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Efficient Immobilization of Lecitase in Gelatin Hydrogel and Degumming of Rice Bran Oil Using a Spinning Basket Reactor

Journal of the American Oil Chemists' Society volume 85, pages 739–748 (2008)Cite this article

Abstract

Immobilization of Lecitase (Phospholipase A1) in gelatin hydrogel and its stability is studied with a view to utilizing the immobilized enzyme for degumming rice bran oil. Excellent retention of enzyme activity (>80%) is observed in hydrogel containing 43.5% gelatin crosslinked with glutaraldehyde. Compared to the free enzyme which has a broad pH-activity profile (6.5–8.0), the activity of the immobilized enzyme is strongly dependent on pH and has a pH-optimum of pH 7.5. The optimum temperature of enzyme activity increases from 37 to 50 °C. Compared to the free enzyme which loses all its activity in 72 h at 50 °C, the immobilized enzyme retains its activity in full. The immobilized enzyme has been used efficiently in a spinning basket bioreactor for the degumming of rice bran oil with 6 recycles without loss of enzyme activity. The phosphorus content of the oil decreases from 400 ppm to 50–70 ppm in each cycle. After charcoal treatment and dewaxing, a second enzymatic treatment brings down the phosphorus content to <5 ppm.

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Acknowledgments

We thank Dr. R. B. N. Prasad and Dr P. P. Chakrabarti, Lipid Science and Technology Division, IICT, Hyderabad, for helpful discussions; and CSIR, New Delhi, for financial support.

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Authors and Affiliations

  1. Biotransformations Laboratory, Indian Institute of Chemical Technology, Uppal Road, Habsiguda, Hyderabad, 500007, India

    Gurrala Sheelu, G. Kavitha & Nitin W. Fadnavis

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  1. Gurrala Sheelu
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  2. G. Kavitha
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  3. Nitin W. Fadnavis
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Correspondence to Nitin W. Fadnavis.

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Sheelu, G., Kavitha, G. & Fadnavis, N.W. Efficient Immobilization of Lecitase in Gelatin Hydrogel and Degumming of Rice Bran Oil Using a Spinning Basket Reactor. J Am Oil Chem Soc 85, 739–748 (2008). https://doi.org/10.1007/s11746-008-1261-7

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  • DOI: https://doi.org/10.1007/s11746-008-1261-7

Keywords

  • Phospholipase A1
  • Immobilization
  • Gelatin
  • Rice bran oil
  • Degumming
  • Spinning basket reactor