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Optimization of an Oil Extraction Process for Algae from the Treatment of Manure Effluent

Journal of the American Oil Chemists' Society volume 86, pages 909–915 (2009)Cite this article

Abstract

Increasing interest in the coupling of biological wastewater treatment processes with the generation of value-added products (such as oil containing ω-3 fatty acids (FA)) has stimulated efforts in adapting extraction methods for treatment byproducts. This study’s objective was to compare a high temperature/pressure extraction method (accelerated solvent extraction) (ASE) and a manual extraction method (modified Folch extraction) with regard to their ability to extract total oil from three algae samples from the treatment of dairy manure effluent. The efficiency of total oil and FA extraction with three solvents (chloroform/methanol, isopropanol/hexane, and hexane) was also evaluated using the ASE method. Results showed that the ASE method yielded higher values for total oil content compared to the Folch method but similar values for FA content and composition after four extraction cycles with chloroform/methanol. However, the ASE method yielded much higher amounts of FA in the first cycle (85–95% of total extracted) compared to the Folch method (44–55% of total extracted in the first cycle). As expected, the extraction efficiency of the ASE method for FA was dependent on the extraction solvent. FA content values using ASE with chloroform/methanol > isopropanol/hexane > hexane. FA content values using the Folch method or ASE with chloroform/methanol were not significantly influenced by sample particle size within the size range of 0.1–1 mm.

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References

  1. Cantrell KB, Ducey T, Ro KS, Hunt PG (2008) Livestock waste-to-bioenergy generation opportunities. Bioresour Technol 99:7941–7953

    Article  CAS  Google Scholar 

  2. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306

    Article  CAS  Google Scholar 

  3. Farrell AE, Gopal AR (2008) Bioenergy research needs for heat, electricity, and liquid fuels. MRS Bull 33:373–387

    CAS  Google Scholar 

  4. Hu Q, Sommerfield M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54:621–639

    Article  CAS  Google Scholar 

  5. Mulbry W, Kondrad S, Buyer J (2008) Treatment of dairy and swine manure effluents using freshwater algae: fatty acid content and composition of algal biomass at different manure loading rates. J App Phycol 20:1079–1085

    Article  Google Scholar 

  6. Benemann JR, Oswald WJ (1996) Systems and economic analysis of microalgae ponds for conversion of carbon dioxide to biomass. Pittsburgh Energy Technology Center, Pittsburgh. Available via http://govdocs.aquake.org/cgi/reprint/2004/915/9150050 Cited 14 Oct 2008

  7. Aresta M, Dibenedetto A, Barberio G (2005) Utilization of macro-algae for enhanced CO2 fixation and biofuels production: development of computing software for an LCA study. Fuel Process Technol 86:1679–1693

    Article  CAS  Google Scholar 

  8. Chesapeake Bay Commission, Commonwealth of Pennsylvania (2008) Next generation biofuels: taking the policy lead for the nation. Available via http://www.chesbay.state.va.us/Publications/nexgen%20biofuels1.pdf Cited 14 Oct 2008

  9. Groom MJ, Gray EM, Townsend PA (2008) Biofuels and biodiversity: principles for creating better policies for biofuel production. Conserv Biol 22:602–609

    Article  Google Scholar 

  10. Vasudevan PT, Briggs M (2008) Biodiesel production: current state of the art and challenges. J Ind Microbiol Biotechnol 35:421–430

    Article  CAS  Google Scholar 

  11. Wilkie AC, Mulbry WW (2002) Recovery of dairy manure nutrients by benthic freshwater algae. Bioresour Technol 84:81–91

    Article  CAS  Google Scholar 

  12. Pizarro C, Mulbry W, Blersch M, Kangas P (2006) An economic assessment of algal turf scrubber technology for treatment of dairy manure effluent. Ecol Engin 26:321–327

    Article  Google Scholar 

  13. Franklin ST, Martin KR, Baer RJ, Schingoethe DJ, Hippen AR (1999) Dietary marine algae (Schizochytrium sp.) increases concentrations of conjugated linoleic, docosahexaenoic, and trans-vaccenic acids in milk of dairy cows. J Nutrit 129:2048–2054

    CAS  Google Scholar 

  14. Mulbry W, Kondrad S, Pizarro C (2008) Treatment of dairy manure effluent using freshwater algae: algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. Bioresour Technol 99:8137–8142

    Article  CAS  Google Scholar 

  15. Folch J, Lees M (1957) A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  Google Scholar 

  16. Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can J Biochem Physiol 67:911–917

    Google Scholar 

  17. Cheung PK (1999) Temperature and pressure effects on supercritical carbon dioxide extraction of n-3 fatty acids from red seaweed. Food Chem 65:399–403

    Article  CAS  Google Scholar 

  18. Mendes RL, Reis AD, Palavra AF (2006) Supercritical CO2 extraction of linolenic acid and other lipids from Arthrospira (Spirulina) maxima: comparison with organic solvent extraction. Food Chem 99:57–63

    Article  CAS  Google Scholar 

  19. Schäfer K (1998) Accelerated solvent extraction of lipids for determining the fatty acid composition of biological material. Anal Chim Acta 358:69–77

    Article  Google Scholar 

  20. Luthria D, Vinjamoori D, Noel K, Ezzell J (2004) Accelerated solvent extraction. In: Luthria D (ed) Oil extraction and analysis: critical issues and comparative studies. AOCS Press, Champaign, IL, pp 25–38

    Google Scholar 

  21. Dionex (2004) Methods optimization in accelerated solvent extraction (ASE). technical note 208 available via http://www1.dionex.com/en-us/webdocs/4736_TN208.pdf Cited 14 Oct 2008

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Authors and Affiliations

  1. US Department of Agriculture, Beltsville Agricultural Research Center, Building 306, Room 109, BARC-East, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

    Walter Mulbry, Shannon Kondrad, Jeffrey Buyer & Devanand L. Luthria

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  1. Walter Mulbry
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  2. Shannon Kondrad
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  3. Jeffrey Buyer
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  4. Devanand L. Luthria
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Correspondence to Walter Mulbry.

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Mulbry, W., Kondrad, S., Buyer, J. et al. Optimization of an Oil Extraction Process for Algae from the Treatment of Manure Effluent. J Am Oil Chem Soc 86, 909–915 (2009). https://doi.org/10.1007/s11746-009-1432-1

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  • DOI: https://doi.org/10.1007/s11746-009-1432-1

Keywords

  • Accelerated solvent extraction
  • Algae
  • Manure
  • Bioenergy
  • Wastewater
  • Treatment