Abstract
The effects of temperature and temperature shift on the fatty acid composition and docosahexaenoic acid (DHA, C22∶6n−3) content and productivity of the marine microalga Crypthecodinium cohnii ATCC 30556 were investigated. The microalga grew well over the entire range of temperatures (15–30°C) studied. High temperature favored the growth of the microalga with the highest specific growth rate of 0.092 h−1 at 30°C. In contrast, low temperature favored the formation of polyunsaturated fatty acids. The highest DNA content was obtained at 15°C in the early stationary phase (i.e., 72h). In order to achieve high DHA productivity, a shift from high temperature to low temperature at a later stage of cultivation (i.e., 48h) was also attempted. A temperature shift from 25°C (for 48 h) to 15°C (for 24 h) resulted in an increase in cellular DHA content by 19.9% and productivity by 6.5% as compared to that maintained at 25°C (for 72 h).
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Jiang, Y., Chen, F. Effects of temperature and temperature shift on docosahexaenoic acid production by the marine microalge Crypthecodinium cohnii . J Amer Oil Chem Soc 77, 613–617 (2000). https://doi.org/10.1007/s11746-000-0099-0
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DOI: https://doi.org/10.1007/s11746-000-0099-0