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Comparative Evaluation of Vegetable Oils-Impregnated Layers as Reversed-Phases for Thin-layer Chromatography

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Journal of the American Oil Chemists' Society

Abstract

Silica gel plates impregnated with a variety of oils including vegetable oils (olive, sunflower and corn oil) and synthetic oils (trioctylamine and paraffin oil) were evaluated and compared with the commercially available reversed-phases TLC plates (RP-18, RP-18W, and CN). A series of selected lipophilic vitamins was employed to evaluate the suitability of oils as reversed-phases for TLC and to provide different lipophilicity indices: R M0, scores corresponding to the first principal component of R F and/or R M, the arithmetic mean of R F and R M values obtained with solvent mixture containing various concentrations of methanol in water. The retention results were excellent (r > 0.98) and allowed accurate estimation of lipophilicity of selected vitamins and to ranking the lipophilicity of oils when comparing with chemically bonded phases. Concerning the lipophilicity scale of vegetable oils, it is worth noting that corn oil presents the highest lipophilicity, closely followed by the olive and sunflower oils.

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References

  1. Gocan S, Cimpan G, Comer J (2005) Lipophilicity measurements by liquid chromatography. In: Grushka E, Grinberg N (eds) Advances in chromatography. Taylor and Francis, Oxford, pp 79–176

    Google Scholar 

  2. Rekker RF, Mannhold R (1992) Calculation of drug lipophilicity. VCH, Weinheim

    Google Scholar 

  3. Hansch H, Leo A (1995) Exploring QSAR: fundamentals and applications in chemistry and biology. ACS, Washington DC

    Google Scholar 

  4. Karelson M (2000) Molecular descriptors in QSAR/QSPR. Wiley, New York

    Google Scholar 

  5. Kaliszan R (1997) Structure and retention in chromatography: a chemometric approach. Harwood, Amsterdam

    Google Scholar 

  6. Kaliszan R (2007) QSRR: quantitative structure-(chromatographic) retention relationships. Chem Rev 107:3212–3246

    Article  CAS  Google Scholar 

  7. Giaginis C, Tsantili-Kakoulidou A (2008) Current state of the art in HPLC methodology for lipophilicity assessment of basic drugs: a review. J Liq Chromatogr Rel Technol 31:79–96

    Article  CAS  Google Scholar 

  8. Djaković-Sekulić TL, Perišić-Janjić NU, Petrović SD (2002) Normal- and reversed-phase chromatography of para-substituted propanoic acid amides. J Planar Chromatogr 15:274–279

    Article  Google Scholar 

  9. Kastner P, Klimeš J, Zimová G, Klimešová V (2001) Reversed-phase thin-layer chromatographic determination of the lipophilicity of potential antituberculotic compounds. J Planar Chromatogr 14:291–295

    Article  CAS  Google Scholar 

  10. Djaković-Sekulić TL, Sârbu C, Perišić-Janjić NU (2005) A comparative study of the lipophilicity of benzimidazole and benztriazole derivatives by RPTLC. J Planar Chromatogr 18:432–436

    Article  Google Scholar 

  11. Perišić-Janjić NU, Djaković-Sekulić TL (2006) Study of the characteristics and separation properties of unconventional TLC supports: part I. J Planar Chromatogr 19:438–442

    Article  Google Scholar 

  12. Pyka A, Babuśka M, Śliwiok J (2006) Use of liquid chromatography and theoretical computational methods to compare the lipophilicity of selected cortisone derivatives. J Planar Chromatogr 19:432–437

    Article  CAS  Google Scholar 

  13. Djaković-Sekulić TL, Perišić-Janjić NU (2007) Study of the characteristics and separating power of unconventional TLC supports, II: principal-components analysis. J Planar Chromatogr 20:7–11

    Article  Google Scholar 

  14. Csermely T, Petroianu G, Kuca K, Fûrész J, Darvas F, Gulyás Z, Laufer R, Kalász H (2007) TLC of quaternary pyridinium aldoximes, antidotes of organophosphorus esterase inhibitors. J Planar Chromatogr 20:39–42

    Article  CAS  Google Scholar 

  15. Kresta J, Kastner P, Klimeš J, Klimešová V (2005) Reversed-phase thin-layer chromatographic determination of the lipophilicity of potential antituberculotic compounds. J Planar Chromatogr 18:450–454

    Article  CAS  Google Scholar 

  16. Mrkvičková Z, Kovaříková P, Klimeš J, Doležal M (2006) Determination of the lipophilicity of potential antituberculotic compounds by RPTLC. J Planar Chromatogr 19:422–426

    Article  Google Scholar 

  17. Pyka A (2004) New method of calculation of the partition coefficients of selected alkoxyphenols investigated by RPTLC. J Planar Chromatogr 17:58–60

    Article  CAS  Google Scholar 

  18. Lascu D (2008) Food encyclopedia, All, Bucharest

  19. Bate-Smith EC, Westall RG (1950) Chromatographic behaviour and chemical structure, I: some naturally occurring phenolic substances. Biochim Biophys Acta 4:427–440

    Article  Google Scholar 

  20. Sârbu C, Tudor S (1998) Determination of lipophilicity of some non-steroidal anti-inflammatory agents and their relationships by using principal component analysis based on thin-layer chromatographic retention data. J Chromatogr A 822:263–269

    Article  Google Scholar 

  21. Sârbu C, Malawska B (2000) Evaluation of lipophilicity of piperazine derivatives by thin layer chromatography and principal component analysis. J Liq Chromatogr Rel Technol 23:2143–2154

    Article  Google Scholar 

  22. Sârbu C, Djaković-Sekulić TL, Perišić-Janjić NU (2002) Evaluation of lipophilicity of some benzimidazole and benztriazole derivatives by RP HPTLC and PCA. J Pharm Biomed Anal 30:739–745

    Article  Google Scholar 

  23. Sârbu C, Casoni D, Darabantu M, Maiereanu C (2004) Quantitative structure -retention and retention-activity relationships of some 1,3-oxazolidine systems by RP-HPTLC and PCA. J Pharm Biomed Anal 35:213–219

    Article  Google Scholar 

  24. Casoni D, Kot-Wasik A, Namiesnik J, Sârbu C (2009) Lipophilicity data for some preservatives estimated by reversed-phase liquid chromatography and different computation methods. J Chromatogr A 1216:2456–2465

    Article  CAS  Google Scholar 

  25. Sârbu C (1995) A comparative study of regression concerning weighted least squares methods. Anal Lett 28:2077–2094

    Google Scholar 

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Acknowledgments

The financial support of the Ministry of Education and Research of Romania (CNCSIS, IDEI 560/2007) is gratefully acknowledged.

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Authors and Affiliations

  1. Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, Arany Janos Str. No 11, 400028, Cluj Napoca, Romania

    Rodica Domnica Briciu & Costel Sârbu

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  1. Rodica Domnica Briciu
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  2. Costel Sârbu
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Correspondence to Costel Sârbu.

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Briciu, R.D., Sârbu, C. Comparative Evaluation of Vegetable Oils-Impregnated Layers as Reversed-Phases for Thin-layer Chromatography. J Am Oil Chem Soc 87, 1091–1102 (2010). https://doi.org/10.1007/s11746-010-1590-1

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  • DOI: https://doi.org/10.1007/s11746-010-1590-1

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