Abstract
The aim of this study was to explore the use of glyceryl behenate (GB) as a candidate lipid for the production of solid lipid nanoparticles (SLN) using the anti-inflammatory drug, aceclofenac, as an example. SLN were produced using the Gasco microemulsion method with three different lipids, namely GB, glyceryl palmitostearate (GP) and cetyl alcohol (CA). The prepared SLN were subjected to determination of entrapment, zeta potential, particle size, in vitro dissolution, entrapment efficiency and biodistribution. Stable SLN of GB having size 245 ± 5 nm were prepared with a polydispersity index of 0.470. The size range was higher with other lipids i.e., CA and GP. It was found that as the drug lipid molar concentration was raised, particles with a smaller size were obtained irrespective of the nature of the lipid. The surfactant poloxamer 188 gave best results when used at a concentration of 2.5% w/v of dispersion. The study recommends GB as a suitable candidate for the production of SLN.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AC:
-
Aceclofenac
- CA:
-
Cetyl alcohol
- GB:
-
Glyceryl behenate
- GP:
-
Glyceryl palmitostearate
- SLN:
-
Solid lipid nanoparticles
- SR:
-
Sustained release
References
Manjunath K, Reddy SJ, Venkateshwarlu V (2005) Solid lipid nanoparticles as drug delivery systems. Methods Find Exp Clin Pharmacol 27:1–20
Utreja S, Jain NK (2001) Solid lipid nanoparticles. In: Jain NK (ed) Advances in controlled and novel drug delivery. CBS Publishers, New Delhi, pp 408–425
Mader K (2006) Solid lipid nanoparticles as drug carriers. In: Trochilin VP (ed) Nanoparticulates as drug carriers. Imperial College Press, London, pp 187–212
Müller RH, Mäder K, Gohla S (2000) Solid lipid nanoparticles (SLN) for controlled drug delivery—a review of the state of the art. Eur J Pharm Biopharm 50:161–177
Mehnert W, Mäder K (2001) Solid lipid nanoparticles production, characterization and applications. Adv Drug Deliv Rev 47:165–196
Akimoto H, Yamazaki R, Hashimoto S, Sato T, Ito A (2000) 4′-Hydroxy aceclofenac suppresses the interleukin-1 induced production of promatrix metalloproteinases and release of sulfated-glycosaminoglycans from rabbit articular chondrocytes. Eur J Pharmacol 401:429–436
Souto EB, Mehnert W, Muller RH (2006) Polymorphic behaviour of compritol® ATO 888 as bulk lipid and as SLN and NLC. J Microencapsul 23:417–433
Gasco MR (1993) Method for producing solid lipid microspheres having a narrow size distribution. US Patent 5,250,236
Roy RK (2001) Design of experiments using the Taguchi approach, 1st edn. Wiley, New York
Silverstein RM, Webster FX (2004) Spectrometric identification of organic compounds. Wiley, New York, pp 79–109
Pavia DL, Lampman GM, Kriz GS, Vyvyan JR (2007) Introduction to spectroscopy. 1st Indian ed. Brooks/Cole CENGAGE Learning, New Delhi, pp 26–107
Jose GR, Josephine D, Rios SA (2003) Controlled release pharmaceutical composition. US Patent 6,596,308
Acknowledgments
The authors wish to thank Babu Banarasi Das Group of Educational Institutions (BBDGEI), Lucknow, India for providing the necessary infrastructure and the National Institute of Pharmaceutical Education and Research (NIPER), Mohali, India for their facilities.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Chawla, V., Saraf, S.A. Glyceryl Behenate and Its Suitability for Production of Aceclofenac Solid Lipid Nanoparticles. J Am Oil Chem Soc 88, 119–126 (2011). https://doi.org/10.1007/s11746-010-1618-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11746-010-1618-6