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Tocopherols in sea buckthorn (Hippophaë rhamnoides L.) berry oil

Abstract

The free tocopherol content in whole berries of six sea buckthorn cultivars grown in northeastern Poland and Belorussia was determined with HPLC. The total free tocopherol content in oil from whole berries was 101.4–128.3 mg/100 g of oil. α-Tocopherol was the predominant tocopherol of sea buckthorn berries, and only traces of γ-tocopherol were detected in the oil. α- and δ-Tocopherols constituted 62.5–67.9% and 32.1–37.5% of total tocopherol, respectively. The total free tocopherol content in oil of sea buckthorn cv. Nadbaltycka increased during maturation from 40.4 to 109.8 mg/100 g of oil. Green berries contained a marked amount of γ-tocopherol, but its content rapidly declined to traces when the color of berries turned from green to olive-yellow.

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References

  1. Roussi, A., The Genus Hippophae L.: A Taxonomic Study, Ann. Bot. Fennici 8:177–227 (1971).

    Google Scholar 

  2. Beveridge, T., T.S.C. Li, B.D. Oomah, and A. Smith, Sea Buckthorn Products: Manufacture and Composition, J. Agric. Food Chem. 47:3480–3488 (1999).

    CAS  Article  Google Scholar 

  3. Gao, X., M. Ohlander, N. Jeppsson, L. Bjork, and V. Trajkovski, Changes in Antioxidant Effects and Their Relationship to Phytonutrients in Fruits of Sea Buckthorn (Hippophae rhamnoides L.) During Maturation, J. Agric. Food Chem. 48:1485–1490 (2000).

    CAS  Article  Google Scholar 

  4. Zhang, W., J. Yang, J. Duo, B. Ren, and J. Guo, Preliminary Study of Biochemical Constitutions of Berry of Sea Buckthorn Growing in Shanxi Province and Their Changing Trend, in Proceedings of International Symposium on Sea Buckthorn (H. rhamnoides L.), Xian, China, October 19–23, 1989, pp. 129–132.

  5. Yang, B., and H.P. Kallio, Fatty Acids Composition of Lipids in Sea Buckthorn (Hippophae rhamnoides L.) Berries of Different Origins, J. Agric. Food Chem. 49:1939–1947 (2001).

    CAS  Article  Google Scholar 

  6. Zadernowski, R., H. Nowak-Polakowska, B. Lossow, and J. Nesterowicz, Sea Buckthorn Lipids, J. Food Lipids 4:167–172 (1997).

    Google Scholar 

  7. Li, T.S.G., and L.C.H. Wang, Physiological Components and Health Effects of Ginseng, Echinacea, and Sea Buckthorn, in Functional Foods, Biochemical and Processing Aspects, edited by G. Mazza, Technomic, Lancaster, PA, 1998, pp. 329–356.

    Google Scholar 

  8. Yang, B., R.M. Karlsson, P.H. Oksman, and H.P. Kallio, Phytosterols in Sea Buckthorn (Hippophae rhamnoides L.) Berries: Identification and Effects of Different Origins and Harvesting Time, J. Agric. Food Chem. 49:5620–5629 (2001).

    CAS  Article  Google Scholar 

  9. Ma, M., C. Cui, and G. Feng, Studies on the Fruit Character and Biochemical Compositions of Some Forms of Chinese Sea Buckthorn (Hippophae rhamnoides subsp. sinensis) in Shanxi, China, in Proceedings of International Symposium on Sea Buckthorn (H. rhamnoides L.), Xian, China, October 19–23, 1989, pp. 19–23.

  10. Fu, Q., Q. Yang, and G. Yang, Analysis of α-Tocopherol Contents in Sea-Buckthorn Oil by Reversed Phase-High Performance Liquid Chromatography, J. Xi’an Med. Univ. 14:181–183 (1993).

    CAS  Google Scholar 

  11. Kallio, H., B. Yang, P. Peippo, R. Tahvonen, and R. Pan, Triacylglycerols, Glycerophospholipids, Tocopherols, and Tocotrienols in Berries and Seeds of Two Subspecies (ssp. sinensis and mongolica) of Sea Buckthorn (Hippophaë rhamnoides), J. Agric. Food Chem. 50:3004–3009 (2002).

    CAS  Article  Google Scholar 

  12. Folch, J., A.M. Lees, J.A. Meath, and F.N. LeBaron, Preparation of Lipid Extracts from Brain Tissue, J. Biol. Chem. 191: 833–841 (1951).

    CAS  Google Scholar 

  13. Thompson, J.N., and G. Hatine, Determination of Tocopherols and Tocotrienols in Foods and Tissues by High-Performance Liquid Chromatography, J. Liquid Chromatogr. 2:327–344 (1979).

    CAS  Google Scholar 

  14. Eittenmiller, R.R., W.O. Landen, Jr., and J. Augustin, Vitamin Analysis, in Food Analysis, edited by S.S. Nielsen, Aspen, Gaithersburg, MD, 1998, pp. 281–291.

    Google Scholar 

  15. Xu, Z., Analysis of Tocopherols and Tocotrienols, in Current Protocols in Food Analytical Chemistry, edited by R. Wrolstadt, T.E. Acree, H. An, E.A. Decker, M.H. Penner, D.S. Reid, S.J. Schwartz, C.F. Shoemaker, and P. Sporns, John Wiley & Sons, New York, 2000.

    Google Scholar 

  16. McLaughlin, P.J., and J.L. Weihrauch, Vitamin E Content of Foods, J. Am. Diet. Assoc. 75:647–665 (1979).

    CAS  Google Scholar 

  17. Oomah, B.D., E.O. Kenaschuk, and G. Mazza, Tocopherols in Flaxseed, J. Agric. Food Chem. 45:2076–2080 (1997).

    CAS  Article  Google Scholar 

  18. Psomiadou, E., M. Tsimidou, and D. Boskou, α-Tocopherol Content of Greek Virgin Olive Oils, J. Agric. Food Chem. 48: 1170–1775 (2000).

    Article  Google Scholar 

  19. Demurin, Y., Genetic Variability in Tocopherol Composition in Sunflower Seeds, Helia 16:59–62 (1993).

    Google Scholar 

  20. Hashim, I.B., P.E. Koehler, and R.R. Eitenmiller, Tocopherols in Runner and Virginia Peanut Cultivars at Various Maturity Stages, J. Am. Oil Chem. Soc. 70:633–635 (1993).

    CAS  Google Scholar 

  21. Grams, G.W., and K. Eskins, Dye-Sensitized Photo-oxidation of Tocopherols: Correlation Between Singlet Oxygen Reactivity and Vitamin E Activity, Biochemistry 11:606–608 (1972).

    CAS  Article  Google Scholar 

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Correspondence to M. Naczk.

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Zadernowski, R., Naczk, M. & Amarowicz, R. Tocopherols in sea buckthorn (Hippophaë rhamnoides L.) berry oil. J Amer Oil Chem Soc 80, 55–58 (2003). https://doi.org/10.1007/s11746-003-0650-z

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  • DOI: https://doi.org/10.1007/s11746-003-0650-z

Key Words

  • Berries
  • HPLC
  • oil
  • sea buckthorn
  • tocopherols