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Determination of trans fatty acids and fatty acid profiles in margarines marketed in spain

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Journal of the American Oil Chemists' Society

Abstract

Two gas chromatography (GC) procedures were compared for routine analysis of trans fatty acids (TFA) of vegetable margarines, one direct with a 100-m high-polarity column and the other using argentation thin-layer chromatography and GC. There was no difference (P>0.05) in the total trans 18∶1 percentage of margarines with a medium level of TFA (∼18%) made using either of the procedures. Both methods offer good repeatability for determination of total trans 18∶1 percentage. The recoveries of total trans isomers of 18∶1 were not influenced (P>0.1) by the method used. Fatty acid composition of 12 Spanish margarines was determined by the direct GC method. The total contents of trans isomers of oleic, linoleic, and linolenic acids ranged from 0.15 to 20.21, from 0.24 to 0.99, and from 0 to 0.47%, respectively, and the mean values were 8.18, 0.49, and 0.21%. The mean values for the ratios [cis-polyunsaturated/(saturated +TFA)] and [(cis-polyunsaturated + cis-monounsaturated)/(saturated +TFA)] were 1.25±0.39 and 1.92±0.43, respectively. Taking into account the annual per capita consumption of vegetable margarine, the mean fat content of the margarines (63.5%), and the mean total TFA content (8.87%), the daily per capita consumption of TFA from vegetable margarines by Spaniards was estimated at about 0.2 g/person/d.

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References

  1. Haummann, B.F., Tools: Hydrogenation, Interesterification, INFORM 5:668–678 (1994).

    Google Scholar 

  2. Petrauskaite, V., W. de Grey, M. Kellens, and A. Huyghebaert, Physical and Chemical Properties of trans-Free Fats Produced by Chemical Interesterification of Vegetable Oil Blends, J. Am. Oil Chem. Soc. 75:489–493 (1998).

    CAS  Google Scholar 

  3. Kellens, M., Developments in Fat Modification Technology: Interesterification of Oils and Fats, presented at Workshop on Oils Interesterification, Texas A&M University, pp. 1–2 (1996).

  4. Willett, W.C., M.J. Stampfer, J.E. Manson, G.A. Golditz, F.E. Speizer, B.A. Rosner, L.A. Sampson, and C.H. Hennikins, Intake of trans Fatty Acids and Risk of Coronary Heart Disease Among Women, Lancet 341:581–585 (1993).

    Article  CAS  Google Scholar 

  5. Siguel, E.N., and R.H. Lerman, Trans Fatty Acid Patterns in Patients with Angiographically Documented Coronary Artery Disease, Am. J. Cardiol. 71:916–920 (1993).

    Article  CAS  Google Scholar 

  6. Judd, J.T., B.A. Clevidence, R.A. Muesing, J. Wittes, M.E. Sunkin, and J.J. Podczasy, Dietary trans Fatty Acids: Effects of Plasma Lipids and Lipoproteins of Healthy Men and Women, Am. J. Clin. Nutr. 59:861–868 (1994).

    CAS  Google Scholar 

  7. Wolff, R.L., Content and Distribution of trans-18∶1 Acids in Ruminant Milk and Meat Fats. Their Importance in European Diets and Their Effect on Human Milk, J. Am. Oil Chem. Soc. 72:259–272 (1995).

    CAS  Google Scholar 

  8. Hunter, J.E., and T.H. Applewhite, Reassessment of trans Fatty Acid Availability in the US Diet, Am. J. Clin. Nutr. 54:363–369 (1991).

    CAS  Google Scholar 

  9. Ratnayake, W.M.N., G. Pelletier, R. Hollywood, S. Bacler, and D. Leyte, Trans Fatty Acids in Canadian Margarines: Recent Trends, J. Am. Oil Chem. Soc. 75:1587–1594 (1998).

    CAS  Google Scholar 

  10. Leth, T., L. Ovesen, and K. Hansen, Fatty Acid Composition of Meat from Ruminants with Special Emphasis on trans Fatty Acids, 75:489–493 (1998).

    Google Scholar 

  11. Craig-Schmidt, M.C., Consumption of trans Fatty Acids, in Trans Fatty Acids in Human Nutrition, edited by J.L. Sebedio and W.W. Christie, The Oily Press, Dundee Scotland, 1998, pp. 59–113.

    Google Scholar 

  12. Firestone, D., and A. Sheppard, Determination of trans Fatty Acids, in Advances in Lipid Methodology, edited by W. W. Christie, The Oily Press, Ayr, 1992, Vol. 1, pp. 273–322.

    Google Scholar 

  13. Ulberth, F., and H.J. Haider, Determination of Low Level trans Unsaturation in Fats by Fourier Transform Infrared Spectroscopy, J. Food Sci. 57:1444–1447 (1992).

    Article  CAS  Google Scholar 

  14. Ulberth, F., and M. Henninger, Quantitation of trans Fatty Acids in Milk Fat Using Spectroscopic and Chromatographic Methods, J. Dairy Res. 61:517–527 (1994).

    Article  CAS  Google Scholar 

  15. Molkentin, J., and D. Precht, Optimized Analysis of trans-Octadecenoic Acids in Edible Fats, Chromatographia 41:267–272 (1995).

    CAS  Google Scholar 

  16. Precht, D., and J. Molkentin, Rapid Analysis of the Isomers of trans-Octadecenoic Acid in Milk Fat, Int. Dairy J. 6:791–809 (1996).

    Article  CAS  Google Scholar 

  17. Christie, W.W., and G.H.M. Breckenridge, Separation of cis and trans Isomers of Unsaturated Fatty Acids by High-Performance Liquid Chromatography in the Silver Ion Mode, J. Chromatogr. 469:261–269 (1989).

    Article  CAS  Google Scholar 

  18. Adlof, R.O., L.C. Copes, and E.A. Emken, Analysis of the Monoenic Fatty Acid Distribution in Hydrogenated Vegetable Oils by Silver-Ion High-Performance Liquid Chromatography, J. Am. Oil Chem. Soc. 72:571–574 (1995).

    CAS  Google Scholar 

  19. Ulberth, F., and M. Henninger, Simplified Method for the Determination of trans Monoenes in Edible Fats by Thin-Layer Chromatography-Gas-Liquid Chromatography, 69:829–831 (1992).

    CAS  Google Scholar 

  20. Ratnayake, W.M.N., Analysis of trans Fatty acids, in Trans Fatty Acids in Human Nutrition, edited by J.L. Sebedio and W.W. Christie, The Oily Press, Dundee, 1998, pp. 115–162.

    Google Scholar 

  21. Christopherson, S.W., and R.L. Glass, Preparation of Milk Fat Methyl Esters by Alcoholysis in an Essentially Non-Alcoholic Solution, J. Dairy Sci. 52:1289–1290 (1969).

    Article  CAS  Google Scholar 

  22. Bayard, C.C., and R.L. Wolff, Trans-18∶1 Acids in French Tub Margarines and Shortening: Recent Trends, J. Am. Oil Chem. Soc. 72:1485–1489 (1995).

    CAS  Google Scholar 

  23. Henninger, M., and F. Ulberth, Trans Fatty Acids in Margarines and Shortenings Marketed in Austria, Z. Lebensm. Unters. Forsch. 203:210–215 (1996).

    Article  CAS  Google Scholar 

  24. Molkentin, J., and D. Precht, Occurrence of trans-C16∶1 Acids in Bovine Milkfats and Partially Hydrogenated Edible Fats, Milchwissenschaft 57:380–384 (1997).

    Google Scholar 

  25. Ali, L.A., G. Angyal, C.M. Weaver, J.I. Rader, and M.M. Mossoba, Determination of Total trans Fatty Acids in Foods: Comparison of Capillary-Column Gas Chromatography and Single-Bounce Horizontal Attenuated Total Reflection Infrared Spectroscopy, J. Am. Oil Chem. Soc. 73:1699–1705 (1996).

    Article  CAS  Google Scholar 

  26. Ovensen, L., T. Leth, and K. Hansen, Fatty Acid Composition of Danish Margarines and Shortenings, with Special Emphasis on trans Fatty Acids, Lipids 31:971–975 (1998).

    Article  Google Scholar 

  27. Gurr, M.I., Trans Fatty Acids: Metabolic and Nutritional Significance, Int. Dairy Fed. Doc. 166:5–17 (1983).

    Google Scholar 

  28. Ackman, R.G., S.N. Hooper, and D.L. Hooper, Linolenic Acid Artifacts from Deodorization of Oils, J. Am. Oil Chem. Soc. 51:42–49 (1974).

    CAS  Google Scholar 

  29. Tsanev, R., A. Russeva, T. Rizov, and I. Dontcheva, Content of trans-Fatty Acids in Edible Margarines, 75:143–145 (1998).

    CAS  Google Scholar 

  30. Coll, L., and M.L. Gutiérrez, Determinación de Acidos Grasos Trans-insaturados en Margarinas y Mantequillas, An. Bromatol. 41:115–128 (1989).

    Google Scholar 

  31. Boatella, J., M. Rafecas, and R. Codony, Isomeric trans Fatty Acids in the Spanish Diet and Their Relationships with Changes in Fat Intake Patterns, Eur. J. Clin. Nutr. 47 (Suppl. 1):S62-S65 (1993).

    Google Scholar 

  32. Ministerio de Agricultura, Pesca y Alimentación Consumo Alimentario, edited by Secretaria General Técnica, Madrid, Spain 1997, pp. 233–235.

  33. Wolff, R.L., Contribution of trans-18∶1 Acids from Dairy Fat to European Diets, J. Am. Oil Chem. Soc. 71:277–283 (1994).

    CAS  Google Scholar 

  34. Poppel, van G., Intake of trans Fatty Acids in Western Europe: The TRANSFAIR Study, Lancet 351:1099 (1998).

    Google Scholar 

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Correspondence to L. Alonso.

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Alonso, L., Fraga, M.J. & Juárez, M. Determination of trans fatty acids and fatty acid profiles in margarines marketed in spain. J Amer Oil Chem Soc 77, 131–136 (2000). https://doi.org/10.1007/s11746-000-0022-8

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  • DOI: https://doi.org/10.1007/s11746-000-0022-8

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