Abstract
Using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, collaborating scientists in ten different laboratories measured (in duplicate) the total trans fat content of ten fat or oil test samples, two of which were blind duplicates. The procedure used entailed measuring the height of the negative second derivative of the IR absorption band at 966 cm−1. This absorption is attributed to the C–H deformation vibration that is characteristic of isolated (non-conjugated) double bonds with the trans configuration. The precision of ATR-FTIR results in this international collaborative study was satisfactory and led to the approval of this validated procedure as official method AOCS Cd 14e-09 in late 2009. This official method is also suitable for analysis of total isolated trans fat and oil products containing, or supplemented with, trans conjugated linoleic acid (CLA) isomers. Although this method does not require derivatization of the oil or fat test materials, as required for GC, fats and oils in foods must be extracted with organic solvents before analysis. This method is also rapid (5 min) and does not require any weighing or quantitative dilution of unknown neat fat or oil test samples in any solvent. The AOCS Cd 14e-09 method is suitable for determination of test samples with zero trans fat, which is defined according to the US labeling regulations as 0.5 g trans fat per serving or 1.8% trans fat, as a percentage of total fat.
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Abbreviations
- FT:
-
Fourier transform
- IR:
-
Infrared
- ATR:
-
Attenuated total reflection
- PHCO:
-
Partially hydrogenated canola oil
- TE:
-
Trielaidin
- TP:
-
Tripalmitin
References
AOCS Official Method Cd14d-99 (1999) Rapid determination of isolated trans geometric isomers in fats and oils by attenuated total reflection infrared spectroscopy
AOAC Official Method 2000.10 (2000) Determination of total isolated trans unsaturated fatty acids in fats and oils. ATR-FTIR spectroscopy
Milosevic M, Milosevic V, Kramer JKG, Azizian H, Mossoba MM (2004) Determining low levels of trans fatty acids in foods by an improved ATR-FTIR procedure. Lipid Technol 16:252–264
Mossoba MM, Kramer JKG, Milosevic V, Milosevic M, Azizian H (2007) Interference of saturated fats in the determination of low levels of trans fats (below 5%) by infrared spectroscopy. J Am Oil Chem Soc 84:339–342
Mossoba MM, Milosevic V, Milosevic M, Kramer JKG, Azizian H (2007) Determination of total trans fats and oils by infrared spectroscopy for regulatory compliance. Anal Bioanal Chem 389:87–92 special issue on food and dietary supplements
Mossoba MM, Seiler A, Kramer JKG, Milosevic V, Milosevic M, Azizian H, Steinhart H (2009) Nutrition labeling: rapid determination of total trans fats by using internal reflection infrared spectroscopy and a second derivative procedure. J Am Oil Chem Soc 86:1037–1045
AOCS Official Method Ce1h-05 (2005) Determination of cis-, trans-, saturated, monounsaturated and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC
Mossoba MM, McDonald RE, Armstrong DJ, Page SW (1991) Identification of minor C18 triene and conjugated diene isomers in hydrogenated soybean oil and margarine by GC-MI-FTIR spectroscopy. J Chromatogr Sci 29:324–330
DHHS/FDA (1999) Food labeling: trans fatty acids in nutrition labeling, nutrient content claims, and health Claims, 64 Fed. Reg. 62746 (November 17, 1999)
Schrimpf-Moss J, Wilkening V (2005) Trans fat—new FDA regulations. In: Kodali DR, List GR (eds) Trans fat alternatives. AOCS Press, Champaign, pp 26–33
Satchithanandam S, Oles CJ, Spease CJ, Brandt MM, Yurawecz MP, Rader JI (2004) Trans, saturated, and unsaturated fat in foods in the United States prior to mandatory trans-fat labeling. Lipids 39:11–18
AOCS Official Method Cd 14e-09 (2009) Negative second derivative infrared spectroscopic method for the rapid (5 min) determination of total isolated trans fat, (online)
Anon (1995) Guidelines for collaborative study procedure to validate characteristics of a method of analysis. J Assoc Off Anal Chem 78(5):143A–160A
Kramer JKG, Hernandez M, Cruz-Hernandez C, Kraft J, Dugan MER (2008) Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2, 18:3 and CLA isomers of milk fat as demonstrated using Ag-ion SPE fractionation. Lipids 43:259–273
Horwitz W, Albert R (2006) The Horwitz ratio (HorRat): a useful index of method performance with respect to precision. J Assoc Off Anal Chem 89:1095–1109
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Mossoba, M.M., Seiler, A., Steinhart, H. et al. Regulatory Infrared Spectroscopic Method for the Rapid Determination of Total Isolated Trans Fat: A Collaborative Study. J Am Oil Chem Soc 88, 39–46 (2011). https://doi.org/10.1007/s11746-010-1648-0
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DOI: https://doi.org/10.1007/s11746-010-1648-0