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Changes in phospholipid bilayers caused by sodium dodecyl sulfate/nonionic surfactant mixtures

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Journal of the American Oil Chemists' Society

Abstract

The interaction of mixtures of sodium dodecyl sulfate (SDS) and oxyethylenated nonylphenol (30 mol of ethylene oxide) [NP(EO)30] with phosphatidylcholine liposomes was investigated. Permeability alterations were detected as a change in 5(6)-carboxyfluorescein (CF) released from the interior of vesicles, and bilayer solubilization was measured as a decrease in the static light scattered by liposome suspensions. Three parameters were described as the effective surfactant/lipid molar ratios (Re) at which the surfactant system: (i) resulted in 50% CF release (Re 50%CF); (ii) saturated the liposomes (Re SAT); (iii) led to complete solubilization of these structures (Re SOL). The corresponding surfactant partition coefficients (K 50%CF, K SAT, and K SOL) were determined from these parameters. The free surfactant concentrations S W were lower than the mixed surfactant critical micellar concentration at subsolubilizing levels, whereas they remained similar to these values during saturation and solubilization of bilayers. Although the Re values increased linearly as the mole fraction of the SDS rose (X SDS), the K parameters showed maximum values at X SDS 0.6 for K 50%CF and approximately at X SDS 0.2 for K SAT and K SOL, respectively. Thus, the lower the surfactant contribution in the surfactant/lipid system, the higher the X SDS at which the maximum bilayer/water partitioning of added mixed surfactant systems occurred. As a consequence, the influence of SDS in this partition appears to be more significant at the sublytic level (monomeric effect), whereas the influence of NP(EO)30 seems to be greater during saturation and solubilization of liposomes via formation of mixed micelles.

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de la Maza, A., Parra, J.L. Changes in phospholipid bilayers caused by sodium dodecyl sulfate/nonionic surfactant mixtures. J Amer Oil Chem Soc 74, 9–17 (1997). https://doi.org/10.1007/s11746-997-0112-2

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  • DOI: https://doi.org/10.1007/s11746-997-0112-2

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