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Journal of the American Oil Chemists' Society

, Volume 79, Issue 8, pp 749–753 | Cite as

Characterization of the TAG of peanut oil by electrospray LC-MS-MS

  • Craig A. DorschelEmail author
Article

Abstract

A study of processed peanut oil was undertaken to assess the utility of HPLC combined with tandem MS to obtain data easily regarding the number of TAG of fats and oils and their FA composition. Mass chromatograms and spectra corresponding to only TAG of a single M.W. were obtained for the full range of TAG in the sample. Analysis of the mass spectra allowed the identification of more than 160 TAG in the sample by their FA composition. In addition, it was possible to estimate relative abundances of the TAG and suggest the position of the FA on glycerol for a limited number of cases. This technique greatly simplifies the task of assigning FA to coeluting TAG and facilitates identification of TAG present in trace quantities in mixtures, with possible application in circumstances where such trace TAG could be significant markers. Results are quickly obtained without extensive sample preparation or prefractionation of the sample.

Key Words

Chromatography HPLC LC-MS LC-MS-MS mass spectrometry MS-MS oils peanut oil triacylglycerols triglycerides 

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References

  1. 1.
    Marini, D., HPLC of Lipids, in Food Analysis by HPLC, edited by L.M.L. Nollet, Marcel Dekker, New York, 1992, pp. 169–240, and references cited therein.Google Scholar
  2. 2.
    Byrdwell, W.C., and W.E. Neff, Qualitative and Quantitative Analysis of Triacylglycerols Using Atmospheric-Pressure Chemical Ionization Mass Spectrometry, in New Techniques and Applications in Lipid Analysis, edited by R.E. McDonald and M.M. Mossoba, AOCS Press, Champaign, 1997, pp. 45–80 and references cited therein.Google Scholar
  3. 3.
    Duffin, K.L., J.D. Henion, and J.J. Shieh, Electrospray and Tandem Mass Spectrometric Characterization of Acylglycerol Mixtures That Are Dissolved in Nonpolar Solvents, Anal. Chem. 63:1781–1788 (1991).CrossRefGoogle Scholar
  4. 4.
    Cheng, C., M.L. Gross, and E. Pittenauer, Complete Structural Elucidation of Triacylglycerols by Tandem Sector Mass Spectrometry, 70:4417–4426 (1998).CrossRefGoogle Scholar
  5. 5.
    Hsu, F.-F., and J. Turk, Structural Characterization of Triacylglycerols as Lithiated Adducts by Electrospray Ionization Mass Spectrometry Using Low-Energy Collisionally Activated Dissociation on a Triple Stage Quadrupole Instrument, J. Am. Soc. Mass Spectrom. 10:587–599 (1999).CrossRefGoogle Scholar
  6. 6.
    Marzilli, L.A., F. Dionisi, and P. Vouros, A Study of Triglycerides Using Ion Trap MS, Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics, American Society for Mass Spectrometry, Santa Fe, p. 1380.Google Scholar
  7. 7.
    Dorschel, C., Characterization of Triacylglycerols in Natural Fats and Oils by Neutral Loss and Product Ion ESI-MS-MS, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, American Society for Mass Spectrometry, Santa Fe (abstract number MPH 250).Google Scholar
  8. 8.
    Myher, J.J., Kuksis, A., Marai, L., and Manganaro, F., Quantitation of Natural Triacylglycerols by Reversed-Phase Liquid Chromatography with Direct Liquid Inlet Mass Spectrometry, J. Chromatogr. 283:289–301 (1984).CrossRefGoogle Scholar
  9. 9.
    Sempore, G., and J. Bezard, Qualitative and Quantitative Analysis of Peanut Oil Triacylglycerols by Reversed-Phase Liquid Chromatography, J. Chromatogr. 366:261–282 (1986).CrossRefGoogle Scholar
  10. 10.
    Singleton, J.A., and H.E. Patee, Characterization of Peanut Oil Triacylglycerols by HPLC, GLC, and EIMS, J. Am. Oil Chem. Soc. 64:534–538 (1987).Google Scholar
  11. 11.
    Rezanka, T., and P. Mares, Determination of Plant Triacylglycerols Using Capillary Gas Chromatography, High-Performance Liquid Chromatography and Mass Spectrometry, J. Chromatogr. 542:145–159 (1991).CrossRefGoogle Scholar
  12. 12.
    Barron, L.J.R., and G. Santa-Maria, Non-aqueous Reversed-Phase HPLC Analysis of Triglycerides, Chromatographia 23: 209–214 (1987).CrossRefGoogle Scholar
  13. 13.
    Mottram, H.R., S.E. Woodbury, and R.P. Evershed, Identification of Triacylglycerol Positional Isomers Present in Vegetable Oils by High Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry, Rapid Commun. Mass Spectrom. 11:1240–1252 (1997).CrossRefGoogle Scholar
  14. 14.
    Pittenauer, E., J. Bailer, K. Widhalm, and G. Allmaier, Analysis of Oleic Acid-Rich Edible Oils by Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, American Society for Mass Spectrometry, Santa Fe (abstract number TPH 303).Google Scholar

Copyright information

© AOCS Press 2002

Authors and Affiliations

  1. 1.Waters CorporationMilford

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