Journal of the American Oil Chemists' Society

, Volume 94, Issue 4, pp 511–517 | Cite as

Gas Chromatographic Separation and Identification of Jacaric and Punicic 2-Ethyl-1-Hexyl Esters

  • Siriluck Pojjanapornpun
  • Kornkanok Aryusuk
  • Narumon Jeyashoke
  • Supathra Lilitchan
  • Kanit Krisnangkura
Original Paper

Abstract

The present study demonstrates the separation of a critical pair of conjugated linolenic acid (CLN) isomers—jacaric acid (JA; c8, t10, c12-18:3) and punicic acid (PA; c9, t11, c13-18:3)—on a 60-m conventional Supelcowax 10 column. The alkyl esters of different alcohols (C1–C8) of JA and PA were prepared and analyzed isothermally at 220, 230 and 240 °C. The adequacy of their separation was determined from the separation factors (α) and peak resolutions (Rs). Acceptable resolution (Rs = 1.01) of JA and PA was obtained with their 2-ethyl-1-hexyl ester derivatives at a column temperature of 230 °C. In addition, the Gibbs energy of transfer from solution to gas of the three double bonds \((\Delta_{\text{sln}}^{\text{g}} G_{\text{u}}\)) could be used to describe the interactions of the double bond with the stationary phase. Characterization of 2-ethyl-1-hexyl esters of Jacaranda mimosifolia seed oil at 230 °C demonstrates that the oil contains JA and α- and β-calendic acid as a CLN without the presence of PA. The results suggested that JA could be resolved from PA on a 60-m Supelcowax 10 column as the ethyl hexyl ester.

Keywords

Conjugated linolenic acid 2-Ethyl-1-hexyl ester GC Jacaranda seed oil Jacaric acid Punicic acid 

Supplementary material

11746_2017_2965_MOESM1_ESM.xlsx (15 kb)
Supplementary material 1 (XLSX 14 kb)

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Copyright information

© AOCS 2017

Authors and Affiliations

  • Siriluck Pojjanapornpun
    • 1
  • Kornkanok Aryusuk
    • 1
  • Narumon Jeyashoke
    • 1
  • Supathra Lilitchan
    • 2
  • Kanit Krisnangkura
    • 1
  1. 1.Division of Biochemical Technology, School of Bioresources and TechnologyKing Mongkut’s University of Technology ThonburiBangkokThailand
  2. 2.Department of Nutrition, Faculty of Public HealthMahidol UniversityBangkokThailand

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