Journal of the American Oil Chemists' Society

, Volume 75, Issue 1, pp 15–19 | Cite as

Rapid determination of iodine value by 1H nuclear magnetic resonance spectroscopy

  • Yuko Miyake
  • Kazuhisa Yokomizo
  • Narihide Matsuzaki


High-resolution 1H nuclear magnetic resonance (1H NMR) has been found to be an effective tool for the direct, rapid, and automated determination of the iodine value (IV) of vegetable oils, including hydrogenated oils (IV=45.9–140.2). The total time required to obtain the 1H NMR data is about 3 min per sample. The IV is calculated from the number of double-bonded protons and the average molecular weight derived directly from the spectrum. The average of olefinic protons and allylic plus divinyl protons area was used to calculate the absolute number of double-bonded protons. The 1H NMR results were compared with those obtained by the traditional Wijs-cyclohexane methods. The correlation coefficient between traditional IV and the novel 1H NMR method was r2=0.9994 for the regression equation Y=0.9885X + 2.8084, where X was the result given by the traditional method. With the proposed regression equation, IV calculated by the 1H NMR method was within an error of ± 1 unit of the result obtained by the traditional method. The proposed method is practically viable if one can afford to have the NMR system.

Key words

1H NMR spectroscopy hydrogenated oil iodine value vegetable oil 


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  1. 1.
    Sato, T., S. Kawano, and M. Iwamoto, Near Infrared Spectra Patterns of Fatty Acid Analysis from Fats and Oils, J. Am. Oil Chem. Soc. 68:827–833 (1991).Google Scholar
  2. 2.
    Pfeffer, P.E., F.E. Luddy, and J. Unruh, Analytical 13CNMR: A Rapid, Nondestuctive Method for Determining the cis,trans Composition of Catalytically Treated Unsaturated Lipid Mixture, Ibid.380–386 (1977).Google Scholar
  3. 3.
    Saito, H., Estimation of the Oxidative Determination of Fish Oils by Measurement of Nuclear Magnetic Resonance, Agric. Biol. Chem. 51:3433–3435 (1987).Google Scholar
  4. 4.
    Sacchi, R., I. Medina, S.P. Aubourg, F. Addeo, and L. Paolillo, Proton Nuclear Magnetic Resonance Rapid and Structure-Specific Determination of ω-3 Polyunsaturated Fatty Acids in Fish Lipids, J. Am. Oil Chem. Soc. 70:225–228 (1993).CrossRefGoogle Scholar
  5. 5.
    Aursand, M., H. Jorgensen, and H. Grasdalen, Positional Distribution of ω-3 Fatty Acids in Marine Lipids Triacylglycerols by High-Resolution 13C Nuclear Magnetic Resonance Spectroscopy, Ibid293–297 (1995).CrossRefGoogle Scholar
  6. 6.
    Diehlund, B.W.K., and W. Ockels, Erimittlunder Fettsaureverteilung durch 13C-NMR Spectroskopie, Fat Sci. Technoi. 97:115–118 (1995).Google Scholar
  7. 7.
    Brosio, E., F. Conti, A. Dinola, and S. Sykora, Correlation Between Iodine Number and Proton Relaxation Times in Maize Oil, J. Fd. Technol. 16:67–72 (1981).Google Scholar
  8. 8.
    Matsui, T., H. Iwasaki, K. Matsumoto, and Y. Osajima, Quality Evaluation of Edible Oils by Proton Nuclear Magnetic Resonance Measurement, Food Sci. Technol. Int. 1:94–97 (1995).Google Scholar
  9. 9.
    Sarpal, A.S., G.S. Kapur, S. Mukherjee, K.S. Jayparkash, and S.K. Jain, Determination of Iodine Value of Lubricating Oils by Nuclear Magnetic Resonance (NMR) Spectroscopy, Lubricasion Eng. 51:209–214 (1995).Google Scholar
  10. 10.
    Watanabe, S., Y. Murase, Y. Inokuchi, T. Okamoto, S. Kumita, T. Shikama, Y. Tanaka, and Y. Hasegawa, Determination of Iodine Value Using Alternative Solvent to Carbon Tetrachloride—Results of Collaborative Study, J. Jpn. Oil Chem. Soc. (Yukagaku) 40:592–600 (1991).Google Scholar
  11. 11.
    Wanasundara, U.N., and F. Shahidi, Application of NMR Specroscopy to Assess Oxidative Stability of Canola and Soybean Oils, J. Fd. Lipids 1:15–24 (1993).CrossRefGoogle Scholar

Copyright information

© AOCS Press 1988

Authors and Affiliations

  • Yuko Miyake
    • 1
  • Kazuhisa Yokomizo
    • 1
  • Narihide Matsuzaki
    • 1
  1. 1.Oils & Vegetable Proteins Laboratories, Food Research & Development LaboratoriesAjinomoto Co., Inc.Yokohama-shiJapan

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