Skip to main content
Log in

Oil, Fatty Acid Profile and Karanjin Content in Developing Pongamia pinnata (L.) Pierre Seeds

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

Oil content, fatty acid composition and karanjin content were studied in developing pongamia seeds, at intervals of 3 weeks from 30 weeks after flowering up to 42 weeks. Three marked stages in seed development were observed at the early green pod stage, the middle half brown stage and the late dark brown stage. Significant variation in seed biomass, pod and seed characteristics were observed. A significant (P < 0.01) decrease in the moisture content of the seeds was observed during seed development. The oil content gradually increased from 32.06 to 36.53 % as the seed matured. A significant variation in fatty acid composition was detected across all stages of seed development. Palmitic acid (16:0) content marginally decreased from 11.81 to 10.18 %, while stearic acid (18:0) and linolenic acid (18:3) remained constant at all stages of seed maturity. A steady increase in oleic acid (18:1) content from 38.11 to 49.11 % was observed, while the linoleic acid (18:2) content decreased from 30.14 to 18.85 %. The iodine value increased, while the saponification number of oil decreased during seed development. The increase in karanjin content was steady. Seeds harvested after 42 week after flowering yielded the maximum oil with high oleic acid content which could be suitable for biodiesel production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

WAF:

Weeks after flowering

FAME:

Fatty acid methyl esters

DMRT:

Duncan multiple range test

MC:

Moisture content

References

  1. Muthu C, Ayyanar M, Raja N, Ignacimuthu S (2006) Medicinal plants used by traditional healers in Kanchipuram provenance of Tamil Nadu, India. J Ethnobiol Ethnomed 2:43

    Article  Google Scholar 

  2. Pavela R (2009) Effectiveness of some botanical insecticides against Spodoptera littoralis Boisduvala (Lepidoptera: Noctudiae), Myzus persicae Sulzer (Hemiptera: Aphididae) and Tetranychus urticae Koch (Acari: Tetranychidae). Plant Prot Sci 45(4):161–167

    CAS  Google Scholar 

  3. Anonymous (1969) Wealth of India: raw materials, publication and information directorate, Council of Scientific and Industrial Research, New Delhi, pp 206–211

  4. Naik M, Meher LC, Naik SN, Dasa LM (2008) Production of biodiesel from high free fatty acid karanj (Pongamia pinnata) oil. Biomass Bioenergy 32:354–357

    Article  CAS  Google Scholar 

  5. Birajdar S, Ramesh S, Chimkod V, Patil CS (2011) Phytochemical and physiochemical screening of Pongamia pinnata seeds. Int J Biotechnol appl 3(1):52–54

    Google Scholar 

  6. Yadav PP, Ahmad GA, Maurya R (2004) Furanoflavonoids from Pongamia pinnata fruits. Phytochem 65:429–442

    Article  Google Scholar 

  7. Majumdar D (2008) Unexploited botanical nitrification inhibitors prepared from karanja plant. Nat Prod Radiance 7(1):58–67

    Google Scholar 

  8. Chen Y, Wang Y, Zhou G, Li P, Zhang S (2008) Key mediators modulating TAG synthesis and accumulation in woody plants. Afr J Biotechnol 7(25):4742–4749

    Google Scholar 

  9. Deng X, Scarth R (1998) Temperature effects on fatty acid composition during development of low-linolenic oil seed rape (Brassica napus L.). J Am Oil Chem Soc 75(7):759–766

    Article  CAS  Google Scholar 

  10. Wiberg E, Banas A, Stymne S (1997) Fatty acid distribution and lipid metabolism in developing seeds of laurate producing rape (Brassica napus L.). Planta 203:341–348

    Article  CAS  Google Scholar 

  11. Cherif A, Sebei K, Boukhchina S, Kallel H, Belkacemi K, Arul J (2004) Kernel fatty acid and triacylglycerol composition for three almond cultivars during maturation. J Am Oil Chem Soc 81(10):901–905

    Article  CAS  Google Scholar 

  12. Ramos MJ, Fernandez CM, Caras A, Rodriguez L, Perez A (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresour Technol 100:261–268

    Article  CAS  Google Scholar 

  13. Knothe G (2005) Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process Technol 86:1059–1070

    Article  CAS  Google Scholar 

  14. ISTA (2005) International rules for seed testing. International Seed Testing Association, Bassersdorf

    Google Scholar 

  15. Kalayasiri P, Jayashke N, Krisnangkura K (1996) Survey of seed oils for use as diesel fuels. J Am Oil Chem Soc 73:471–474

    Article  CAS  Google Scholar 

  16. Panda AK, Sastry VRB, Kumar A, Saha SK (2006) Quantification of karanjin, Tannin and Trypsin Inhibitors in Raw and detoxified expeller and solvent extracted karanj (Pongamia glabra) cake. Asian Aust J Animal Sci 19(12):1776–1783

    CAS  Google Scholar 

  17. Panse VG, Sukhatme PV (1976) Statistical methods for agricultural workers. ICAR, New Delhi

    Google Scholar 

  18. Borek S, Pukacka S, Michalski K, Ratajczak L (2009) Lipid and protein accumulation in developing seeds of three lupin species: Lupinus luteus L., Lupinus albus L., and Lupinus mutabilis Sweet. J Exp Bot 60(12):3453–3466

    Article  CAS  Google Scholar 

  19. Crocker W, Barton LV (1957) Physiology of seeds: an introduction to the experimental study of seed and germination problems. Chronica Botanica Company, Waltham

    Google Scholar 

  20. Sorensen BM, Furukawa-Stoffer TL, Marshall KS, Page EK, Mir Z, Forster RJ, Weselake RJ (2005) Storage lipid accumulation and acyltransferase action in developing flaxseed. Lipids 40(10):1042–1049

    Article  Google Scholar 

  21. Ghassemi-Golezani K, Taijeh-Noori M, Oustan S, Moghaddan M, Rahmani SS (2010) Oil and protein accumulation in soybean grains under salinity stress. Not Sci Biol 2(2):64–67

    CAS  Google Scholar 

  22. Rahamatalla AB, Babiker EE, Krishna AG, El Tinay AH (2001) Changes in fatty acid composition during seed growth and physiochemical characteristics of oil extracted from four safflower cultivars. Plant Foods Hum Nutr 56:385–395

    Article  CAS  Google Scholar 

  23. Ohlrogge J, Browse J (1995) Lipid biosynthesis. Plant Cell 7:957–970

    CAS  Google Scholar 

  24. Haathurusingha S, Ashwath N, Subedi P (2010) Variation in oil content and fatty acid profile of Calophyllum inophyllum L. with fruit maturity and its implications on resultant biodiesel quality. Ind Crops Prod doi: 10.1016/j.indcrop.2010.12.026

  25. Berti MT, Johnson BL (2008) Physiological changes during seed development of cuphea. Field Crops Res 106:163–170

    Article  Google Scholar 

  26. Gecgel U, Demirci M, Esendal E, Tasan M (2007) Fatty acid composition of the oil from developing seeds of different varieties of safflower (Carthamus tinctorius L.). J Amer Oil Chem Soc 84:47–54

    Article  CAS  Google Scholar 

  27. Voelker T, Kinney AJ (2001) Variations in the biosynthesis of seed storage lipids. Annu Rev Plant Physiol Plant Mol Biol 52:335–361

    Article  CAS  Google Scholar 

  28. Bala M, Nag TN, Kumar S, Vyas M, Kumar A, Bhogal NS (2011) Proximate composition and fatty acid profile of Pongamia pinnata, a potential biodiesel crop. J Am Oil Chem Soc 88:559–562

    Article  CAS  Google Scholar 

  29. Wu Q, Liu T, Liu H, Zheng G (2009) Unsaturated fatty acid: metabolism, synthesis and gene regulation. Afr J Biotechnol 8(9):1782–1785

    CAS  Google Scholar 

  30. UNE-EN 14103 (2003) Fat and oil derivatives. Fatty acid methyl esters (FAME) Determination of ester and linolenic acid methyl ester contents

  31. Knothe G (2002) Structure indices in FA chemistry. How relevant is the iodine value? J Am Oil Chem Soc 79(9):847–854

    Article  CAS  Google Scholar 

  32. UNE-EN 14111 (2003) Fat and oil derivatives. Fatty acid methyl esters (FAME) determination of iodine value

  33. Mittelbach M (1996) Diesel fuel derived from vegetable oil VI specifications and quality control of biodiesel. Bioresour Technol 56:7–11

    Article  CAS  Google Scholar 

  34. Azam MM, Waris A, Nahar NM (2005) Prospects and potential of fatty acids methyl ester of some non-traditional seed oils for use as biodiesel in India. Biomass Bioenergy 29:293–302

    Article  Google Scholar 

  35. Lanna AC, Jose IC, Oliveira MGA, Barros EG, Moreira MA (2005) Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds. Brazil J Plant Physiol 17(2):213–222

    CAS  Google Scholar 

  36. Lung SC, Weselake RJ (2006) Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids 41(12):1073–1088

    Article  CAS  Google Scholar 

  37. Shirley BW (1998) Flavonoids in seeds and grains: physiological functions, agronomic importance and the genetics of biosynthesis. Seed Sci Res 8:415–422

    Article  CAS  Google Scholar 

  38. Stafford HA (1991) Flavonoid evolution: an enzymatic approach. Plant Physiol 96:680–685

    Article  CAS  Google Scholar 

  39. Treutter D (2006) Significance of flavonoids in plant resistance: a review. Environ Chem Lett 4:147–157

    Article  CAS  Google Scholar 

  40. Khatun A, Kabir G, Bhuiyan MAH (2009) Effect of harvesting stages on the seed quality of lentil (Lens culinaris L.) during storage. Bangladesh J Argil Res 34(4):565–576

    Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial support and facilities received from the Department of Agriculture, the Karnataka State Biofuel Development Board, the Government of Karnataka and the University of Agricultural Sciences, GKVK, Bengaluru and the Department of Applied Botany, School of Biosciences, Kuvempu University.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Balakrishna Gowda.

About this article

Cite this article

Pavithra, H.R., Gowda, B., Rajesh Kumar, K. et al. Oil, Fatty Acid Profile and Karanjin Content in Developing Pongamia pinnata (L.) Pierre Seeds. J Am Oil Chem Soc 89, 2237–2244 (2012). https://doi.org/10.1007/s11746-012-2126-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-012-2126-7

Keywords

Navigation