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Triacylglycerols Oxidation in Oils and Fats Monitored by Easy Ambient Sonic-Spray Ionization Mass Spectrometry

  • Original Paper
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Journal of the American Oil Chemists' Society

Abstract

Oxidation is the major cause of fat and oil deterioration which produces rancid and unpleasant flavors that drastically reduces their quality and nutritional value. The primary oxidation products are triacylglycerols (TAG) containing unsaturated fatty acyl hydroperoxides formed by a chain process occurring through free radical intermediates. The Rancimat method is currently the gold standard for measuring the oxidative stability of oils and fats. In this report, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is demonstrated to function as a direct (no pre-separation or sample preparation steps), fast and accurate method to monitor oils and fats oxidation, providing detailed overviews of the most immediate TAG hydroperoxide products. The EASI-MS results were compared to those obtained via the classical Rancimat test.

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Acknowledgments

We thank the State of São Paulo Research Foundation (FAPESP), the Brazilian National Council for Scientific and Technological Development (CNPq) and the Financing Agency of Studies and Projects (FINEP) for financial assistance. We also thank Dra Carmen Dobarganes for suggestions and revision of this manuscript.

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Authors and Affiliations

  1. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, UNICAMP, Campinas, SP, 13083-970, Brazil

    R. C. Simas, M. N. Eberlin & R. M. Alberici

  2. Fats and Oils Laboratory, Faculty of Food Engineering, University of Campinas, UNICAMP, Campinas, SP, 13083-970, Brazil

    D. Barrera-Arellano

  3. Department of Clinical Pathology, School of Medical Sciences, University of Campinas, UNICAMP, Campinas, SP, 13083-970, Brazil

    R. R. Catharino

  4. Division of Chemical Metrology, National Institute of Metrology, Quality and Technology, Duque de Caxias, RJ, 25250-020, Brazil

    V. Souza

Authors
  1. R. C. Simas
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  2. D. Barrera-Arellano
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  3. M. N. Eberlin
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  4. R. R. Catharino
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  5. V. Souza
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  6. R. M. Alberici
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Correspondence to R. M. Alberici.

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Simas, R.C., Barrera-Arellano, D., Eberlin, M.N. et al. Triacylglycerols Oxidation in Oils and Fats Monitored by Easy Ambient Sonic-Spray Ionization Mass Spectrometry. J Am Oil Chem Soc 89, 1193–1200 (2012). https://doi.org/10.1007/s11746-012-2010-5

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  • DOI: https://doi.org/10.1007/s11746-012-2010-5

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