Skip to main content
SpringerLink
Log in

Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80–100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30–60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Ferrer M, Soliveri J, Ploua FJ, Cortés NL, Duarte DR, Christensen M, Patiño JLC, Ballesteros A (2005) Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties. Enzyme Microb Technol 36:391–398

    Article  CAS  Google Scholar 

  2. Šabeder S, Habulin M, Knez Ź (2006) Lipase-catalyzed synthesis of fatty acid fructose esters. J Food Eng 77:880–886

    Article  CAS  Google Scholar 

  3. Jia H, Wang P (2007) Enzymatic synthesis of galactosyl lactic ethyl ester and its polymer for use as biomaterials. J Biotechnol 132:314–317

    Article  CAS  Google Scholar 

  4. Wehtje E, Adlercreutz P (1997) Water activity and substrate concentration effects on lipase activity. Biotechnol Bioeng 55:798–806

    Article  CAS  Google Scholar 

  5. Matsumoto M, Odachi D, Kondo K (2001) Effect of water activity on rate of esterification by lipase in organic media. J Chem Eng Jpn 34:437–440

    Article  CAS  Google Scholar 

  6. Kawamura Y, Nakanishi K, Matsuno R (1981) Stability of immobilized α-chymotrypsin. Biotechnol Bioeng 23:1219–1236

    Article  CAS  Google Scholar 

  7. Yoshida Y, Kimura Y, Adachi S (2006) Thermal inactivation of immobilized lipase in 1-alcohols. J Biosci Bioeng 102:66–68

    Article  CAS  Google Scholar 

  8. Back JF, Oakenfull D, Smith MB (1979) Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 18:5191–5196

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by a Center-of-Excellence (COE) project for Microbial-Process Development Pioneering Future Production Systems, and was also supported as a Food Nanotechnology Project by the Ministry of Agriculture, Forestry, and Fisheries, Japan.

Author information

Authors and Affiliations

  1. Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Takashi Kobayashi, Takemasa Matsuo, Yukitaka Kimura & Shuji Adachi

Authors
  1. Takashi Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takemasa Matsuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yukitaka Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shuji Adachi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shuji Adachi.

About this article

Cite this article

Kobayashi, T., Matsuo, T., Kimura, Y. et al. Thermal Stability of Immobilized Lipase from Candida antarctica in Glycerols with Various Water Contents at Elevated Temperatures. J Am Oil Chem Soc 85, 1041–1044 (2008). https://doi.org/10.1007/s11746-008-1285-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-008-1285-z

Keywords

Search

Navigation