Journal of the American Oil Chemists' Society

, Volume 74, Issue 3, pp 317–321 | Cite as

Regular-sized cell creation in microchannel emulsification by visual microprocessing method

  • Takahiro Kawakatsu
  • Yuji Kikuchi
  • Mitsutoshi Nakajima


A novel emulsification method was developed for making monodispersed regular-sized cells. Both oil in water (O/W) and water in oil (W/O) emulsion cells were generated by permeating an internal phase into a continuous phase through a silicon microchannel, which was designed and prepared by using semiconductor technology. The microprocessing of O/W (or W/O) emulsion cells was monitored and controlled with a microscope video system. Regular-sized O/W cells were made by a normal hydrophilic microchannel and a glass plate with use of an appropriate surfactant. On the other hand, W/O emulsion cells were made by a hydrophobic microchannel and a glass plate modified with a silane coupler reagent. Regular-sized W/O cells were also obtained; therefore, a suitable combination of organic phase, surfactant, and electrolyte should be carefully selected. There is a possibility for creating artificial biological cells with this method. In the water/triolein and lecithin system, when the amount of oil was decreased on the permeate side, polygon or fiber cell types were created, and each cell contacted its neighbors across a thin oil layer like a biological tissue.

Key words

Emulsification emulsion instrumentation microchannel microprocessing visualization 


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Copyright information

© AOCS Press 1997

Authors and Affiliations

  • Takahiro Kawakatsu
    • 1
  • Yuji Kikuchi
    • 1
  • Mitsutoshi Nakajima
    • 1
  1. 1.National Food Research Institute, Ministry of Agriculture, Forestry and FisheriesIbarakiJapan

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