zamora, Non-Enzymatic Browning and Fluorescence Development in a (E)-4,5-Epoxy-(E)-2-heptenal/Lysine Model System, J. Food Sci. 58:667–670 (1993).
Desai, I.D., and A.L. Tappel, Damage to Proteins by Peroxidized Lipids, J. Lipid Res. 4:204–207 (1963).
Gardner, H.W., Lipid Hydroperoxide Reactivity with Proteins and Amino Acids: A Review, J. Agric. Food Chem. 27:220–229 (1979).
Karel, M., K. Schaich, and R.B. Roy, Interaction of Peroxidizing Methyl Linoleate with Some Proteins and Amino Acids, Ibid.:159–163 (1975).
Alaiz, M., R. Zamora, and F.J. Hidalgo, Antioxidative Activity of Pyrrole, Imidazole, Dihydropyridine, and Pyridinium Salt Derivatives Produced in Oxidized Lipid/Amino Acid Browning Reactions, Ibid.:686–691 (1996).
Ahmad, I., M. Alaiz, R. Zamora, and F.J. Hidalgo, Antioxidative Activity of Lysine/13-Hydroperoxy-9(Z),11(E)-octadecadienoic Acid Reaction Products, Ibid.:3946–3949 (1996).
Hidalgo, F.J., and R. Zamora, Fluorescent Pyrrole Products from Carbonyl-Amine Reactions, J. Biol. Chem. 268:16190–16197 (1993).
Hidalgo, F.J., and R. Zamora, In vitro Production of Long Chain Pyrrole Fatty Esters from Carbonyl-Amine Reactions, J. Lipid Res. 36:725–735 (1995).
Zamora, R., J.L. Navarro, and F.J. Hidalgo, Determination of Lysine Modification Product ɛ-N-Pyrrolylnorleucine in Hydrolyzed Proteins and Trout Muscle Microsomes by Micellar Electrokinetic Capillary Chromatography, Lipids 30:477–483 (1995).
Alaiz, M., R. Zamora, and F.J. Hidalgo, Antioxidative Activity of (E)-2-Octenal/Amino Acids Reaction Products, J. Agric. Food Chem. 43:795–800 (1995).
Frankel, E.N., In Search of Better Methods to Evaluate Natural Antioxidants and Oxidative Stability in Food Lipids, Trends Food Sci. Technol. 4:220–225 (1993).
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Alaiz, M., Hidalgo, F.J. & Zamora, R. Effect of initial slight oxidation on stability of polyunsaturated fatty acid/protein mixtures under controlled atmospheres. J Amer Oil Chem Soc 75, 1127–1133 (1998). https://doi.org/10.1007/s11746-998-0124-6
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DOI: https://doi.org/10.1007/s11746-998-0124-6