Abstract
To clarify the effects of microwave roasting on the distribution of tocopherols and FA of phospholipids within soybeans, whole soybeans (Glycine max) were treated by microwave and further evaluted as compared to a raw sample. Tocopherol homologs, measured using HPLC, and phospholipid profiles, quantified with GC, were determined in the seed coat, the embryonic axis, and selections of cotyledons separated from three cultivars. The tocopherols were predominantly detected in the axis, followed by the cotyledons, and then very little in the coat. As much as 25% of the individual tocopherols originally present in the coat were lost at 12 min of roasting, whereas <25% was lost in the cotyledons and the axis after 20 min of roasting. The greatest rate of phospholipid loss (P<0.05) was observed in PE, followed by PC and PI, and their changing patterns were more pronounced in the coat than in the cotyledons or the axis. Thus, tocopherol content and phospholipid profiles change with microwave roasting according to tissue.
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Bau, H.M., C. Villaume, J.P. Nicolas, and L. Méjean, Effect of Germination on Chemical Composition, Biochemical Constitutents and Antinutritional Factors of Soya Bean (Glycine max) Seeds, J. Sci. Food Agric. 73:1–9 (1997).
Johnson, L.A., C.W. Deyoe, W.J. Hoover, and J.R. Schwenke, Inactivation of Trypsin Inhibitors in Aqueous Soybean Extracts by Direct Steam Infusion, Cereal Chem. 57:376–379 (1980).
Valle, F.R., Nutritional Qualities of Soya Protein as Affected by Processing, J. Am. Oil Chem. Soc. 58:419–429 (1981).
Hutton, K., and P.D. Foxcroft, Effect of Processing Temperature on Some Indices of Nutritional Significance for Micronized Soya Beans, Proc. Nutri Soc. 34:49A-50A (1975).
Decareau, R.V., Microwave Food Processing Equipment Throughout the World, J. Food Technol. 40:99–105 (1986).
Burfoot, D., S.J. James, A.M. Foster, K.P. Self, T.J. Wilkins, and I. Philips, Temperature Uniformity After Reheating in Microwave Ovens, in Processing Engineering in the Food Industry, edited by R.W. Field and J.A. Howell, Elsevier Applied Science, New York, 1990, Vol. 2, pp. 1–14.
Yoshida, H., and G. Kajimoto, Effects of Microwave Treatment on the Trypsin Inhibitor and Molecular Species of Triglycerides in Soybeans, J. Food Sci. 53:1756–1760 (1988).
Giese, J.H., Special Report: Advances in Microwave Food Processing, Food Technol. 46:118–123 (1992).
Morrison, W.R., and L.M. Smith, Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Fluoride-Methanol, J. Lipid Res. 5:600–608 (1964).
AOAC Official Methods of Analysis of AOAC International, 16th edn., edited by P. Cunniff, AOAC International, Gaithersburg, MD, 1995.
Yoshida, H., and S. Takagi, Microwave Roasting and Positional Distribution of Fatty Acids of Phospholipids in Soybeans (Glycine max L.), J. Am. Oil Chem. Soc. 74:915–921 (1997).
Folch, J., M. Lees, and G.H. Sloane-Stanley, A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226:497–509 (1957).
Yoshida, H., Composition and Quality Characteristics of Sesame Seed (Sesamum indicum) Oil Roasted at Different Temperatures in Electric Oven, J. Sci. Food Agric. 65:331–336 (1994).
Fritsch, C.W., Measurements of Frying Fat Deterioration. A Brief Review, J. Am. Oil Chem. Soc. 58:272–274 (1981).
Yoshida, H., S. Takagi, and S. Mitsuhashi, Tocopherol Distribution and Oxidative Stability of Oils Prepared from the Hypocotyl of Soybeans Roasted in a Microwave Oven, Ibid. 76:915–920 (1999).
Yoshida, H., and S. Takagi, Vitamin E and Oxidative Stability of Soya Bean Oil Prepared with Beans at Various Moisture Contents Roasted in a Microwave Oven, J. Sci. Food Agric. 72:111–119 (1996).
Yoshida, H., Y. Hirakawa, Y. Mizushina, and T. Tanaka, Molecular Species of Triacylglycerols in the Hulls of Sunflower Seeds (Helianthus annuus L.) Following Microwave Treatment, Eur. J. Lipid Sci. Technol. 104:347–352 (2002).
Steel, R.C., J.H. Torrie, and D.A. Dickey Principles and Procedures of Statistics: A Biometrical Approach, McGraw-Hill, Singapore, 1997.
SAS, SAS User's Guide: Statistical (Version 6), SAS Institute Inc., Cary, NC, 1989.
Bauernfeind, J.C., Tocopherols in Foods, in Vitamin E, a Comprehensive Treatise, edited by L.J. Machlin, Marcel Dekker, New York, 1980, pp. 99–169.
Collins, J.L., and B.F. Beaty, Heat Inactivation of Trypsin Inhibitor in Fresh Green Soybeans and Physiological Responses of Rats Fed the Beans, J. Food Sci. 45:542–546 (1980).
Liu, K., and E.A. Brown, Fatty Acid Compositions in Newly Differentiated Tissues of Soybean Seedlings, J. Agric. Food Chem. 44:1395–1398 (1996).
Takagi, S., H. Ienaga, C. Tsuchiya, and H. Yoshida, Microwave Roasting Effects on the Composition of Tocopherols and Acyl Lipids Within Each Structural Part and Section of a Soya Bean, J. Sci. Food Agric. 79:1155–1162 (1999).
Simpson, T.D., and L.K. Nakamura, Phospholipid Degradation in Membranes of Isolated Soybean Lipids Bodies, J. Am. Oil Chem. Soc. 66:1093–1096 (1989).
Farmer, L.J., and D.S. Mottram, Lipid-Maillard Interactions in the Formation of Volatile Aroma Compounds, in Trends in Flavour Research, edited by H. Maarse and D.C. van der Heji, Elsevier, Amsterdam, 1994, pp. 313–326.
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Yoshida, H., Matsuda, K., Hirakawa, Y. et al. Roasting effects on the distribution of tocopherols and phospholipids within each structural part and section of soybeans. J Amer Oil Chem Soc 80, 665–674 (2003). https://doi.org/10.1007/s11746-003-0756-3
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DOI: https://doi.org/10.1007/s11746-003-0756-3