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Estimation of Glycerides and Free Fatty Acid in Oils Extracted From Various Seeds from the Indian Region by NMR Spectroscopy

Abstract

The present study is focused on the quantification of glycerides and free fatty acid in oils extracted from various seeds for biodiesel production from Indian territory. A new method based on 1H- and 13C-NMR spectroscopic techniques was developed in order to estimate triglycerides (TG), diglycerides, monoglycerides, free fatty acids (FFA) and various other components such as para-substituted phenols, silylated methyl esters, aromatic acids, naphthalenes, etc. Iodine values of the extracted oils were estimated using the developed 1H-NMR spectroscopic method, which was correlated with the TG content present in the sample. Results by the NMR method were validated by the blend preparation, fatty acid composition determined by the GC and from the iodine value of the samples. The developed method is direct, rapid and no sample treatment is required. The results from these comprehensive studies indicated that NMR spectroscopic technique is useful for the quantification of extracted oils and can be used effectively for the development and monitoring of biodiesel production and determining the fuel quality.

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Acknowledgments

The authors wish to acknowledge the Management of IOC, R&D Faridabad for allowing them to publish the present work.

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Correspondence to Ravindra Kumar.

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Kumar, R., Bansal, V., Tiwari, A.K. et al. Estimation of Glycerides and Free Fatty Acid in Oils Extracted From Various Seeds from the Indian Region by NMR Spectroscopy. J Am Oil Chem Soc 88, 1675–1685 (2011). https://doi.org/10.1007/s11746-011-1846-4

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  • DOI: https://doi.org/10.1007/s11746-011-1846-4

Keywords

  • Vegetable oil
  • Biodiesel
  • Glycerides
  • 1H-NMR spectroscopy
  • 13C-NMR spectroscopy