The Effect of Different Cold Storage Conditions on the Compositions of Extra Virgin Olive Oil

Abstract

Storage conditions can affect the stability and quality of extra virgin olive oil (EVOO). While many studies have reported the influence of high temperature and light exposure during storage, little is known on the influence of the cold storage. The aim of this study was to evaluate the effect of different storage conditions (25, 4.5 and −27 °C) on the various compositions of EVOO and to determine if cold storage will prolong shelf-life by retarding hydrolysis and oxidation. The changes of quality indices (FFA, PV, and UV) and natural antioxidants such as α-tocopherol and phenolic compounds were evaluated periodically during storage. The characterization and quantification of phenolics were achieved by ultra-performance liquid chromatography—diode array detector (UPLC-DAD). In addition, 1, 2-diacylglycerols (DAGs), pyropheophytin A (PPP) were measured to indicate thermal degradation during storage.

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References

  1. 1.

    Boskou D (1996) Olive oil: chemistry and technology. AOCS Press, Champaign

    Google Scholar 

  2. 2.

    Mulinacci N, Ieri F, Ignesti G, Romani A, Michelozzi M, Creti D, Innocenti M, Calamai L (2013) The freezing process helps to preserve the quality of extra virgin olive oil over time: a case study up to 18 months. Food Res Int 54:2008–2015

    CAS  Article  Google Scholar 

  3. 3.

    Cicerale S, Lucas LJ, Keast RSJ (2012) Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Curr Opin Biotechnol 23:129–135

    CAS  Article  Google Scholar 

  4. 4.

    de Lorgeril M, Renaud S, Salen P, Monjaud I, Mamelle N, Martin JL, Guidollet J, Touboul P, Delaye J (1994) Mediterranean alpha-linolenic acid-rich diet in secondary prevention of coronary heart disease. Lancet 343:1454–1459

    Article  Google Scholar 

  5. 5.

    Sánchez-Taínta A, Estruch R, Bulló M, Corella D, Gómez-Gracia E, Fiol M, Algorta J, Covas M-I, Lapetra J, Zazpe I, Ruiz-Gutiérrez V, Ros E, Martínez-González MA (2008) Adherence to a Mediterranean-type diet and reduced prevalence of clustered cardiovascular risk factors in a cohort of 3204 high-risk patients. Eur J Cardiovasc Prev Rehabil 15:589–593

    Article  Google Scholar 

  6. 6.

    Solfrizzi V, Panza F, Torres F, Mastroianni F, Del Parigi A, Venezia A, Capurso A (1999) High monounsaturated fatty acids intake protects against age-related cognitive decline. Neurology 52:1563–1569

    CAS  Article  Google Scholar 

  7. 7.

    Fitó M, Guxens M, Corella D et al (2007) Effect of a traditional mediterranean diet on lipoprotein oxidation: a randomized controlled trial. Arch Intern Med 167:1195–1203

    Article  Google Scholar 

  8. 8.

    Fitó M, Cladellas M, de la Torre R, Martí J, Alcántara M, Pujadas-Bastardes M, Marrugat J, Bruguera J, López-Sabater MC, Vila J, Covas MI (2005) Antioxidant effect of virgin olive oil in patients with stable coronary heart disease: a randomized, crossover, controlled, clinical trial. Atherosclerosis 181:149–158

    Article  Google Scholar 

  9. 9.

    Perez-Jimenez F, de Cienfuegos G, Badimon L (2005) International conference on the healthy effect of virgin olive oil. Eur J Clin Invest 35:421–424

    CAS  Article  Google Scholar 

  10. 10.

    Caponio F, Bilancia MT, Pasqualone A, Sikorska E, Gomes T (2005) Influence of the exposure to light on extra virgin olive oil quality during storage. Eur Food Res Technol 221:92–98

    CAS  Article  Google Scholar 

  11. 11.

    Kiritsakis AK (1998) Olive oil from the tree to the table, 2nd edn. Food & Nutrition Press Inc, Trumbull

    Google Scholar 

  12. 12.

    Samaniego-Sanchez C, Oliveras-Lopez MJ, Quesada-Granados JJ, Villalon-Mir M, Serrana HLG (2012) Alterations in picual extra virgin olive oils under different storage conditions. Eur J Lipid Sci Technol 114:194–204

    CAS  Article  Google Scholar 

  13. 13.

    Servili M, Montedoro G (2002) Contribution of phenolic compounds to virgin olive oil quality. Eur J Lipid Sci Technol 104:602–613

    CAS  Article  Google Scholar 

  14. 14.

    Omar SH (2010) Oleuropein in olive and its pharmacological effects. Sci Pharm 78:22

    Google Scholar 

  15. 15.

    Coni E, Di Benedetto R, Di Pasquale M, Masella R, Modesti D, Mattei R, Carlini EA (2000) Protective effect of oleuropein, an olive oil biophenol, on low density lipoprotein oxidizability in rabbits. Lipids 35:45–54

    CAS  Article  Google Scholar 

  16. 16.

    Briante R, La Cara F, Febbraio F, Barone R, Piccialli G, Carolla R, Mainolfi P, De Napoli L, Patumi M, Fontanazza G, Nucci R (2000) Hydrolysis of oleuropein by recombinant beta-glycosidase from hyperthermophilic archaeon Sulfolobus solfataricus immobilised on chitosan matrix. J Biotechnol 77:275–286

    CAS  Article  Google Scholar 

  17. 17.

    Tuck KL, Hayball PJ (2002) Major phenolic compounds in olive oil: metabolism and health effects. J Nutr Biochem 13:636–644

    CAS  Article  Google Scholar 

  18. 18.

    Blekas G, Tsimidou M, Boskou D (1995) Contribution of alpha-tocopherol to olive oil stability. Food Chem 52:289–294

    CAS  Article  Google Scholar 

  19. 19.

    Perez-Camino MC, Moreda W, Cert A (2001) Effects of olive fruit quality and oil storage practices on the diacylglycerol content of virgin olive oils. J Agric Food Chem 49:699–704

    CAS  Article  Google Scholar 

  20. 20.

    Gimeno E, Castellote AI, Lamuela-Raventos RM, de la Torre MC, Lopez-Sabater MC (2000) Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography. J Chromatogr A 881:251–254

    CAS  Article  Google Scholar 

  21. 21.

    Mateos R, Espartero JL, Trujillo M, Ríos JJ, León-Camacho M, Alcudia F, Cert A (2001) Determination of phenols, liquid chromatography with diode array ultraviolet detection. J Agric Food Chem 49:2185–2192

    CAS  Article  Google Scholar 

  22. 22.

    Carrasco-Pancorbo A, Cerretani L, Bendini A, Segura-Carretero A, Del Carlo M, Gallina-Toschi T, Lercker G, Compagnone D, Fernandez-Gutierrez A (2005) Evaluation of the antioxidant capacity of individual phenolic compounds in virgin olive oil. J Agric Food Chem 53:8918–8925

    CAS  Article  Google Scholar 

  23. 23.

    Zhu H, Clegg MS, Shoemaker CF, Wang SC (2013) Characterization of diacylglycerol isomers in edible oils using gas chromatography–ion trap electron ionization mass spectrometry. J Chromatogr A 1304:194–202

    CAS  Article  Google Scholar 

  24. 24.

    Ayton J, Mailer R, Graham K (2012) The effect of storage conditions on extra virgin olive oil quality. http://static.oliveoiltimes.com/library/Olive-Oil-Storage-Conditions.pdf. Accessed April 2012

  25. 25.

    Vekiari SA, Papadopoulou R, Koutsaftakis A (2002) Comparison of different olive oil extraction systems and the effect of storage conditions on the quality of the virgin olive oil. Grasas Aceites 53:324–329

    CAS  Google Scholar 

  26. 26.

    Pristouri G, Badeka A, Kontominas MG (2010) Effect of packaging material headspace, oxygen and light transmission, temperature and storage time on quality characteristics of extra virgin olive oil. Food Control 21:412–418

    CAS  Article  Google Scholar 

  27. 27.

    Gutierrez F, Fernandez JL (2002) Determinant parameters and components in the storage of virgin olive oil. Prediction of storage time beyond which the oil is no longer of “extra” quality. J Agric Food Chem 50:571–577

    CAS  Article  Google Scholar 

  28. 28.

    Marmesat S, Morales A, Velasco J, Ruiz-Mendez MV, Dobarganes MC (2009) Relationship between changes in peroxide value and conjugated dienes during oxidation of sunflower oils with different degree of unsaturation. Grasas Aceites 60:155–160

    CAS  Article  Google Scholar 

  29. 29.

    Clodoveo ML, Delcuratolo D, Gomes T, Colelli G (2007) Effect of different temperatures and storage atmospheres on Coratina olive oil quality. Food Chem 102:571–576

    CAS  Article  Google Scholar 

  30. 30.

    Allouche Y, Jimenez A, Gaforio JJ, Uceda M, Beltran G (2007) How heating affects extra virgin olive oil quality indexes and chemical composition. J Agric Food Chem 55:9646–9654

    CAS  Article  Google Scholar 

  31. 31.

    Servili M, Esposto S, Fabiani R, Urbani S, Taticchi A, Mariucci F, Selvaggini R, Montedoro GF (2009) Phenolic compounds in olive oil: antioxidant, health and organoleptic activities according to their chemical structure. Inflammopharmacology 17:76–84

    CAS  Article  Google Scholar 

  32. 32.

    Servili M, Selvaggini R, Esposto S, Taticchi A, Montedoro G, Morozzi G (2004) Health and sensory properties of virgin olive oil hydrophilic phenols: agronomic and technological aspects of production that affect their occurrence in the oil. J Chromatogr A 1054:113–127

    CAS  Article  Google Scholar 

  33. 33.

    Baldioli M, Servili M, Perretti G, Montedoro GF (1996) Antioxidant activity of tocopherols and phenolic compounds of virgin olive oil. J Am Oil Chem Soc 73:1589–1593

    CAS  Article  Google Scholar 

  34. 34.

    Servili M, Baldioli M, Selvaggini R, Macchioni A, Montedoro G (1999) Phenolic compounds of olive fruit: one- and two-dimensional nuclear magnetic resonance characterization of nuzhenide and its distribution in the constitutive parts of fruit. J Agric Food Chem 47:12–18

    CAS  Article  Google Scholar 

  35. 35.

    Cinquanta L, Esti M, LaNotte E (1997) Evolution of phenolic compounds virgin olive oil during storage. J Am Oil Chem Soc 74:1259–1264

    CAS  Article  Google Scholar 

  36. 36.

    Gomez-Alonso S, Mancebo-Campos V, Salvador MD, Fregapane G (2007) Evolution of major and minor components and oxidation indices of virgin olive oil during 21 months storage at room temperature. Food Chem 100:36–42

    CAS  Article  Google Scholar 

  37. 37.

    Cerretani L, Bendini A, Biguzzi B, Lercker G (2005) Freezing storage can affect the oxidative stability of not filtered extra-virgin olive oils. J Commod Sci Technol Qual 44:3–15

    CAS  Google Scholar 

  38. 38.

    Bendini A, Cerretani L, Salvador MD, Fregapane G, Lercker G (2009) Stability of the sensory quality of virgin olive oil during storage: an overview. Italian J Food Sci 21:389–406

    CAS  Google Scholar 

  39. 39.

    Gallardo-Guerrero L, Gandul-Rojas B, Roca M, Minguez-Mosquera I (2005) Effect of storage on the original pigment profile of Spanish virgin olive oil. J Am Oil Chem Soc 82:33–39

    CAS  Article  Google Scholar 

  40. 40.

    Aparicio-Ruiz R, Roca M, Gandul-Rojas B (2012) Mathematical model to predict the formation of pyropheophytin a in virgin olive oil during storage. J Agric Food Chem 60:7040–7049

    CAS  Article  Google Scholar 

  41. 41.

    Cossignani L, Luneia R, Damiani P, Simonetti MS, Riccieri R, Tiscornia E (2007) Analysis of isomeric diacylglycerolic classes to evaluate the quality of olive oil in relation to storage conditions. Eur Food Res Technol 224:379–383

    CAS  Article  Google Scholar 

  42. 42.

    Rastrelli L, Passi S, Ippolito F, Vacca G, De Simone F (2002) Rate of degradation of alpha-tocopherol, squalene, phenolics, and polyunsaturated fatty acids in olive oil during different storage conditions. J Agric Food Chem 50:5566–5570

    CAS  Article  Google Scholar 

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Acknowledgments

The authors are grateful to sensory scientist Sue Langstaff for her assistance with the duo-trio test and Dan Flynn for helpful discussions. We thank California Olive Ranch for the donation of oil samples and Agilent Technologies for their support of the UPLC.

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Correspondence to Selina C. Wang.

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Li, X., Zhu, H., Shoemaker, C.F. et al. The Effect of Different Cold Storage Conditions on the Compositions of Extra Virgin Olive Oil. J Am Oil Chem Soc 91, 1559–1570 (2014). https://doi.org/10.1007/s11746-014-2496-0

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Keywords

  • Extra virgin olive oil
  • Cold storage conditions
  • Oxidation
  • Antioxidants