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Antioxidant activities of major components of γ-oryzanol from rice bran using a linoleic acid model

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Abstract

The change in hydroperoxides of linoleic acid incubated with constant micro air flow at 37°C was used to evaluate the antioxidant activities of three major components of γ-oryzanol from rice bran (cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, and campesteryl ferulate) compared with α-tocopherol and ferulic acid. The four hydroperoxide isomers of linoleic acid, 9-hydroperoxy-10-trans, 12-cis-octadecadienoic acid [9HPODE(t,c)], 9-hydroperoxy-10-trans, 12-trans-octadecadienoic acid, 13-hydroperoxy-9-cis, 11-trans-octadecadienoic acid [13HPODE(c,t)], and 13-hydroperoxy-9-trans, 11-trans-octadecadienoic acid, were measured using normal-phase high-performance liquid chromatography with an ultraviolet detector. The three components of γ-oryzanol evidenced significant antioxidant activity when they were mixed with linoleic acid in a molar ratio of 1∶100 and 1∶250 but not in a molar ratio of 1∶500 (P<0.05). α-Tocopherol and ferulic acid also demonstrated significant antioxidant activity at all three molar ratios (P<0.05). The highest molar ratio (1∶100) of α-tocopherol, however, caused greater levels of 9HPODE(t,c) and 13HPODE(c,t) than the other two less concentrated treatments.

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Correspondence to J. Samuel Godber.

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Xu, Z., Godber, J.S. Antioxidant activities of major components of γ-oryzanol from rice bran using a linoleic acid model. J Amer Oil Chem Soc 78, 645 (2001). https://doi.org/10.1007/s11746-001-0320-1

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Key Words

  • Antioxidant
  • hydroperoxide
  • linoleic acid
  • γ-oryzanol