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Efficient Immobilization of Lecitase in Gelatin Hydrogel and Degumming of Rice Bran Oil Using a Spinning Basket Reactor


Immobilization of Lecitase (Phospholipase A1) in gelatin hydrogel and its stability is studied with a view to utilizing the immobilized enzyme for degumming rice bran oil. Excellent retention of enzyme activity (>80%) is observed in hydrogel containing 43.5% gelatin crosslinked with glutaraldehyde. Compared to the free enzyme which has a broad pH-activity profile (6.5–8.0), the activity of the immobilized enzyme is strongly dependent on pH and has a pH-optimum of pH 7.5. The optimum temperature of enzyme activity increases from 37 to 50 °C. Compared to the free enzyme which loses all its activity in 72 h at 50 °C, the immobilized enzyme retains its activity in full. The immobilized enzyme has been used efficiently in a spinning basket bioreactor for the degumming of rice bran oil with 6 recycles without loss of enzyme activity. The phosphorus content of the oil decreases from 400 ppm to 50–70 ppm in each cycle. After charcoal treatment and dewaxing, a second enzymatic treatment brings down the phosphorus content to <5 ppm.

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We thank Dr. R. B. N. Prasad and Dr P. P. Chakrabarti, Lipid Science and Technology Division, IICT, Hyderabad, for helpful discussions; and CSIR, New Delhi, for financial support.

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Correspondence to Nitin W. Fadnavis.

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Sheelu, G., Kavitha, G. & Fadnavis, N.W. Efficient Immobilization of Lecitase in Gelatin Hydrogel and Degumming of Rice Bran Oil Using a Spinning Basket Reactor. J Am Oil Chem Soc 85, 739–748 (2008).

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  • Phospholipase A1
  • Immobilization
  • Gelatin
  • Rice bran oil
  • Degumming
  • Spinning basket reactor