Rapid FT-NIR Analysis of Edible Oils for Total SFA, MUFA, PUFA, and Trans FA with Comparison to GC

  • Magdi M. Mossoba
  • Hormoz Azizian
  • Cynthia Tyburczy
  • John K. G. Kramer
  • Pierluigi Delmonte
  • Ali-Reza Fardin Kia
  • Jeanne I. Rader
Original Paper

Abstract

Declarations of the total content of trans fatty acids (FA) and saturated FA (SFA) are mandatory on food labels in the US and Canada. Gas chromatography (GC) has been the method of choice for the determination of FA composition. However, GC is time consuming and requires conversion of fats and oils to their FA methyl esters. In the present study, a recently published Fourier transform near-infrared (FT-NIR) spectroscopic procedure was applied to the rapid (<5 min) determination of total SFA, monounsaturated FA (MUFA), polyunsaturated FA (PUFA), and trans FA contents of 30 commercially available edible fats and oils. Good agreement was obtained between the GC and FT-NIR methods for the determination of total SFA, MUFA, and PUFA contents. Differences between the two methods were apparent for the determination of trans fat at trans fat levels <2 % of total fat. The analytical determinations of total SFA, MUFA, and PUFA contents for many of the oils examined differed from the respective values declared on the product labels. Our findings demonstrate that the FT-NIR procedure serves as a suitable alternative method for the rapid determination of total SFA, MUFA, PUFA and trans FA contents of neat vegetable oils.

Keywords

Fats and oils Spectroscopy Lipid Chemistry/Lipid Analysis 

Abbreviations

AOCS

American Oil Chemist’s Society

ATR-FTIR

Attenuated total reflection–Fourier transform mid-infrared

CACI-MS/MS

Covalent adduct chemical ionization tandem mass spectrometry

CLA

Conjugated linoleic acid

EI-MS

Electron impact MS

FA

Fatty acid

FAME

FA methyl ester

FT-NIR

Fourier transform near-infrared

GC

Gas chromatography

MUFA

Monounsaturated FA

PUFA

Polyunsaturated FA

SFA

Saturated FA

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Copyright information

© AOCS (outside the USA) 2013

Authors and Affiliations

  • Magdi M. Mossoba
    • 1
  • Hormoz Azizian
    • 2
  • Cynthia Tyburczy
    • 1
  • John K. G. Kramer
    • 3
  • Pierluigi Delmonte
    • 1
  • Ali-Reza Fardin Kia
    • 1
  • Jeanne I. Rader
    • 1
  1. 1.Food and Drug Administration, Center for Food Safety and Applied NutritionOffice of Regulatory ScienceCollege ParkUSA
  2. 2.NIR Technologies Inc.OakvilleCanada
  3. 3.Guelph Food Research Center, Agri-food CanadaGuelphCanada

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