Journal of the American Oil Chemists' Society

, Volume 82, Issue 8, pp 565–569 | Cite as

Extraction of egg-yolk lecithin



In this research, the extraction of egg-yolk lecithin with ethanol was studied. Extraction was performed with deoiled and undeoiled yolks and with heated and unheated yolks. The yield of the extracted fraction relative to the initial material, phospholipid (PL) purity, and cholesterol content of both the PC-enriched fractions and the remaining PL fractions were determined. The yield and PL purity of the PC-enriched fractions obtained from the undeoiled yolks were 23.9 and 35.7%, and those obtained from deoiled yolks were 13.5 and 53.3%. The recovery of total PL in the two fractions was higher from the undeoiled (70%) than from the deoiled yolks (60%). However, heating had a negligible effect on PL extraction. Better enrichment of PC was observed by extraction from the undeoiled than from the deoiled yolks. The cholesterol content of the PC-enriched fraction obtained from the undeoiled yolks was much higher than that from the deoiled yolks.

Key Words

Cholesterol egg yolks extraction lecithin phospholipids 


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  1. 1.
    American Egg Board, Egg Products Reference Guide, (accessed November 15, 2002).Google Scholar
  2. 2.
    Chi, Y., and S. Lin, Research Advances in Extraction and Application of Egg-Yolk Lecithin, Food Ferment. Ind. 28:50–53 (2002).Google Scholar
  3. 3.
    Sim, J.S., New Extraction and Fractionation Method for Lecithin and Neutral Oil from Egg Yolk, in Egg Uses and Processing Technologies, edited by J.S. Sim and S. Nakai, CAB International, Wallingford, United Kingdom, 1994, pp. 128–138.Google Scholar
  4. 4.
    Jiang, Y., S.K. Noh, and S.I. Koo, Egg Phosphatidylcholine Decrease the Lymphatic Absorption of Cholesterol in Rats, J. Nutr. 131:2358–2363 (2001).Google Scholar
  5. 5.
    Sugino, H., M. Ishikawa, T. Nitoda, M. Koketsu, L.R. Juneja, M. Kim, and T. Yamamoto, Antioxidative Activity of Egg Yolk Phospholipids, J. Agric. Food Chem. 45:551–554 (1997).CrossRefGoogle Scholar
  6. 6.
    Larsen, J.E., and G.W. Froning, Extraction and Processing of Various Components from Egg Yolk, Poultry Sci. 60:160–167 (1981).Google Scholar
  7. 7.
    Tokarska, B., and M.T. Clandinin, Extraction of Egg Yolk Oil of Reduced Cholesterol Content, Can. Inst. Food Sci. Technol. J. 18:256–258 (1985).Google Scholar
  8. 8.
    Sim, J.S., Extraction of Fresh Liquid Egg Yolk, Canadian Patent 1,335,054 (1995).Google Scholar
  9. 9.
    Miyata, K., and H. Matsumoto, Manufacture of Purified Egg-Yolk Lecithins, Japanese Patent 2,001,072,693 (2001).Google Scholar
  10. 10.
    Nielsen, H., In situ Phase Extraction of Phospholipids from Heat-Coagulated Egg Yolk by Organic Solvents, Lebensm.-Wiss. Technol. 34:526–532 (2001).CrossRefGoogle Scholar
  11. 11.
    Nielsen, H., In situ Solid Phase Extraction of Lipids from Spray-Dried Egg Yolk by Ethanol with Subsequent Removal of Triacylglycerols by Cold Temperature Crystallization, 67:613–618 (2004).CrossRefGoogle Scholar
  12. 12.
    Hruschka, S.M., S. Kirchner, J. Rassenhovel, and W. Witt., Method for the Fractionation of Oil and Polar Lipid-Containing Native Raw Materials, U.S. Patent Application 20,030,054,084 (2003).Google Scholar
  13. 13.
    Wu, Y., and T. Wang, Soybean Lecithin Fractionation and Functionality, J. Am. Oil Chem. Soc. 80:319–326 (2003).Google Scholar
  14. 14.
    Warren, M.W., H.G. Brown, and D.R. Davis, Solvent Extraction of Lipid Components from Egg Yolk Solids, 65:1136–1139 (1988).Google Scholar
  15. 15.
    AOCS, Official Methods and Recommended Practices of the American Oil Chemists' Society, 4th edn., AOCS Press, Champaign, IL, 1994.Google Scholar
  16. 16.
    Folch, J., M. Lees, and G.H. Sloane Stanley, A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226:497–509 (1957).Google Scholar
  17. 17.
    Statistical Analysis System, SAS Institute, Cary, NC, 1999–2001.Google Scholar
  18. 18.
    Schneider, M., Fractionation and Purification of Lecithin, in Lecithins: Sources, Manufacture & Uses, edited by B.F. Szuhaj, AOCS Press, Champaign, IL, 1989, pp. 109–130.Google Scholar
  19. 19.
    Fatemi, S.H., and E.G. Hammond, Analysis of Oleate, Linolenate and Linolenate Hydroperoxides in Oxidized Ester Mixtures, Lipids 15:379–385 (1980).CrossRefGoogle Scholar
  20. 20.
    Wang, T., E.G. Hammond, and W.R. Fehr, Phospholipid Fatty Acid Composition and Stereospecific Distribution of Soybeans with a Wide Range of Fatty Acid Composition, J. Am. Oil Chem. Soc. 74:1587–1594 (1997).Google Scholar

Copyright information

© AOCS Press 2005

Authors and Affiliations

  1. 1.Department of Food Science and Human Nutrition, Center for Crops Utilization ResearchIowa State UniversityAmes.

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