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Enzymatic process for extracting oil and protein from rice bran

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Journal of the American Oil Chemists' Society

Abstract

Enzymatic extraction of oil and protein from rice bran, using a commercial protease (Alcalase), was investigated and evaluated by response surface methodology. The effect of enzyme concentration was most significant on oil and protein extraction yields, whereas incubation time and temperature had no significant effect. The maximal extraction yields of oil and protein were 79 and 68%, respectively. Further, the quality of oil recovered from the process in terms of free fatty acid, iodine value, and saponification value was comparable with solvent-extracted oil and commercial rice bran oil, but the peroxide value was higher.

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References

  1. Christensen, F.M., Extraction by Aqueous Enzymatic Processes, inform 1:984–987 (1991).

    Google Scholar 

  2. Dominguez, H., M.J. Nunez, and J.M. Lema, Enzymatic Pretreatment to Enhance Oil Extraction from Fruits and Oilseeds: A Revie, Food Chem. 49:271–286 (1994).

    Article  CAS  Google Scholar 

  3. Rosenthal, A., D.L. Pyle, and K. Niranjan, Aqueous and Enzymatic Processes for Edible Oil Extraction, Enzyme Microb. Technol. 19:402–420 (1996).

    Article  CAS  Google Scholar 

  4. Buenrostro, M., and C.A. Lopez-Munguia, Enzymatic Extraction of Avocado Oil, Biotechnol. Lett. 8:505–506 (1986).

    Article  CAS  Google Scholar 

  5. McGlone, O.C., C.A. Lopez-Munguia, and J.V. Carter, Coconut Oil Extraction by a New Enzymatic Process, J. Food Sci. 51:695–697 (1986).

    Article  CAS  Google Scholar 

  6. Barrios, V.A., D.A. Olmos, R.A. Noyola, and C.A. Lopez-Munguia, Optimization of an Enzymatic Process for Coconut Oil Extraction, Oleagineux 45:35–42 (1990).

    CAS  Google Scholar 

  7. Che Man, Y.B. Suhardiyono, A.B. Asbi, M.N. Azudin, and L.S. Wei, Aqueous Enzymatic Extraction of Coconut Oil, J. Am. Oil Chem. Soc. 73:683–686 (1996).

    Article  Google Scholar 

  8. Tano-Debrah, K., and Y. Ohta, Aqueous Extraction of Coconut Oil by an Enzyme-Assisted Process, J. Sci. Food Agric. 74:497–502 (1997).

    Article  CAS  Google Scholar 

  9. Karlovic, D., M. Bocevska, J. Jakovlevic, and J. Turkulov, Corn Germ Oil Extraction by a New Enzymatic Process, Acta Aliment. 23:389–400 (1994).

    CAS  Google Scholar 

  10. Sarker, B.C., B.P.N. Singh, Y.C. Agrawal, and D.K. Gupta, Optimization of Enzyme Pretreatment of Rapeseed for Enhanced Oil Recovery, J. Food Sci. Technol. 35:183–186 (1998).

    CAS  Google Scholar 

  11. Dominguez, H., M.J. Nunez, and J.M. Lema, Oil Extractability from Enzymatically Treated Soybean and Sunflower: Range of Operational Variables, Food Chem. 46:277–284 (1993).

    Article  CAS  Google Scholar 

  12. Kashyap, M.C., Y.C. Agrawal, B.C. Sarkar, and B.P.N. Singh, Response Surface Analysis of Enzyme Aided Extraction of Soybean, J. Food Sci. Technol. 34:386–390 (1997).

    CAS  Google Scholar 

  13. Dominguez, H., M.J. Nunez, and J.M. Lema, Aqueous Processing of Sunflower Kernels with Enzymatic Technology, Food Chem. 53:427–434 (1995).

    Article  CAS  Google Scholar 

  14. Sineiro, J., H. Dominguez, M.J. Nunez, and J.M. Lema, Optimization of the Enzymatic Treatment During Aqueous Oil Extraction from Sunflower Seeds, —Ibid. 61:467–474 (1998).

    Article  CAS  Google Scholar 

  15. Singh, R.K., B.C. Sarker, B.K. Kumbhar, Y.C. Agrawal, and M.K. Kulshreshtha, Response Surface Analysis of Enzyme Assisted Oil Extraction Factors for Sesame, Groundnut and Sunflowers Seeds, J. Food Sci. Technol. 36:511–514 (1999).

    Google Scholar 

  16. Sengupta, R., and D.K. Bhattacharyya, Enzymatic Extraction of Mustard Seed and Rice Bran, J. Am. Oil Chem. Soc. 73:687–692 (1996).

    Article  CAS  Google Scholar 

  17. Hernandez, N., M.E. Rodriguez-Alegria, F. Gonzalez, and A. Lopez-Munguia, Enzymatic Treatment of Rice Bran to Improve Processing, —Ibid. 77:177–180 (2000).

    CAS  Google Scholar 

  18. Myers, R.H., and D.C. Montgomery, Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley & Sons, Inc., Chichester, 1995, pp. 279–350.

    Google Scholar 

  19. Hanmoungjai, P., L. Pyle, and K. Niranjan, Extraction of Rice Bran Oil Using Aqueous Media, J. Chem. Technol. Biotechnol. 75:348–52 (2000).

    Article  CAS  Google Scholar 

  20. Association of Official Analytical chemists, Official Methods of Analysis, 16th edn., edited by P. Cunniff, AOAC International, Gaithersburg, 1995.

    Google Scholar 

  21. International Union for Pure and Applied Chemistry, Standard Methods for the Analysis of Oils, Fats and Derivatives, 7th edn., edited by C. Paquot and A. Hautfenne, Blackwell Scientific, Oxford, 1986.

    Google Scholar 

  22. Seetharamaiah, G.S., and J.V. Prabhakar, Oryzanol Content of Indian Rice Bran Oil and Its Extraction from Soap Stock, J. Food Sci. Technol. 23:270–273 (1986).

    CAS  Google Scholar 

  23. Long, A.R., S.J. Massie, and W.J. Tyznik, Rapid Direct Extraction Derivatization Method for the Determination of Acylglycerol Lipids in Selected Sample Materials, J. Food Sci. 53:940–959 (1988).

    Article  CAS  Google Scholar 

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Correspondence to K. Niranjan.

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Hanmoungjai, P., Pyle, D.L. & Niranjan, K. Enzymatic process for extracting oil and protein from rice bran. J Amer Oil Chem Soc 78, 817–821 (2001). https://doi.org/10.1007/s11746-001-0348-2

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  • DOI: https://doi.org/10.1007/s11746-001-0348-2

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