Journal of the American Oil Chemists' Society

, Volume 74, Issue 4, pp 413–418 | Cite as

Fractionation of squalene from amaranth seed oil

  • H. Sun
  • D. Wiesenborn
  • K. Tostenson
  • J. Gillespie
  • P. Rayas-Duarte


Amaranth seed oil was fractionated in a bench-scale short-path distillation unit to obtain fractions rich in squalene. Fractionations were conducted with degummed amaranth oil, alkali-refined amaranth oil, and simulated amaranth oil. Squalene concentration was increased about sevenfold with a squalene recovery of 76.0% in the distillate when degummed amaranth oil was fractionated at 180°C and 3 mtorr vacuum. Free fatty acids codistilled with squalene, lowering the squalene content of the distillate, and resulted in a semisolid distillate at room temperature. Alkali-refining was subsequently used to reduce the free fatty acid content before fractionation. A simulated oil (7% squalene/93% soybean oil) and alkali-refined amaranth oil were fractionated at three temperatures (160, 170, and 180°C) and five vacuum settings (10, 100, 200, 400, and 600 mtorr). The highest squalene recoveries from simulated oil and alkali-refined amaranth oil were 73.4 and 67.8%, respectively, both at 180°C and 100 mtorr, which translates to 12.1-and 9.2-fold increases in squalene concentration, respectively. The squalene recovery of the alkali-refined amaranth oil at 180°C was not significantly different at 10 mtorr vs. 100 mtorr. The results of this study can be used as a component to assess the economic feasibility of fractionating amaranth seed for starch, oil, meal, and squalene.

Key words

Alkali-refining amaranth oil distillation fractionation short-path distillation squalene squalene content squalene recovery 


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  1. 1.
    Breene, W.M., Food Use of Grain Amaranth, Cereal Foods World 36:426–430 (1991).Google Scholar
  2. 2.
    Lehmann, J.W., The Potential of Grain Amaranth in the 1990s and Beyond, Proceedings of the Fourth National Symposium: Perspectives on Production Processing and Marketing, Minnesota Extension Service, University of Minnesota, Minneapolis, MN, pp. 1–18 (1990).Google Scholar
  3. 3.
    Auguet, C.A., A New Source of Squalane, Drug Cosmet. Ind., November, 51–53, 82 (1988).Google Scholar
  4. 4.
    Bondioli, P., C. Mariani, A. Lanzani, E. Fedli, and A. Muller, Squalene Recovery from Olive Oil Deodorizer Distillates, J. Am. Oil Chem. Soc. 70:763–766 (1993).CrossRefGoogle Scholar
  5. 5.
    Becker, R., E.L. Wheeler, K. Lorenz, A.E. Stafford, O.K. Grosjean, A.A. Betschart, and R.M. Saunders, A Compositional Study of Amaranth Grain, J. Food Sci. 46:1175–1180 (1981).CrossRefGoogle Scholar
  6. 6.
    Sun, H., D. Wiesenborn, P. Rayas-Duarte, A. Mohamed, and K. Hagen, Bench-Scale Processing of Amaranth Seed for Oil, J. Am. Oil Chem. Soc. 72:1551–1555 (1995).CrossRefGoogle Scholar
  7. 7.
    Lyon, C.K., and R. Becker, Extraction and Refining of Oil from Amaranth Seed, Ibid.:233–236 (1987).CrossRefGoogle Scholar
  8. 8.
    Lanzani, A., P. Bondioli, C. Mariani, L. Folegatti, S. Venturini, E. Fedeli, and P. Barreteau, A New Short-Path Distillation System Applied to the Reduction of Cholesterol in Butter and Lard, Ibid.:609–614 (1994).CrossRefGoogle Scholar
  9. 9.
    Official Methods and Recommended Practices of the American Oil Chemists’ Society, 4th edn., American Oil Chemists’ Society, Champaign, 1989, Methods Ca 5a-40.Google Scholar
  10. 10.
    Kates, M., Techniques of Lipidology, Isolation, Analysis and Identification of Lipids, 2nd edn., Elsevier, New York, pp. 326–327 (1986).Google Scholar
  11. 11.
    Statistical Analysis System. SAS/STAT User’s Guide, Version 6, 4th edn., Vol. 2, SAS Institute, Cary, NC, 1990.Google Scholar
  12. 12.
    Budavari, S., The Merck Index, 11th edn., Merck & Co., Inc., Rahway, NJ, 1989.Google Scholar
  13. 13.
    Snyder, H.E., and T.W. Kwon, Soybean Utilization, Van Nostrand Reinhold Company, New York, 1987, pp. 37, 106.Google Scholar

Copyright information

© AOCS Press 1997

Authors and Affiliations

  • H. Sun
    • 2
  • D. Wiesenborn
    • 2
    • 1
  • K. Tostenson
    • 2
  • J. Gillespie
    • 1
  • P. Rayas-Duarte
    • 1
  1. 1.Department of Cereal ScienceNorth Dakota State UniversityFargo
  2. 2.Agricultural and Biosystems Engineering DepartmentNorth Dakota State UniversityFargo

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