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Profile of polyunsaturated fatty acids produced by Thraustochytrium sp. KK17-3

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Journal of the American Oil Chemists' Society

Abstract

More than 300 strains of microorganisms producing polyunsaturated fatty acids (PUFA) were newly isolated from coastal seawater in the Seto Inland Sea and around Iriomote Island, Japan, by the baiting method. The profiles of PUFA from docosahexaenoic acid (DHA)-producing strains could be classified into four types. A strain, named KK17-3, was chosen for further study owing to its high DHA content (52.1% of total fatty acid) and wide range of PUFA (76.1%) including arachidonic, eicosapentaenoic, and docosapentaenoic acids as well as DHA. Glucose and tryptone were the optimal carbon and nitrogen sources, respectively, in a medium with salinity at 75% that of seawater. The PUFA contents in polar lipids (22.1% of total lipid), in which the DHA content was 39.3%, were higher than those in neutral lipids and glycolipids. Molecular phylogenetic analysis of 18S rRNA gene sequences showed KK17-3 to be a thraustochytrid. It also was observed to possess a life cycle composed of vegetative cells without successive bipartition, zoosporangium, and zoospore stage. Classification by the chemotaxonomic criterion based on PUFA compositions also supported this assignment.

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Authors and Affiliations

  1. Biological Resources Laboratory, National Institute of Advanced Industrial Science and Technology, 305-8566, Tsukuba, Ibaraki, Japan

    Toshihiro Yokochi

  2. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-4-1 Kagamiyama, 739-8527, Higashi-Hiroshima, Japan

    Jianzhong Huang, Tsunehiro Aki, Kazutaka Hachida, Seiji Kawamoto, Seiko Shigeta, Kazuhisa Ono & Osamu Suzuki

Authors
  1. Jianzhong Huang
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  2. Tsunehiro Aki
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  3. Kazutaka Hachida
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  4. Toshihiro Yokochi
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  5. Seiji Kawamoto
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  7. Kazuhisa Ono
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  8. Osamu Suzuki
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Correspondence to Tsunehiro Aki.

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Huang, J., Aki, T., Hachida, K. et al. Profile of polyunsaturated fatty acids produced by Thraustochytrium sp. KK17-3. J Amer Oil Chem Soc 78, 605–610 (2001). https://doi.org/10.1007/s11746-001-0312-1

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  • DOI: https://doi.org/10.1007/s11746-001-0312-1

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