Journal of the American Oil Chemists' Society

, Volume 88, Issue 11, pp 1767–1774 | Cite as

Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed (Cannabis sativa L.) Protein Hydrolysates

  • Abraham T. Girgih
  • Chibuike C. Udenigwe
  • Huan Li
  • Abayomi P. Adebiyi
  • Rotimi E. AlukoEmail author
Original Article


The aim of this study was to determine the antihypertensive effects of enzymatic hemp seed protein hydrolysate (HPH) and its peptide fractions. Hemp seed protein isolate was digested by the sequential action of pepsin and pancreatin to mimic gastrointestinal digestion in human beings. The resultant HPH was separated by membrane ultrafiltration into peptide fractions with different sizes (<1 and 1–3 kDa). The HPH led to significantly higher (P < 0.05) in vitro inhibition of the activities of angiotensin I-converting enzyme (ACE) and renin, the two main enzymes involved in abnormal blood pressure elevation (hypertension). Kinetic studies showed that HPH and peptide fractions inhibited renin and ACE activities in a mixed-type pattern, indicating binding to areas other than the active site. Oral administration of HPH (200 mg/kg body weight) to spontaneously hypertensive rats led to significant reductions (P < 0.05) in systolic blood pressure (SBP) that reached a maximum of −30 mmHg after 8 h. In contrast, the hypotensive effects of peptide fractions (<1 and 1–3 kDa) had a maximum value of about −15 mmHg after 6–8 h post oral administration. The results suggest a synergistic antihypertensive effect of the peptides present within HPH; this effect was reduced significantly (P < 0.05) upon separation into peptide fractions.


Enzyme inhibition kinetics Renin Angiotensin converting enzyme Antihypertensive properties Spontaneously hypertensive rats Hemp seed Protein hydrolysate IC50 



This work was funded through a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to R.E.A.


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Copyright information

© AOCS 2011

Authors and Affiliations

  • Abraham T. Girgih
    • 1
  • Chibuike C. Udenigwe
    • 1
  • Huan Li
    • 1
  • Abayomi P. Adebiyi
    • 1
    • 2
  • Rotimi E. Aluko
    • 1
    Email author
  1. 1.Department of Human Nutritional SciencesUniversity of ManitobaWinnipegCanada
  2. 2.The Richardson Centre for Functional Foods and NutraceuticalsUniversity of ManitobaWinnipegCanada

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