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Pro-Oxidant Effects of β-Carotene During Thermal Oxidation of Edible Oils

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

β-Carotene is one of the most important fat soluble pigments with well-known antioxidant and pro-vitamin A activity. It is used in industries as a food colorant and a source of vitamin A. The thermal induced degradation during processing of wide varieties of carotenoid-rich foods leads to color and properties losses. The thermal stability of edible oils is thus of great importance to food manufacturers. Corn oil, rapeseed, and sunflower oils were fortified with 50–300 μg/g of β-carotene and oxidized using a Rancimat apparatus (air flow rate 20 L/h) at 110 °C for 14 h. β-Carotene degradation was measured using high performance thin layer chromatography and confirmed by HPLC–DAD–MS. Triacylglycerols and polar compounds (PC) were determined using LC–ESI–MS. Results showed that most of the β-carotene was degraded during the first 5 h of the thermal oxidation. It was found that the addition of β-carotene produces significant effects (P < 0.05) on the peroxide index, free fatty acid values and radical scavenging activity of the three oils. Triacylglycerols containing high amounts of oleic acid show higher stability toward thermal oxidation and β-carotene treatment. Among the oils, rapeseed oil was the most stable oil in terms of the formation of polar compounds (PC), followed by corn oil, while sunflower oil was more prone to oxidation and thus higher amounts of PC were formed.

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References

  1. Paiva SAR, Russell RM (1999) β-Carotene and other carotenoids as antioxidants. J Am Coll Nutr 18:426–433

    CAS  Google Scholar 

  2. Black HS, Okotie-Eboh G, Gerguis J (2000) Diet potentiates the UV-carcinogenic response to beta-carotene. Nutr Cancer 37:173–178

    Article  CAS  Google Scholar 

  3. Goulson MJ, Warthesen JJ (1999) Stability and antioxidant activity of β-carotene in conventional and high oleic canola oil. J Food Sci 64:996–999

    Article  CAS  Google Scholar 

  4. Zeb A (2012) Thermal degradation of β-carotene in food oils. In: Preedy VR (ed) Vitamin A and carotenoids: chemistry, analysis, functions and effects. Royal Society of Chemistry, London, pp 129–141

    Chapter  Google Scholar 

  5. Britton G, Liaaen-Jensen S, Pfander H (2008) Special molecules, special properties. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids: natural functions. Birkhäuser, Basel, pp 1–6

    Chapter  Google Scholar 

  6. Lee EC, Min DB (1998) Quenching mechanism of β-carotene on the chlorophyll sensitized photo oxidation of soybean oil. J Food Sci 53:1894–1895

    Article  Google Scholar 

  7. Steenson DF, Min DB (2000) Effect of β-carotene and lycopene thermal degradation products on the oxidative stability of soybean oil. J Am Oil Chem Soc 77:1153–1160

    Article  CAS  Google Scholar 

  8. Zeb A, Murkovic M (2010) Analysis of triacylglycerols in refined edible oils by isocratic HPLC–ESI–MS. Eur J Lipid Sci Technol 112:1218–1228

    Article  CAS  Google Scholar 

  9. Manorama R, Brahmam GNV, Rukmini C (1996) Red palm oil as a source of β-carotene for combating vitamin A deficiency. Plant Food Hum Nutr 49:775–782

    Article  Google Scholar 

  10. Zeb A, Malook I (2009) Biochemical characterization of sea buckthorn (Hippophae rhamnoides L. spp. turkestanica) seed. Afr J Biotechnol 8:1625–1629

    CAS  Google Scholar 

  11. Zeb A (2011) Effects of β-carotene on the thermal oxidation of fatty acids. Afr J Biotechnol 10:15346–15352

    Article  CAS  Google Scholar 

  12. Minguez-Mosquera MI, Gandul-Rojas B, Gallardo-Guerrero ML (1992) Rapid method of quantification of chlorophylls and carotenoids in virgin olive oil by high-performance liquid chromatography. J Agric Food Chem 40:60–63

    Article  CAS  Google Scholar 

  13. Zeb A, Murkovic M (2010) High performance thin layer chromatographic method for monitoring the thermal degradation of β-carotene in sunflower oil. J Planar Chromatogr 23:35–39

    Article  CAS  Google Scholar 

  14. Perez-Galvez A, Rios JJ, Minguez-Mosquera MI (2005) Thermal degradation products formed from carotenoids during a heat-induced degradation process of paprika oleoresins (Capsicum annuum L.). J Agric Food Chem 53:4820–4826

    Article  CAS  Google Scholar 

  15. AOCS (1998) Official Methods and Recommended Practice. In: Firestone D (ed), 5th Edn. AOCS, Champaign

  16. Benakmoum A, Abbeddou S, Ammouche A, Kefalas P, Gerasopoulos D (2008) Valorisation of low quality edible oil with tomato peel waste. Food Chem 110:684–690

    Article  CAS  Google Scholar 

  17. IUPAC (1979) Standard methods for the analysis of oils, fats and derivatives, 6th edn. Pergamon Press, Oxford

    Google Scholar 

  18. Ramadan MF, Amer MMA, Sulieman ARM (2006) Correlation between physicochemical analysis and radical scavenging activity of vegetable oil blends as affected by frying of french fries. Eur J Lipid Sci Technol 108:670–678

    Article  CAS  Google Scholar 

  19. Zeb A, Murkovic M (2010) Characterization of the effects of β-carotene on the thermal oxidation of triacylglycerols using HPLC–ESI–MS. Eur J Lipid Sci Technol 112:844–851

    Article  CAS  Google Scholar 

  20. Henry LK, Catignani GL, Schwartz SJ (1998) Oxidative degradation kinetics of lycopene, lutein, and 9-cis and all-trans β-carotene. J Am Oil Chem Soc 75:823–829

    Article  CAS  Google Scholar 

  21. Hunter RF, Krakenberger RM (1947) The oxidation of β-carotene in solution by oxygen. J Chem Soc 1–4 (January)

  22. Yanishlieva NV, Marinova EM (2001) Stabilization of edible oils with natural antioxidants. Eur J Lipid Sci Technol 103:752–767

    Article  CAS  Google Scholar 

  23. Yanishlieva NV, Raneva VG, Marinova EM (2001) β-Carotene in sunflower oil oxidation. Grasas Aceitas 52:10–16

    CAS  Google Scholar 

  24. Bartee SD, Kim HJ, Min DB (2007) Effects of antioxidants on the oxidative stability of oils containing arachidonic, docosapentaenoic and docosahexaenoic acids. J Am Oil Chem Soc 84:363–368

    Article  CAS  Google Scholar 

  25. Shahidi F, Wanasundara UN (1997) Measurement of lipid oxidation and evaluation of antioxidant activity In: Natural antioxidants, chemistry, health effects and applications. AOCS Press, Champaign

  26. Lee JM, Chung H, Chang P, Lee JH (2007) Development of a method predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chem 103:662–669

    Article  CAS  Google Scholar 

  27. Park YW, Jeong MK, Park CU, Lee JH (2011) Distribution of triacylglycerols and fatty acids in soybean oil with thermal oxidation and methylene blue photosensitization. J Am Oil Chem Soc 88:373–380

    Article  CAS  Google Scholar 

  28. Zeb A, Murkovic M (2011) Carotenoids and triacylglycerols oxidation during thermal oxidation of refined olive oil. Food Chem 127:1584–1593

    Article  CAS  Google Scholar 

  29. Arroyo R, Cuesta C, Garrido-Polonio C, López-Varela S, Sánchez-Muniz FJ (1992) High-performance size-exclusion chromatographic studies on polar components formed in sunflower oil used for frying. J Am Oil Chem Soc 69:557–563

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The corresponding author (Dr. Alam Zeb) is grateful to the Higher Education Commission (HEC), Government of Pakistan for financial support for studying at Graz University of Technology, Graz, Austria and the Doctoral School of Bioscience and Biotechnology for financial support to present the results at the 1st European Food Congress in Ljubljana, Slovenia.

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Correspondence to Alam Zeb.

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Zeb, A., Murkovic, M. Pro-Oxidant Effects of β-Carotene During Thermal Oxidation of Edible Oils. J Am Oil Chem Soc 90, 881–889 (2013). https://doi.org/10.1007/s11746-013-2221-4

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  • DOI: https://doi.org/10.1007/s11746-013-2221-4

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