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Determination of Fatty Acid Profiles and Esterase Activities in the Gills and Gonads of Vimba vimba (L., 1758)

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

The main purpose of this study was to evaluate the gills and gonads of Vimba vimba in terms of fatty acid composition, esterase activity, and esterase band patterns by native-PAGE method. In this context, C16:0 and C18:1n-9 were the most abundant fatty acids in the TLs, NLs, and the PLs fractions of both tissues. EPA (C20:5n-3) and DHA (C22:6n-3) levels were higher in the gills than in the gonads. MUFAs were the main fatty acid groups in the TLs, NLs, and PLs of both tissues. Gills had higher n-3/n-6 ratios when compared with the gonads, with the exception of the PLs. TLs of the gonads had the highest AI and TI values. A concentration of 1 mM Na-taurocholate increased the esterase activity of the gonads. Other chemicals having inhibition and surface active effects resulted in decreases in the esterase activities at concentrations of 1, 5, and 10 mM. In total, nine esterase bands were determined in both tissues. Four bands were shared by both the gills and gonads of V.vimba. These shared bands could be responsible for the similar esterase activities in both tissues. Gills and gonads of V. vimba have substantial amounts of fatty acids and esterase isozymes. More detailed studies are required to understand their industrial and nutritional uses.

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Abbreviations

AI:

Atherogenic Index

ARA:

Arachidonic acid

DHA:

Docosahexaenoic acid

EPA:

Eicosapentaenoic acid

FAME:

Fatty acid methyl ester

MUFA:

Monounsaturated fatty acid

n-3 PUFA:

Omega 3 polyunsaturated fatty acid

NLs:

Neutral lipids

PAGE:

Polyacrylamide gel electrophoresis

SFA:

Saturated fatty acid

PLs:

Polar lipids

TI:

Thrombogenicity Index

TLs:

Total lipids

References

  1. Sargent J, Bell G, McEvoy L, Tocher D, Estevez A (1999) Recent developments in the essential fatty acid nutrition of fish. Aquaculture 177:191–199

    Article  CAS  Google Scholar 

  2. Memon NN, Talpur FN, Bhanger MI, Balouch A (2011) Changes in fatty acid composition in muscle of three farmed carp fish species (Labeo rohita, Cirrhinus mrigala, Catla catla) raised under the same conditions. Food Chem 126:405–410

    Article  CAS  Google Scholar 

  3. Lauritzen L, Hansen HS, Jorgensen MH, Michaelsen KF (2001) The essentiality of long chain n-3 fatty acids in relation to development and function of the brain and retina. Prog Lipid Res 40:1–94

    Article  CAS  Google Scholar 

  4. Navarro-Garcia G, Gonzalez-Felix ML, Marquez-Farias F, Bringas-Alvarado L, Perez-Velazquez M, Montoya-Laos JM, Moreno-Silva B (2014) Lipid content and fatty acid composition of the liver from the rajiforms Urotrygon chilensis, Urobatis halleri, Rhinobatos glaucostigma, Rhinoptera steindachneri and Dasyatis dipeteura captured in Sinaloa, Mexico. Int Food Res J 21(1):229–235

    CAS  Google Scholar 

  5. Cakmak YS, Zengin G, Guler GO, Aktumsek A, Ozparlak H (2012) Fatty acid composition and Ω3/Ω6 ratios of the muscle lipids of six fish species in Sugla Lake, Turkey. Arch Biol Sci Belgrade 64(2):471–477

    Article  Google Scholar 

  6. Uysal K, Bülbül M, Dönmez M, Seçkin AK (2008) Changes in some components of the muscle lipids of three freshwater fish species under natural extreme cold and temperate conditions. Fish Physiol Biochem 34:455–463

    Article  CAS  Google Scholar 

  7. Görgün S, Akpınar N, Dirican S (2014) A comparative study on the fatty acid profiles of total lipid, neutral and polar lipids in the liver and muscle of Capoeta sieboldii (Steindachner, 1864) and Capoeta baliki (Turan, Kottelat, Ekmekçi, İmamoğlu, 2006) from Tödürge Lake (Sivas, Turkey). Acta Alim 43(1):170–181

    Article  Google Scholar 

  8. Tocher DR, Bendiksen EA, Campbell PJ, Bell JG (2008) The role of phospholipids in nutrition and metabolism of teleost fish. Aquaculture 280:21–34

    Article  CAS  Google Scholar 

  9. Gonçalves LU, Parisi G, Bonelli A, Sussel FR, Viegas EMM (2014) The fatty acid composition of total, neutral and polar lipids in wild and farmed lambari (Atyanax altiparanae) (Garutti & Britski, 2000) broodstock. Aquacult Res 45:195–203

    Article  Google Scholar 

  10. Beisson F, Tiss A, Riviere C, Verger R (2000) Methods for lipase detection and assay: a critical review. Eur J Lipid Sci Technol 102(2):133–153. doi:10.1002/(SICI)1438-9312(200002)102:2<133::AID-EJLT133>3.0.CO;2-X

    Article  CAS  Google Scholar 

  11. Gilham D, Lehner R (2005) Techniques to measure lipase and esterase activity in vitro. Methods 36:139–147

    Article  CAS  Google Scholar 

  12. Fahmy AS, Abo-Zeid AZ, Mohamed TM, Ghanem HM, Borai IH, Mohamed SA (2008) Characterization of esterases from Cucurbita pepo cv. “Eskandrani”. Bioresour Technol 99:437–443

    Article  CAS  Google Scholar 

  13. Bollag DM, Rozycki MD, Edelstein SJ (1996) Protein methods. Wiley, New York, pp 57–82

    Google Scholar 

  14. Aravindan R, Anbumathi P, Viruthagiri T (2007) Lipase application in food industry. Indian J Biotechnol 6:141–158

    CAS  Google Scholar 

  15. Okgerman HC, Yardımcı CH, Dorak Z, Yılmaz N (2013) Feeding ecology of vimba (Vimba vimba L., 1758) in terms of size groups and seasons in Lake Sapanca, northwestern Anatolia. Turk J Zool 37:288–297

    Google Scholar 

  16. Kalyoncu L, Kıssal S, Aktumsek A (2009) Seasonal changes in the total fatty acid composition of vimba, Vimba vimba tenella (Nordmann, 1840) in Eğirdir Lake, Turkey. Food Chem 116:728–730

    Article  CAS  Google Scholar 

  17. Görgün S, Akpınar N, Zengin G, Akpınar MA, Günlü A, Güler GO, Aktümsek A (2013) Determination of fatty acid profiles of total, neutral, and polar lipids in different tissues of Vimba vimba (L., 1758) from Eğirdir Lake (Isparta, Turkey). Turk J Zool 37:627–634

    Article  Google Scholar 

  18. Folch J, Less M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  Google Scholar 

  19. Kozlova TA, Khotimchenko SV (2000) Lipids and fatty acids of two pelagic cottoid fishes (Comephorus spp) endemic to Lake Baikal. Comp Biochem Phys B 126:477–485

    Article  CAS  Google Scholar 

  20. Moss CW, Lambert MA, Mervin WH (1974) Comparison of rapid methods for analysis of bacterial fatty acids. Appl Microbiol 28:80–85

    CAS  Google Scholar 

  21. Ulbricht TLV, Southgate DAT (1991) Coronary heart disease: seven dietary factors. Lancet 338:985–992

    Article  CAS  Google Scholar 

  22. Ülker S, Özel A, Çolak A, Alpay Karaoğlu Ş (2011) Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil. Turk J Biol 35:543–550

    Google Scholar 

  23. Akpınar MA, Görgün S, Akpınar AE (2009) A comparative analysis of the fatty acid profiles in the liver and muscles of male and female Salmo trutta macrostigma. Food Chem 112:6–8

    Article  Google Scholar 

  24. Jankowska B, Zakes Z, Zmijewski T, Szczepkowski M (2010) Fatty acid profile of muscles, liver and mesenteric fat in wild and reared perch (Perca fluviatilis L.). Food Chem 118:764–768

    Article  CAS  Google Scholar 

  25. Valfre F, Caprino F, Turchini GM (2003) The health benefit of seafood. Vet Res Commun 27:507–512

    Article  Google Scholar 

  26. Grigorakis K, Fountoulaki E, Vasilaki A, Mittakos I, Nathanailides C (2011) Lipid quality and filleting yield of reared meagre (Argyrosomus regius). Int J Food Sci Tech 46:711–716

    Article  CAS  Google Scholar 

  27. Görgün S, Akpınar MA (2012) Purification and characterization of lipase from the liver of carp, Cyprinus carpio L. (1758), living in Lake Tödürge (Sivas, Türkiye). Turk J Fish Aquat Sc 12:207–215

    Google Scholar 

  28. Kurtovic I, Marshall SN, Zhao X, Simpson BK (2010) Purification and properties of digestive lipases from Chinook salmon (Oncorhynchus tshawytscha) and New Zealand hoki (Macruronus novaezelandiae). Fish Physiol Biochem 36(4):1041–1060

    Article  CAS  Google Scholar 

  29. Lima VMG, Krieger N, Mitchell DA, Fontana JD (2004) Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents. Biochem Eng J 18(1):65–71

    Article  CAS  Google Scholar 

  30. Leticia A-G, Gerardo G-B (2008) Determination of esterase activity and characterization of cholinesterases in the reef fish Haemulon plumieri. Ecotox Environ Safe 71:787–797

    Article  CAS  Google Scholar 

  31. Shahjanan RM, Karim A, Begum RA, Alam MS, Begum A (2008) Tissue specific esterase isozyme banding pattern in the Nile Tilapia (Oreochromis niloticus). Univ J Zool Rajshahi Univ 27:1–5

    Google Scholar 

  32. Begum RA, Rahman DT, Rashid MA, Alam MS, Shahjanan RM (2012) Comparison of esterase isozyme variability in some selected tissues of the Asian and African catfishes (Siluriformes: Clariidae). Bangladesh J Zool 40(1):43–50

    Google Scholar 

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Acknowledgments

We thank Dr. Ali Günlü (Muğla Sıtkı Koçman University) for providing the fish samples. We also thank Michelle J Towers (Cumhuriyet University) for the English edition of the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Cumhuriyet University, 58140, Sivas, Turkey

    Salih Görgün

  2. Department of Biology, Faculty of Science, Selçuk University, 42075, Konya, Turkey

    Gökhan Zengin

Authors
  1. Salih Görgün
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  2. Gökhan Zengin
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Correspondence to Salih Görgün.

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Görgün, S., Zengin, G. Determination of Fatty Acid Profiles and Esterase Activities in the Gills and Gonads of Vimba vimba (L., 1758). J Am Oil Chem Soc 92, 353–360 (2015). https://doi.org/10.1007/s11746-015-2602-y

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  • DOI: https://doi.org/10.1007/s11746-015-2602-y

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