Journal of the American Oil Chemists' Society

, Volume 89, Issue 7, pp 1183–1191 | Cite as

A Dynamic Light Scattering Study on the Complex Assembly of Glycinin Soy Globulin in Aqueous Solutions

  • Víctor M. Pizones Ruiz-Henestrosa
  • María J. Martinez
  • Juan M. R. Patino
  • Ana M. R. Pilosof
Original Paper

Abstract

In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10−1% w/w and 20 °C, respectively, and the pH was 7.6, 7.0 and 3.0. By analyzing the intensity and volume size distributions, a complex equilibrium between self-assembled forms could be determined. At pH 7.6 and an ionic strength of 0.5 M, where the self-assembly of glycinin has been widely reported in the literature, the DLS technique revealed an equilibrium between different assembled forms, that shifted towards the 11S form. At a lower ionic strength for pH 3.0 or 7.0, the 7S form predominated. The hydrodynamic diameter evolved differently upon heating, depending on pH and ionic strength. For pH 7 (I = 0.05) and 7.6 (I = 0.5) a significant increase in dH was observed at a temperatures of 55 and 70 °C, respectively, which were significantly lower than the denaturation onset temperatures as determined by DSC. No changes in dH nor a transition endotherm were observed at pH 3.

Keywords

Globulin Soy Dynamic light scattering Structure 

Abbreviations

DLS

Dynamic light scattering

SBS

Sodium bisulfite

SDS

Sodium dodecyl sulfate

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Copyright information

© AOCS 2012

Authors and Affiliations

  • Víctor M. Pizones Ruiz-Henestrosa
    • 1
    • 2
  • María J. Martinez
    • 1
  • Juan M. R. Patino
    • 2
  • Ana M. R. Pilosof
    • 1
  1. 1.Departamento de Industrias, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.Departamento de Ingeniería Química, Facultad de QuímicaUniversidad de SevillaSevillaSpain

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