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Antioxidant activities of major components of γ-oryzanol from rice bran using a linoleic acid model

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The change in hydroperoxides of linoleic acid incubated with constant micro air flow at 37°C was used to evaluate the antioxidant activities of three major components of γ-oryzanol from rice bran (cycloartenyl ferulate, 24-methylene cycloartanyl ferulate, and campesteryl ferulate) compared with α-tocopherol and ferulic acid. The four hydroperoxide isomers of linoleic acid, 9-hydroperoxy-10-trans, 12-cis-octadecadienoic acid [9HPODE(t,c)], 9-hydroperoxy-10-trans, 12-trans-octadecadienoic acid, 13-hydroperoxy-9-cis, 11-trans-octadecadienoic acid [13HPODE(c,t)], and 13-hydroperoxy-9-trans, 11-trans-octadecadienoic acid, were measured using normal-phase high-performance liquid chromatography with an ultraviolet detector. The three components of γ-oryzanol evidenced significant antioxidant activity when they were mixed with linoleic acid in a molar ratio of 1∶100 and 1∶250 but not in a molar ratio of 1∶500 (P<0.05). α-Tocopherol and ferulic acid also demonstrated significant antioxidant activity at all three molar ratios (P<0.05). The highest molar ratio (1∶100) of α-tocopherol, however, caused greater levels of 9HPODE(t,c) and 13HPODE(c,t) than the other two less concentrated treatments.

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  1. 1.

    Gerhardt, A.L., and N.B. Gallo, Full-fat Rice Bran and Oat Bran Similarly Reduce Hypercholesterolemia in Humans, J. Nutr. 128:865–869 (1998).

    CAS  Google Scholar 

  2. 2.

    Seetharamaiah, G.S., and N. Chandrasekhara, Studies on Hypocholesterolemic Activity of Rice Bran Oil, Atherosclerosis 78:219–235 (1989).

    CAS  Article  Google Scholar 

  3. 3.

    Sugano, M., and E. Tsuji, Rice Bran Oil and Cholesterol Metabolism, J. Nutr. 127:521S-524S (1997).

    CAS  Google Scholar 

  4. 4.

    Xu, Z., and J.S. Godber, Purification and Identification of Components of γ-Oryzanol in Rice Bran Oil, J. Agric. Food Chem. 47:2724–2728 (1999).

    CAS  Article  Google Scholar 

  5. 5.

    Cuvelier, M.E., H. Richard, and C. Berset, Comparison of the Antioxidative Activity of Some Acid-Phenols: Structure-Activity Relationship, Biosci. Biotech. Biochem. 56:324–330 (1992).

    CAS  Article  Google Scholar 

  6. 6.

    Marinova, E.M., and N.V. Yanishlieva, Effect of Temperature on the Antioxidative Action of Inhibitors in Lipid Autoxidation, J. Sci. Food Agric. 60:313–318 (1992).

    CAS  Article  Google Scholar 

  7. 7.

    Marinova, E.M., and N.V. Yanishlieva, Effect of Lipid Unsaturation on the Antioxidative Activity of Some Phenolic Acids, J. Am. Oil Chem. Soc. 71:427–434 (1994).

    CAS  Google Scholar 

  8. 8.

    Pratt, D.E., Water-Soluble Antioxidant Activity in Soybeans, J. Food Sci. 37:322–323 (1972).

    Article  Google Scholar 

  9. 9.

    Ohta, T., S. Yamasaki, Y. Egashira, and H. Sanada, Antioxidative Activity of Corn Bran Hemicellulose Fragments, J. Agric. Food Chem. 42:653–656 (1994).

    CAS  Article  Google Scholar 

  10. 10.

    Frankel, E.N., Recent Advances in Lipid Oxidation, J. Sci. Food Agric. 54:495–500 (1991).

    CAS  Article  Google Scholar 

  11. 11.

    Porter, N.A., S.E. Caldwell, and K.A. Mills, Mechanisms of Free Radical Oxidation of Unsaturated Lipids, Lipids 30:277–290 (1995).

    CAS  Google Scholar 

  12. 12.

    Makinen, M., A. Kamal-Eldin, A.-M. Lampi, and A. Hopia, Effects of α- and γ-Tocopherols on Formation of Hydroperoxides and Two Decomposition Products from Methyl Linoleate, J. Am. Oil Chem. Soc. 77:801–806 (2000).

    CAS  Google Scholar 

  13. 13.

    Hopia, A., S.W. Huang, and E.N. Frankel, Effect of α-Tocopherol and Trolox on the Decomposition of Methyl Linoleate Hydroperoxides, Lipids 31:357–365 (1996).

    CAS  Article  Google Scholar 

  14. 14.

    Jung, M.Y., and D.B. Min, Effects of α-, γ-, and β-Tocopherols on Oxidative Stability of Soybean Oil, J. Food Sci. 55:1464–1465 (1990).

    CAS  Article  Google Scholar 

  15. 15.

    Shin, T., J.S. Godber, D.E. Martin, and J.H. Wells, Hydrolytic Stability and Changes in E Vitamers and Oryzanol of Extruded Rice Bran During Storage, Ibid. 62:704–708 (1997).

    CAS  Article  Google Scholar 

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Correspondence to J. Samuel Godber.

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Xu, Z., Godber, J.S. Antioxidant activities of major components of γ-oryzanol from rice bran using a linoleic acid model. J Amer Oil Chem Soc 78, 645 (2001).

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Key Words

  • Antioxidant
  • hydroperoxide
  • linoleic acid
  • γ-oryzanol