Journal of the American Oil Chemists' Society

, Volume 90, Issue 6, pp 757–770

Rapid FT-NIR Analysis of Edible Oils for Total SFA, MUFA, PUFA, and Trans FA with Comparison to GC

  • Magdi M. Mossoba
  • Hormoz Azizian
  • Cynthia Tyburczy
  • John K. G. Kramer
  • Pierluigi Delmonte
  • Ali-Reza Fardin Kia
  • Jeanne I. Rader
Original Paper

DOI: 10.1007/s11746-013-2234-z

Cite this article as:
Mossoba, M.M., Azizian, H., Tyburczy, C. et al. J Am Oil Chem Soc (2013) 90: 757. doi:10.1007/s11746-013-2234-z


Declarations of the total content of trans fatty acids (FA) and saturated FA (SFA) are mandatory on food labels in the US and Canada. Gas chromatography (GC) has been the method of choice for the determination of FA composition. However, GC is time consuming and requires conversion of fats and oils to their FA methyl esters. In the present study, a recently published Fourier transform near-infrared (FT-NIR) spectroscopic procedure was applied to the rapid (<5 min) determination of total SFA, monounsaturated FA (MUFA), polyunsaturated FA (PUFA), and trans FA contents of 30 commercially available edible fats and oils. Good agreement was obtained between the GC and FT-NIR methods for the determination of total SFA, MUFA, and PUFA contents. Differences between the two methods were apparent for the determination of trans fat at trans fat levels <2 % of total fat. The analytical determinations of total SFA, MUFA, and PUFA contents for many of the oils examined differed from the respective values declared on the product labels. Our findings demonstrate that the FT-NIR procedure serves as a suitable alternative method for the rapid determination of total SFA, MUFA, PUFA and trans FA contents of neat vegetable oils.


Fats and oils Spectroscopy Lipid Chemistry/Lipid Analysis 



American Oil Chemist’s Society


Attenuated total reflection–Fourier transform mid-infrared


Covalent adduct chemical ionization tandem mass spectrometry


Conjugated linoleic acid


Electron impact MS


Fatty acid


FA methyl ester


Fourier transform near-infrared


Gas chromatography


Monounsaturated FA


Polyunsaturated FA


Saturated FA

Copyright information

© AOCS (outside the USA) 2013

Authors and Affiliations

  • Magdi M. Mossoba
    • 1
  • Hormoz Azizian
    • 2
  • Cynthia Tyburczy
    • 1
  • John K. G. Kramer
    • 3
  • Pierluigi Delmonte
    • 1
  • Ali-Reza Fardin Kia
    • 1
  • Jeanne I. Rader
    • 1
  1. 1.Food and Drug Administration, Center for Food Safety and Applied NutritionOffice of Regulatory ScienceCollege ParkUSA
  2. 2.NIR Technologies Inc.OakvilleCanada
  3. 3.Guelph Food Research Center, Agri-food CanadaGuelphCanada

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